Self-HLA-D-Region Products Restrict Human T-Lymphocyte Activation by Antigen

Thorsby, E.; Bergholtz, B. O.; Nousiainen, Helena

doi:10.1007/978-1-4684-3761-4_7

Cited by 4 publications

(1 citation statement)

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“…Among these, class I transplantation antigens (in humans, the HLA-ABC system) are expressed on almost all nucleated cells (1). In contrast, class II transplantation antigens (in humans, HLA-DR, HLA-DC, and HLA-SB, collectively often referred to as Ia antigens) are generally limited to cells involved in the immune system (2,3). These glycoproteins, when present in the plasma membrane of macrophages, participate in the presentation of foreign antigens to T cells (2,4).…”

Section: Introductionmentioning

confidence: 99%

Expression of class II transplantation antigen on vascular smooth muscle cells in human atherosclerosis.

Jonasson¹,

Holm²,

Skalli³

et al. 1985

J. Clin. Invest.

275

126

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A large proportion of the cells of the human atherosclerotic plaque is assumed to be derived from medial smooth muscle cells. In contrast to these, the cells of the plaque have the capacity to accumulate lipid, and they also proliferate at a higher rate than medial cells. It has therefore been suggested that smooth muscle cells undergo a change of phenotype during atherogenesis, but there has been no evidence for such a change on the molecular level. We have now analyzed carotid artery plaques using a battery of antibodies against cell surface and cytoskeletal antigens, and found that most of the cells express the class II transplantation antigen (Ia antigen) HLA-DR. Also, the beta chain of HLA-DR was detected by immunoblotting of plaque extracts with the OKIal nmonoclonal antibody. HLA-DR is normally present on cells of the immune system, but only 60% of the DR-positive cells of the plaque reacted with monoclonal antibodies specific for macrophages and lymphocytes. Many of the remaining DR-positive cells contained the muscle-specific intermediate filament protein, desmin. This indicates that smooth muscle cells of atherosclerotic plaques express DR antigen. In contrast, very few DRpositive cells were found in normal human arteries. This suggests that expression of class II antigen is part of a phenotypic change in smooth muscle cells in atherosclerosis.

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Section: Introductionmentioning

confidence: 99%

Expression of class II transplantation antigen on vascular smooth muscle cells in human atherosclerosis.

Jonasson¹,

Holm²,

Skalli³

et al. 1985

J. Clin. Invest.

275

126

View full text Add to dashboard Cite

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Domain structures and molecular evolution of class I and class II major histocompatibility gene complex (MHC) products deduced from amino acid and nucleotide sequence homologies

Ohnishi

1984

Origins Life Evol Biosphere

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Domain structures of class I and class II MHC products were analyzed from a viewpoint of amino acid and nucleotide sequence homologies. Alignment statistics revealed that class I (transplantation) antigen H chains consist of four mutually homologous domains, and that class II (HLA-DR) antigen beta and alpha chains are both composed of three mutually homologous ones. The N-terminal three and two domains of class I and class II (both beta and alpha) gene products, respectively, all of which being approximately 90 residues long, were concluded to be homologous to beta2-microglobulin (beta2M). The membrane-embedded C-terminal shorter domains of these MHC products were also found to be homologous to one another and to the third domain of class I H chains. Class I H chains were found to be more closely related to class II alpha chains than to class II beta chains. Based on these findings, an exon duplication history from a common ancestral gene encoding a beta2M-like primodial protein of one-domain-length up to the contemporary MHC products was proposed.

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