Self-Assembly of Soluble Chitosan Derivatives Nanoparticles for Vaccine: Synthesis, Characterization and Evaluation

Abstract

Herein, a novel chitosan derivative nanoparticle was proposed to function as a delivery carrier. First of all, an improvement was made to the way N-2-hydroxypropyl trimcthyl ammonium chloride chitosan (N-2-HACC) was synthesized. Moreover, the solution to one-step synthesis of N-2-HACC from chitosan (CS) was developed. Different from the previous report, the synthesis process was simplified, and there was a reduction in the amount of 2,3-epoxypropyl trimethyl ammonium chloride (EPTAC) used. With its excellent water solubility maintained, the relatively low degree of substitution was controlled to facilitate the cross-linking reaction. The results obtained from 1H-NMR, FTIR spectroscopy, and XRD indicated a smooth EPTAC onto CS for the formation of N-2-HACC with 59.33% the degree of substitution (DS). According to our results, N-2-HACC could be dissolved in various organic solvents, deionized water, 1% acetic acid aqueous solution, and others at room temperature. Finally, a novel chitosan nanoparticle material was prepared using the self-assembly method with β-glycerophosphate sodium (β-GC), with excellent immune properties achieved, thus providing a new strategy for chitosan self-assembled nanoparticles.

Magnesium-Doped Nano-Hydroxyapatite/Polyvinyl Alcohol/Chitosan Composite Hydrogel: Preparation and Characterization

“…Experimental studies have shown that the presence of nanoparticles can alter the molecular structure of composite materials by cross-linking with CS on the polymer chain. 48 Additionally, it has been observed that water molecule diffusion within the polymer network also has a significant impact.…”

The numerical simulation investigates the influence of agitator parameters on particle suspension characteristics

“…Only in specific acidic liquids does the alkaline polysaccharide chitosan exhibit reasonably good solubility. Quaternization was used to alter chitosan to create quaternized chitosan, which increased its water solubility and expanded its uses [4][5][6][7]. Quaternary ammonium salt synthesis is a two-phase, solidliquid reaction that produces chitosan.…”