Self-assembly of nucleic acid molecular aggregates catalyzed by a triple-helix probe for miRNA detection and single cell imaging

Zhang, Zhen; Wang, Yuanyuan; Zhang, Ningbo; Zhang, Shusheng

doi:10.1039/c6sc00694a

Cited by 73 publications

(47 citation statements)

References 38 publications

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“…The fluorescence intensity has a linear correlation with the logarithm of let-7d concentration from 10 −13 to 10 −9 M. The standard curve equation is F’ = 10462 + 751 logC with a correlation coefficient of 0.9971, where C and F’ are the concentration of let-7d and the fluorescence intensity (F’ = F-F0), respectively. The detection limit is calculated to be 1.5 × 10 −13 M, according to the 3σ rule, which is much lower than that of the existing signal amplification in our previous report34. Moreover, the result was comparable with other amplification methods and the comparison of different methods for miRNA detection is shown in Table S1.…”

Section: Resultsmentioning

confidence: 48%

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Zheng

Niu

Wei

et al. 2016

Sci Rep

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MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to initiate strand displacement amplification (SDA) in tandem. The released single strand DNA will be a primer to bind the circular template to initiate rolling circle amplification (RCA). The products based on multiple amplifications will be detected by a standard fluorimeter with N-methyl mesoporphyrin IX (NMM) as the fluorescent indicator. The proposed method exhibited excellent selectivity and high sensitivity with a detection limit of as low as 1.5 × 10−13 M. Moreover, this methodology was used for the determination of biomolecules in real serum samples with satisfying results.

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Section: Resultsmentioning

confidence: 48%

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Zheng

Niu

Wei

et al. 2016

Sci Rep

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“…F‐PNA could induce fluorescence quenching but F‐PNA/RCAP could attenuate the quenching of F‐PNA fluorescence due to adsorption and desorption on the surface of GO. Zhang and co‐workers also used this powerful method to design a system that could clearly distinguish cancer cells from normal cells . To realize naked eye detection, Zhu et al developed a new RGO‐assisted RCA amplification strategy to detect miRNA single nucleotide polymorphisms (SNPs); miR‐125a, let‐7a, their single base mutants and other specific mutants were adopted as models for this study, as shown in Figure B.…”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

“…Zhang and co-workers also used this powerful method to design a system that could clearly distinguish cancer cells from normal cells. [119] To realize naked eye detection, Zhu et al [120] developed a new RGO-assisted RCA amplification strategy to detect miRNA single nucleotide polymorphisms (SNPs); miR-125a, let-7a, their single base mutants and other specific mutants were adopted as models for this study, as shown in Figure 8B. Compared with the traditional PRC technology, the selectivity of RGO-assisted RCA was greatly improved, and the miRNA SNP signal was as high as 100-fold that of the control signal.…”

Section: Fluorescence Detection With Enzyme Participationmentioning

confidence: 99%

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Zhang

Miao

Sun

et al. 2019

Small

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MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs that play critical roles in physiologic and pathologic processes and are vital biomarkers for several disease diagnostics and therapeutics. Therefore, rapid, low‐cost, sensitive, and selective detection of miRNAs is of paramount importance and has aroused increasing attention in the field of medical research. Among the various reported miRNA sensors, devices based on graphene and its derivatives, which form functional supramolecular nanoassemblies of π‐conjugated molecules, have been revealed to have great potential due to their extraordinary electrical, chemical, optical, mechanical, and structural properties. This Review critically and comprehensively summarizes the recent progress in miRNA detection based on graphene and its derivative materials, with an emphasis on i) the underlying working principles of these types of sensors, and the unique roles and advantages of graphene materials; ii) state‐of‐the‐art protocols recently developed for high‐performance miRNA sensing, including representative examples; and iii) perspectives and current challenges for graphene sensors. This Review intends to provide readers with a deep understanding of the design and future of miRNA detection devices.

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“…[40] In RT-PCR, atarget RNAofinterest is first reverse transcribed into complementary DNA(cDNA) transcripts by reverse transcriptase.T hen, the newly-produced cDNAi s amplified through traditional PCR in the presence of thermostable polymerase,r esulting in indirect amplification-based detection of the target RNA. [44][45][46][47][48][49][50][51][52][53] In FISH, enzymes that can catalyze the amplification of nucleic acid under ambient temperature are generally transferred into cells,t hen the target nucleic acid of interest is amplified, leading to af luorescence signal. [41][42][43] In addition, isothermal enzymatic signal amplificationbased fluorescence in situ hybridization (FISH) assay has been developed for single-cell RNAi maging.…”

Section: Traditional Amplification-based Detection Of Rnamentioning

confidence: 99%

In Situ Amplification‐Based Imaging of RNA in Living Cells

Qing

et al. 2019

Angewandte Chemie

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Owing to its important physiological functions, especially as molecular biomarkers of diseases, RNA is an important focus of biomedicine and biochemical sensing. Signal amplification detection has been put forward because of the need for accurate identification of RNA at low expression levels, which is significant for the early diagnosis and therapy of malignant diseases. However, conventional amplification methods for RNA analysis depend on the use of enzymes, fixation of cells, and thermal cycling, which confine their performance to cell lysates or dead cells, thus the imaging of RNA in living cells remained until recently little explored. In recent years, the advance of isothermal amplification of nucleic acids has opened paths for meeting this need in living cells. This minireview tracks the development of in situ amplification assays for RNAs in living cells, and highlights the potential challenges facing this field, aiming to improve the development of in vivo isothermal amplification as well as usher in new frontiers in this fertile research area.

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Self-assembly of nucleic acid molecular aggregates catalyzed by a triple-helix probe for miRNA detection and single cell imaging

Abstract: We developed a new and highly sensitive method for miRNA detection and intracellular imaging based on novel nucleic acid molecular aggregates self-assembled on graphene oxide nanoplates.

Cited by 73 publications

References 38 publications

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

Progress in miRNA Detection Using Graphene Material–Based Biosensors

In Situ Amplification‐Based Imaging of RNA in Living Cells

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