Self-assembly of pentapeptides in ethanol to develop organogels

Tan, Tingyuan; Shen, Zhiwei; Wang, Yujiao; Guo, Zhen; Hu, Jun; Zhang, Yi

doi:10.1039/d0sm01303j

Cited by 10 publications

(9 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low concentration hydrogel polymerized from acrylamide and water protects proteins from harsh detergent treatment (Chung et al, 2013;Treweek et al, 2015;Yang et al, 2014). Organogel is a gel composed of an organic solvent phase dispersed within a cross-linked polymer and was used for drug delivery or cosmetic products (Skilling et al, 2014;Tan et al, 2020;Yuan et al, 2019). TESOS method is its first application in the microscopic imaging field.…”

Section: Discussionmentioning

confidence: 99%

Mapping of individual sensory nerve axons from digits to spinal cord with the Transparent Embedding Solvent System

Men

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

SummaryUnderstanding the connections and projections of neurons has been a fundamental issue for neuroscience. Although strategies have been developed to map the projection of individual axons within the mouse brain, high resolution mapping of individual peripheral nerve axons in peripheral organs or spinal cord has never been achieved. Here, we designed the Transparent Embedding Solvent System (TESOS) method and developed a technical pipeline for imaging, reconstructing and analyzing large samples containing various tissue types at sub-micron resolution. The mouse whole body was reconstructed at micron-scale resolution. We were able to image, reconstruct and analyze the complete axonal projection of individual sensory neurons within an intact mouse paw or spinal cord at sub-micron resolution. Furtherly, we imaged and reconstructed the entire projection course of individual sensory neurons from adult mouse digits to the spinal cord. The TESOS method provides an efficient tool for micron-scale connectome mapping of the peripheral nervous system.

show abstract

Section: Discussionmentioning

confidence: 99%

Mapping of individual sensory nerve axons from digits to spinal cord with the Transparent Embedding Solvent System

Men

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Gelation of EAF-5 in Different Alcohols. EAF-5 was obtained as previously reported, 47 and its chemical structure is illustrated in Figure 1A. A wide variety of alcohols were chosen to evaluate the gelation behavior of EAF-5.…”

Section: ■ Reaults and Discussionmentioning

confidence: 99%

“…In a recent study, we reported a pentapeptide with a sequence of ECAYF (named EAF-5), which acted as an organogelator that was capable of self-assembling in ethanol to form a gel. 47 In this paper, we systematically studied the selfassembly of EAF-5 in a series of alcohol solvents. It was found that EAF-5 self-assembled into organogels in a series of alcohols except methanol.…”

Section: ■ Introductionmentioning

confidence: 99%

“…EAF-5 (Figure 1A) was designed based on the EF-5 (ECGYF) peptides that we reported previously 48 and synthesized with a purity of 98% (w/w) as verified by high-performance liquid chromatography (HPLC) and mass spectrometry. 47 All organic solvents (99.8%) were purchased from Sigma-Aldrich Corporation (USA) and were used without additional purification. All other chemicals were purchased from Sigma-Aldrich, unless specified otherwise.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gelation of a Pentapeptide in Alcohols

et al. 2021

Self Cite

View full text Add to dashboard Cite

Properties of solvents such as polarity and H-bond-forming ability are critical for the formation of an organogel and have a significant impact on the gel behavior, as solvents are the majority of organogel systems. However, so far, there is still a lack of systematic studies regarding the effects of molecular structures of solvents on the characteristics of organogels. Motivated by revealing such a relationship, in this paper, we studied the morphologies of assemblies, gelation behaviors, and secondary structures of a pentapeptide termed EAF-5 in a wide variety of alcohols. The side chains and lengths of carbon chains of the solvent molecules were found to play a critical role in the selfassembly and gelation of EAF-5. EAF-5 was capable of self-assembling into fibers and entangling into a network in alcohols including ethanol, propanol, butanol, n-pentanol, and n-hexanol, which further immobilized the corresponding alcohols to form gels. In these organogels, increasing β-sheet secondary structures of the peptides were formed by introducing side chains and extending the length of primary alcohol molecules. We hypothesized that alcohol molecules with extended lengths and side chains reduced the gelator−solvent interactions and promoted the gelator−gelator interactions, resulting in the self-assembly of EAF-5 into fibril structures and development of gels. These findings provide a new sight into the interactions between gelators and solvents and are helpful for designing peptide-based organogelators.

show abstract

“…One class of molecules with a known propensity for gelation are amides and amide-containing small molecules, oligomers, and polymers, including peptides and proteins . The fact that amides form organogels with a broad variety of solvents has sometimes been attributed to the highly polar nature of the amide bond that facilitates strong intermolecular interactions with polar organic solvents .…”

Section: Introductionmentioning

confidence: 99%

Sonication-Induced, Solvent-Selective Gelation of a 1,8-Napthalimide-Conjugated Amide: Structural Insights and Pollutant Removal Applications

et al. 2021

View full text Add to dashboard Cite

Reported herein is the synthesis, characterization, and dye removal applications of a highly solvent-selective organogel-forming amide, compound 1, which contains a 1,8-naphthalmide moiety, flexible n-hexyl chain, and benzene ring. This compound displayed remarkable solvent selectivity, with gel formation occurring only in the presence of alkylated aromatic solvents. Detailed structural characterization of the gels, combined with notable theoretical insights, is invoked to explain the highly selective gelation properties of compound 1, as is a comparison to non-gel forming structural isomer 2, which contains the same structural elements in a different arrangement. Finally, the ability of the gel derived from compound 1 to act as a reusable material for the efficient removal of cationic organic dyes from contaminated aqueous environments is also reported, with up to 11 repeated uses of the gel still maintaining the ability to effectively remove Rhodamine B.

show abstract

Self-assembly of pentapeptides in ethanol to develop organogels

Abstract: organogels have a wide variety of applications in the fields of chemistry, electricity, biomedicine and environmental engineering, which call for robust strategies for designing and developing novel organogelators. Here, we...

Cited by 10 publications

References 65 publications

Mapping of individual sensory nerve axons from digits to spinal cord with the Transparent Embedding Solvent System

Mapping of individual sensory nerve axons from digits to spinal cord with the Transparent Embedding Solvent System

Gelation of a Pentapeptide in Alcohols

Sonication-Induced, Solvent-Selective Gelation of a 1,8-Napthalimide-Conjugated Amide: Structural Insights and Pollutant Removal Applications

Contact Info

Product

Resources

About