Self-compatibility in aLycopersicon peruvianum variant (LA2157) is associated with a lack of style S-RNase activity

Kowyama, Yasuo; Kunz, Caroline; Lewis, I. M.; Newbigin, Ed; Clarke, Adrienne E.; Anderson, Marilyn A.

doi:10.1007/bf01253997

Cited by 73 publications

(55 citation statements)

References 27 publications

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“…We used the S. arcanum accessions LA2163 (SI) and LA2157 (SC), which are similar in most respects, apart from mating system (Rick 1986). These two accessions are cross-compatible in either direction (Rick 1986;Kowyama et al 1994). Importantly, LA2157 expresses a mutant S-RNase that lacks RNase activity Royo et al 1994) and confers self-compatibility (Rivers and Bernatzky 1994).…”

Section: Resultsmentioning

confidence: 99%

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

Chetelat

2014

Genetics

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We previously isolated a pollen factor, ui6.1, which encodes a Cullin1 protein (CUL1) that functions in unilateral interspecific incompatibility (UI) in Solanum. Here we show that CUL1 is also required for pollen function in self-incompatibility (SI). We used RNA interference (RNAi) to reduce CUL1 expression in pollen of Solanum arcanum, a wild SI tomato relative. Hemizygous T 0 plants showed little or no transmission of the transfer DNA (T-DNA) through pollen when crossed onto nontransgenic SI plants, indicating that CUL1-deficient pollen are selectively eliminated. When crossed onto a related self-compatible (SC) accession lacking active S-RNase, pollen transmission of the T-DNA followed Mendelian ratios. These results provide further evidence for functional overlap between SI and UI on the pollen side and suggest that CUL1 mutations will reinforce SI-to-SC transitions in natural populations only if preceded by loss of pistil S-RNase expression.S ELF-INCOMPATIBILITY (SI) is a widespread genetic mechanism in hermaphroditic plants that allows for the recognition and rejection of closely related pollen to prevent inbreeding. The breakdown of SI to self-compatibility (SC) through mutation occurs frequently (Igic et al. 2008), presumably driven by reproductive assurance under conditions where pollen from compatible mates is limiting. Pollen from SC species or populations is typically rejected on pistils of related SI species or populations, while, in the reciprocal crosses (SC pollinated by SI), no pollen rejection occurs. This pattern of unilateral incompatibility (UI) is known as the "SI 3 SC rule" (Lewis and Crowe 1958). While the mechanisms underlying SI have been the subject of much investigation, pollen rejection by UI is less well understood.The cultivated tomato (Solanum lycopersicum) and related wild Solanum species provide a powerful system with which to study these reproductive barriers. They exhibit a wide range of mating systems, including SI-enforced obligate outcrossing, SC with facultative outcrossing, and SC with high levels of inbreeding (Rick 1988). Self-compatible biotypes or accessions of mostly SI species provide a source of natural variation for studying SI-related factors.Self-incompatibility in Solanum and other Solanaceae is the S-RNase based, gametophytic type, in which S-specificity is determined by S-RNases in the pistil (McClure et al. 1989) and S-locus F-box proteins (SLFs) in pollen (Sijacic et al. 2004). F-box proteins, together with Skp1 and Cullin1 proteins, are components of Skp, Cullin, Fbox type (SCF) ubiquitin E3 ligases that mark proteins for degradation by the 26S proteasome (Zheng et al. 2002;Moon et al. 2004). The ubiquitinproteasome pathway is thought to regulate pollen-side SI responses in the Solanaceae (Zhang et al. 2009). In the "collaborative non-self-recognition" model (Kubo et al. 2010), the S-locus encodes multiple SLF proteins that together recognize different suites of S-RNases. In compatible pollinations, SLF/SRNase interactions lead to protection of p...

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Section: Resultsmentioning

confidence: 99%

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

Chetelat

2014

Genetics

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“…Conserved regions C2 and C3 each contain a His residue important for RNase activity (Kawata et al, 1988;McClure et al, 1989). The observation that pollen RNA is specifically degraded after self-pollination led to the suggestion that S-RNases function as S-specific cytotoxins in SI (McClure et al, 1989(McClure et al, , 1990Gray et al, 1991;Huang et al, 1994;Kowyama et al, 1994). Although there is considerable evidence that SI in Solanaceae is based on S-RNase cytotoxicity, recent evidence suggests that other effects also may be important.…”

Section: S-rnase-based Simentioning

confidence: 99%

Pollen-Pistil Interactions and Their Role in Mate Selection

et al. 2016

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“…Transgenic experiments and mutant analyses in the family Solanaceae demonstrated that replacing the conserved His residues in S-RNase resulted in SC, indicating RNase activity is essential for S-RNase cytotoxicity during the SI reaction Kowyama et al, 1994). RNase activity in a Maleae SC haplotype is absent, possibly because of an amino acid insertion in the region facing the active site (Nyska et al, 2013).…”

Section: Identification and Characterization Of S-rnasementioning

confidence: 99%

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Matsumoto

Tao

2016

The Hortic J

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Prunus fruit tree species exhibit S-ribonuclease (S-RNase)-based gametophytic self-incompatibility (GSI). This system is also present in the families Plantaginaceae and Solanaceae and the tribe Maleae of the family Rosaceae. In S-RNase-based GSI, self/nonself-recognition between the pistil and pollen is controlled by the pistil S determinant S-ribonuclease gene (S-RNase) and the pollen S determinant F-box gene(s). Accumulated evidence indicates the Prunus pollen S locus contains a single F-box gene, while that of other plants consists of multiple F-box genes. The pollen S F-box genes are called S haplotype-specific F-box (SFB), S-locus F-box brothers (SFBB), and S-locus F-box (SLF) in Prunus, Maleae, and Solanaceae species, respectively. The consequences of pollen S gene mutations and heterodiallelic pollen production differ between Prunus species and other plants, suggesting there are different pollen S functions during self/nonself-recognition. The GSI systems of Prunus and other plants are believed to include the ubiquitin proteasome system for protein degradation. However, Prunus SFB is assumed to facilitate the S-RNase cytotoxic effects during selfrecognition, while SLFs and SFBBs are thought to function collaboratively during nonself-recognition to avoid S-RNase cytotoxicity. This review summarizes the distinct features of the S-RNase-based GSI mechanism in Prunus species, with special references to the recent advances in our understanding of S-RNase-based GSI.

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Self-compatibility in aLycopersicon peruvianum variant (LA2157) is associated with a lack of style S-RNase activity

Cited by 73 publications

References 27 publications

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

The Role of a Pollen-Expressed Cullin1 Protein in Gametophytic Self-Incompatibility in Solanum

Pollen-Pistil Interactions and Their Role in Mate Selection

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

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