Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds

Balakrishnan, Biji; Jayakrishnan, A.

doi:10.1016/j.biomaterials.2004.10.005

Cited by 619 publications

(437 citation statements)

References 30 publications

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“…Briefly, in our eGels, there are two kinds of crosslinking: one is chemical crosslinking, which is realized by Schiff's base formation between the ε-amino groups of lysine or hydroxylysine side groups in gelatin and the aldehyde groups in OA/nEOAs; the other is the physical crosslinking, which is realized by the borate complexation with diols in OA/nEOAs and by the hydrogen bonding interactions between carboxyls, amine groups, hydroxyls, and amides in EOA/OA/gelatin. The details of aforementioned crosslinking mechanism were reported in the publications of Balakrishnan et al 9,23,29 In this study, (nEOAs + OA) at a concentration of 0.20 g/mL in 0.1 M borax and gelatin at a concentration of 0.15 g/mL in water were mixed to get hydrogels, and the feeding component was presented in Table 1. Such hydrogel system was chosen because Balakrishnan et al demonstrated that the hydrogel made from OA in 0.1 M borax + gelatin in water had good in vitro and in vivo biocompatibility and was suitable for cartilage regeneration, drug release, and wound dressing as an injectable adhesive biomimetic scaffold.…”

Section: Synthesis Of Egelmentioning

confidence: 97%

“…The scheme for hydrogel preparation was presented in Figure 1A. The gel time, which was defined as the time taken for the formed gel to stop the stir bar according to Balakrishnan and Jayakrishnan 23 and Mo et al, 24 was recorded using a stopwatch. The gel properties were adjusted by the weight content of nEOAs in eGels.…”

Section: Preparation Of Electroactive Neoasmentioning

confidence: 99%

“…Such hydrogel system was chosen because Balakrishnan et al demonstrated that the hydrogel made from OA in 0.1 M borax + gelatin in water had good in vitro and in vivo biocompatibility and was suitable for cartilage regeneration, drug release, and wound dressing as an injectable adhesive biomimetic scaffold. 9,23,[29][30] To further improve its properties for new and wider clinical applications, we integrated electroactive degradable conducting polymer nanoparticle of nEOAs within this hydrogel. As presented in Table 1, adding nEOAs can easily adjust the gel time of hydrogels: the higher the weight content of nEOA in eGels, the longer the gel time.…”

Section: Synthesis Of Egelmentioning

confidence: 99%

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Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1 H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10 −6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release.

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Section: Synthesis Of Egelmentioning

confidence: 97%

Section: Preparation Of Electroactive Neoasmentioning

confidence: 99%

Section: Synthesis Of Egelmentioning

confidence: 99%

See 1 more Smart Citation

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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“…18,19 ADA is a partially oxidized product of alginate which facilitates the covalent crosslinking with gelatin through Schiff's base formation due to the reaction of free amino groups of lysine or hydroxylysine amino acid residues of gelatin and available aldehyde groups of ADA.…”

Section: 3mentioning

confidence: 99%

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

et al. 2014

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Microencapsulation of cells by using biodegradable hydrogels offers numerous attractive features for a variety of biomedical applications including tissue engineering. This study highlights the fabrication of microcapsules from an alginate-gelatin crosslinked hydrogel (ADA-GEL) and presents the evaluation of the physico-chemical properties of the new microcapsules which are relevant for designing suitable microcapsules for tissue engineering. Alginate di-aldehyde (ADA) was synthesized by periodate oxidation of alginate which facilitates crosslinking with gelatin through Schiff's base formation between the free amino groups of gelatin and the available aldehyde groups of ADA. Formation of Schiff's base in ADA-GEL and aldehyde groups in ADA was confirmed by FTIR and NMR spectroscopy, respectively. Thermal degradation behavior of films and microcapsules fabricated from alginate, ADA and ADA-GEL was dependent on the hydrogel composition. The gelation time of ADA-GEL was found to decrease with increasing gelatin content. The swelling ratio of ADA-GEL microcapsules of all compositions was significantly decreased, whereas the degradability was found to increase with the increase of gelatin ratio. The surface morphology of the ADA-GEL microcapsules was totally different from that of alginate and ADA microcapsules, observed by SEM. Two different buffer solutions (with and without calcium salt) have an influence on the stability of microcapsules which had a significant effect on the gelatin release profile of ADA-GEL microcapsules in these two buffer solutions.

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“…Seeded cell proliferate and differentiate to form tissues. Such approach in tissue engineering reduces the surgical operations (Balakrishnan and Jayakrishnan, 2005;Kretlow et al, 2007). Bombyx mori silk, a member of Bombycidae family is composed of a filament core fibroin proteins cemented together with glue-like sericine proteins (Kaplan et al, 1994;Magoshi et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization and in vitro study of biocompatible fibroin hydrogel

Sah¹,

Pramanik²

2011

Afr. J. Biotechnol.

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In this study, Bombyx mori silk based hydrogels were prepared and their biorelevant properties like physical, chemical and thermal properties were studied. Firstly, silk fibroin aqueous solution was prepared and the molecular weight of fibroin protein was determined followed by particle size analysis for the confirmation of study. Silk fibroin hydrogels were prepared by treating a 12% (w/v) silk fibroin aqueous solution at 4°C (thermgel) and lyophilized. The swelling and thermorheological behaviour of fibroin hydrogels were studied. The morphology and crystalline structure of lyophilized hydrogels were investigated by scanning electron microscopy (SEM) and wide-angle diffractometry, respectively while the surface functional groups were analyzed by FT-IR. The thermal behavior was also studied by means of differential scanning calorimetry and gravimetric method. The cytocompatibility of the hydrogels was evaluated through three-dimensional culture with human peripheral blood mononuclear cells. Lyophilized fibroin gel of high strength and high thermal stability were obtained. The β-crystelline structure of lyophilized fibroin hydrogel has shown excellent swelling capacity to mimic the living tissues. The surfaces of these hydrogels were found supporting to cell adherence and proliferation. hMNCs could survive and proliferate in the gel within 3 weeks, and the gel had good cytocompatibility. It was concluded that fibroin hydrogel not only has interpenetrating network structure but also has good cytocompatibility and could be used as injectable scaffolds able to promote in situ bone regeneration.

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Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds

Cited by 619 publications

References 30 publications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

Preparation, characterization and in vitro study of biocompatible fibroin hydrogel

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