Self-assembly of gold nanoparticles grafted with amphiphilic supramolecular block copolymers

Wei, Zichao; Liu, Chung‐Hao; Duan, Hanyi; Luo, Qiang; Huang, Margaret; Thanneeru, Srinivas; Nieh, Mu‐Ping; He, Jie

doi:10.1016/j.giant.2022.100102

Cited by 7 publications

(5 citation statements)

References 70 publications

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“…UV-vis spectroscopy showed that the AuNPs exhibited absorption peaks within the accepted range for gold. The FAuNPs had a lower peak intensity, suggesting that the respective polymers coated the gold [35]. FTIR analysis is commonly used to detect functional groups and further confirmed the AuNP synthesis and functionalization.…”

Section: Discussionmentioning

confidence: 72%

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Zenze,

Singh

2024

IJMS

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The formulation of novel delivery protocols for the targeted delivery of genes into hepatocytes by receptor mediation is important for the treatment of liver-specific disorders, including cancer. Non-viral delivery methods have been extensively studied for gene therapy. Gold nanoparticles (AuNPs) have gained attention in nanomedicine due to their biocompatibility. In this study, AuNPs were synthesized and coated with polymers: chitosan (CS), and polyethylene glycol (PEG). The targeting moiety, lactobionic acid (LA), was added for hepatocyte-specific delivery. Physicochemical characterization revealed that all nano-formulations were spherical and monodispersed, with hydrodynamic sizes between 70 and 250 nm. Nanocomplexes with pCMV-Luc DNA (pDNA) confirmed that the NPs could bind, compact, and protect the pDNA from nuclease degradation. Cytotoxicity studies revealed that the AuNPs were well tolerated (cell viabilities > 70%) in human hepatocellular carcinoma (HepG2), embryonic kidney (HEK293), and colorectal adenocarcinoma (Caco-2) cells, with enhanced transgene activity in all cells. The inclusion of LA in the NP formulation was notable in the HepG2 cells, which overexpress the asialoglycoprotein receptor on their cell surface. A five-fold increase in luciferase gene expression was evident for the LA-targeted AuNPs compared to the non-targeted AuNPs. These AuNPs have shown potential as safe and suitable targeted delivery vehicles for liver-directed gene therapy.

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Section: Discussionmentioning

confidence: 72%

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Zenze,

Singh

2024

IJMS

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“…The characteristic peak at B650 nm corresponds to the d-d transition of Cu 2+ ions bound with the N-containing ligand, i.e., DPA. 47,61,62 The peak intensity continuously increased with increasing the concentration of Cu(NO 3 ) 2 and then saturated with a red shift to 650 nm. The absorbance at 650 nm was plotted against the molar ratio of Cu 2+ -To-DPA, as shown in Fig.…”

Section: Resultsmentioning

confidence: 97%

“…, DPA. 47,61,62 The peak intensity continuously increased with increasing the concentration of Cu(NO 3 ) 2 and then saturated with a red shift to 650 nm. The absorbance at 650 nm was plotted against the molar ratio of Cu 2+ -To-DPA, as shown in Fig.…”

Section: Resultsmentioning

confidence: 97%

Hydrophobic pockets built in polymer micelles enhance the reactivity of Cu²⁺ions

Wei

Liu

Luo

et al. 2023

Mater. Chem. Front.

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“…As a reference, AgNPs grafted by PS 134 -SH was achieved by a single-phase ligand exchange. , Typically, 5 mg of PS 134 -SH was dissolved in 5 mL of DMF. Then, 5 mL of citrate-capped AgNPs was concentrated to 100 μL by centrifuging the solution at 8500 rpm for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Polymer N-Heterocyclic Carbene (NHC) Ligands for Silver Nanoparticles

Wei

Mullaj

Price

et al. 2022

ACS Appl. Mater. Interfaces

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Polymer N-heterocyclic carbenes (NHCs) are a class of robust surface ligands to provide superior colloidal stability for metal nanoparticles (NPs) under various harsh conditions. We report a general method to prepare polymeric NHCs and demonstrate that these polymer NHC–AgNPs are stable against oxidative etching and show high peroxidase activity. We prepared three imidazolium-terminated poly(methyl methacrylate) (PMMA), polystyrene (PS), and poly(2-(2-methoxyethoxy)ethyl methacrylate) (PMEO2MA) through atom-transfer radical polymerization with an imidazole-containing initiator. The imidazolium end group was further converted to NHC–Ag(I) in the presence of Ag2O at room temperature. Polymer NHC–Ag(I) can transmetalate to AgNPs through ligand exchange at the interface of oil/water within 2 min. All the three polymers can modify metal NPs, such as AgNPs, Ag nanowires, and AuNPs, providing excellent thermal, oxidative, and chemical stabilities for AgNPs. As an example, in the presence of hydrogen peroxide, AgNPs modified by polymer NHCs were resistant against oxidative etching with a rate of ∼700 times slower than those grafted with thiolates. AgNPs modified by polymer NHCs also showed higher peroxidase activity, 4 times more active than those capped by citrate and polyvinylpyrrolidone (PVP) and 2 times more active than those with polymer thiolate. Our studies demonstrate a great potential of using polymer NHCs to stabilize metallic NPs for various applications.

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Self-assembly of gold nanoparticles grafted with amphiphilic supramolecular block copolymers

Cited by 7 publications

References 70 publications

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Hydrophobic pockets built in polymer micelles enhance the reactivity of Cu²⁺ions

Polymer N-Heterocyclic Carbene (NHC) Ligands for Silver Nanoparticles

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Self-assembly of gold nanoparticles grafted with amphiphilic supramolecular block copolymers

Cited by 7 publications

References 70 publications

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

Hydrophobic pockets built in polymer micelles enhance the reactivity of Cu2+ions

Polymer N-Heterocyclic Carbene (NHC) Ligands for Silver Nanoparticles

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Hydrophobic pockets built in polymer micelles enhance the reactivity of Cu²⁺ions