Self‐assembly of multiscale anisotropic hydrogels through interfacial polyionic complexation

Patel, Akhil; Sant, Vinayak; Velankar, Sachin; Dutta, Mayuri; Balasubramanian, Vibishan; Sane, P. P.; Agrawal, Vishi; Wilson, J. E.; Rohan, Lisa C.; Sant, Shilpa

doi:10.1002/jbm.a.37001

Cited by 11 publications

(12 citation statements)

References 46 publications

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“…Moreover, the RegenMatrix hydrogel used in this study showed 674.3% (±103.4) elongation and 29.6 (±11.8) kJ/m 3 toughness when tested as a bilayer film in our previous study. 14 Swelling studies of the bilayer film hydrogels showed 417.5% (±125.4) equilibrium swelling compared to dry bilayer films (data not shown).…”

Section: ■ Results and Discussionmentioning

confidence: 94%

“…CHT (1% w/v) and KCA (1% w/v) solutions showed +82 (±4.78) and negative −79.5 (±4.21) zeta potential as reported in our previous study. 14 Two programmable syringe pumps (BS300, Braintree Scientific INC., Braintree, MA) were used at a speed of 50 mL/h. Each pump held CHT (1% w/v) and KCA (1% w/v) solutions in separate 10 mL plastic syringes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The water-rich microenvironment of hydrogels makes them ideal biomaterials mimicking the native ECM . While most studies utilize hydrogels as a carrier material to deliver highly potent drug or growth factors locally, − their ability to recreate tissue microarchitecture, multiscale hierarchy − and thereby, recreate a favorable biomimetic microenvironment to harness their regenerative potential remain underutilized. Albeit less potent than added growth factors, these biophysical properties stimulate favorable cellular activity to repair and regenerate the damaged tissue .…”

Section: Introductionmentioning

confidence: 99%

“…Here, we propose ECM-mimetic bioactive materials without added growth factors that can guide and enhance tissue regeneration through the mimicry of the multiscale architecture of bone ECM and native collagen. ,, Indeed, native collagen molecules self-assemble into triple helices, fibrils, fibers, and fiber bundles. At nanoscale, native collagen also exhibits a periodic pattern of dark/light bands that contributes to orderly mineral deposition .…”

Section: Introductionmentioning

confidence: 99%

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Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors

Patel

Zaky

et al. 2020

ACS Appl. Bio Mater.

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Hydrogel-based biomaterials have advanced bone tissue engineering approaches in the last decade, through their ability to serve as a carrier for potent growth factor, bone morphogenic protein-2 (BMP-2). However, biophysical properties of hydrogels such as multiscale structural hierarchy and bone extracellular matrix (ECM)-mimetic microarchitecture are underutilized while designing current bone grafts. Incorporation of these properties offers great potential to create a favorable biomimetic microenvironment to harness their regenerative potential. Here, we present our approach to fabricate collagen-inspired bioactive hydrogel scaffolds (referred to as “RegenMatrix”) to guide and enhance bone regeneration in a rabbit ulna defect model through the mimicry of multiscale architecture of bone ECM, i.e., native collagen. Specifically, we employed polyelectrolyte complexation to promote bottom-up self-assembly of oppositely charged polysaccharides (chitosan and kappa-carrageenan) at multiple length scales forming fibrils, which further assemble into fibers. The self-assembly and bioinspired scaffold fabrication method resulted in robust cylindrical RegenMatrix with excellent retention of the multiscale architecture and uniform mineral deposition throughout the scaffolds. RegenMatrix, in both nonmineralized and mineralized forms, enhanced bone regeneration in the semiload-bearing ulna defect when compared to the empty defect. RegenMatrix also showed greater histocompatibility without any fibrous tissue formation. Collectively, the RegenMatrix developed in this study has a great potential as a bioactive bone graft without any added growth factors.

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Section: ■ Results and Discussionmentioning

confidence: 94%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors

Patel

Zaky

et al. 2020

ACS Appl. Bio Mater.

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“…Interfacial polyionic complexation of oppositely charged polysaccharide pairs (i.e., positively charged CS with negatively charged κ-carr, alginate, or gellan gum) was used to produce continuous interfacial fibers with strong mechanical properties [269]. These bioinspired hydrogels presented multiscale hierarchy (parallel or perpendicular align-ment), ability to encapsulate NPs, small molecules or to form composites with other macromolecules [270,271].…”

Section: Other Polyssacharidesmentioning

confidence: 99%

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Bercea¹

2022

Polymers

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Hydrogels, as interconnected networks (polymer mesh; physically, chemically, or dynamic crosslinked networks) incorporating a high amount of water, present structural characteristics similar to soft natural tissue. They enable the diffusion of different molecules (ions, drugs, and grow factors) and have the ability to take over the action of external factors. Their nature provides a wide variety of raw materials and inspiration for functional soft matter obtained by complex mechanisms and hierarchical self-assembly. Over the last decade, many studies focused on developing innovative and high-performance materials, with new or improved functions, by mimicking biological structures at different length scales. Hydrogels with natural or synthetic origin can be engineered as bulk materials, micro- or nanoparticles, patches, membranes, supramolecular pathways, bio-inks, etc. The specific features of hydrogels make them suitable for a wide variety of applications, including tissue engineering scaffolds (repair/regeneration), wound healing, drug delivery carriers, bio-inks, soft robotics, sensors, actuators, catalysis, food safety, and hygiene products. This review is focused on recent advances in the field of bioinspired hydrogels that can serve as platforms for life-science applications. A brief outlook on the actual trends and future directions is also presented.

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Nanocomposite Hydrogels in Regenerative Medicine: Applications and Challenges

Chen

Yue

Wang

et al. 2023

Macromol. Rapid Commun.

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Regenerative medicine is a highly regarded multidisciplinary field that aims to transform the future of clinical medicine through curative strategies rather than palliative therapies. As an emerging field, the development of regenerative medicine cannot be achieved without multifunctional biomaterials. Among the various bioscaffold materials, hydrogels are one of the materials of interest in bioengineering and medical research because of their similarity to the natural extracellular matrix and good biocompatibility. However, conventional hydrogels have simple internal structures and single cross‐linking modes, which require improvement in a single function and structural stability. Introducing multifunctional nanomaterials into 3D hydrogel networks physically or chemically avoids their disadvantages. Nanomaterials (NMs) are materials in the size range of 1–100 nm with distinct physical and chemical properties that differ from that of the macroscopic size and enable hydrogels to exhibit multifunctionality. Although regenerative medicine and hydrogels have been well researched in their respective fields, the connection between nanocomposite hydrogels (NCHs) and regenerative medicine has not been elaborated. Therefore, this review briefly describes the preparation and design requirements of NCHs and discusses their applications and challenges in regenerative medicine, hoping to clarify the relationship between the two.

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Self‐assembly of multiscale anisotropic hydrogels through interfacial polyionic complexation

Cited by 11 publications

References 46 publications

Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors

Bottom-Up Self-assembled Hydrogel-Mineral Composites Regenerate Rabbit Ulna Defect without Added Growth Factors

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Nanocomposite Hydrogels in Regenerative Medicine: Applications and Challenges

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