Self-Assembly of Hierarchical DNA Nanotube Architectures with Well-Defined Geometries

Abstract

An essential motif for the assembly of biological materials such as actin at the scale of hundreds of nanometers and beyond is a network of one-dimensional fibers with well-defined geometry. Here, we demonstrate the programmed organization of DNA filaments into micron-scale architectures where component filaments are oriented at preprogrammed angles. We assemble L-, T-, and Y-shaped DNA origami junctions that nucleate two or three micron length DNA nanotubes at high yields. The angles between the nanotubes mirror the angles between the templates on the junctions, demonstrating that nanoscale structures can control precisely how micron-scale architectures form. The ability to precisely program filament orientation could allow the assembly of complex filament architectures in two and three dimensions, including circuit structures, bundles, and extended materials.

Self-assembly as a key player for materials nanoarchitectonics

“…One of the typical successful examples would be DNA nanotechnology such as DNA origami in which various structures can be logically nanoarchitected through specific baseparing and programmed DNA sequences [200][201][202]. Schulman and co-workers demonstrated hierarchic self-assembly of DNA nanotubes in micrometre scale from DNA strands with programmed sequences [203]. Even though the assembled structures expanded into micrometre scales, their geometry can be decided by smaller scale DNA origami junctions.…”

A review of the interfacial characteristics of polymer nanocomposites containing carbon nanotubes

“…190 They also affected the properties of the nal composite, 191 especially mechanical properties. Uniform dispersion of carbon nanotubes within a DNA matrix can be achieved, 192 and their complexes have been found to enable separation of carbon nanotubes 190,193 as well as preparation of composites and bers [194][195][196] and nanometer-scale electronic (nanoelectronic) devices. 197,198 An in-depth review of the science and technology of carbon nanotube-DNA composite materials and related applications is available in the literature.…”

DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy

“…DNA cages refer to wireframe architectures assembly from DNA strands (Chen and Seeman, 1991;Wang et al, 2019). Various DNA cages include DNA polyhedrons (Wang et al, 2019) and DNA nanotube (Jorgenson et al, 2017;Mohammed et al, 2017) were reported. DNA polyhedrons represent a 3D cage-like compact structure which is stable and easily absorbed by cells.…”

“…DNA polyhedrons represent a 3D cage-like compact structure which is stable and easily absorbed by cells. Due to structural closure, they are compact, mechanically strong, size-tunable and noncytotoxic (Jorgenson et al, 2017;Mohammed et al, 2017). Among them, DNA tetrahedron is the most commonly seen one (He et al, 2008;Liang et al, 2014), as demonstrated in Figure 1.…”