Self‐Assembly of Fibers and Nanorings from Disulfide‐Linked Helix–Loop–Helix Polypeptides

Aili, Daniel; Tai, Feng-I; Enander, Karin; Baltzer, Lars; Liedberg, Bo

doi:10.1002/anie.200801155

Cited by 30 publications

(40 citation statements)

References 28 publications

(34 reference statements)

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“…[7][8][9][10][11][12][13] Furthermore, by connecting two or more peptide using short linkers, fairly complex assembled structures can be formed as result of the well-defined folding pattern of each single peptide folding motif. [14][15][16][17][18] Peptides have also been conjugated to larger synthetic polymeric backbones, such as poly(ethylene glycol) (PEG), in order to create a wider range of supramolecular hybrid materials and nanostructures, [19][20][21] including peptide-polymer hybrid hydrogels, fibrils, spherulities and micells. [21][22][23][24][25][26] PEG is an attractive component in hydrogels and have been extensively investigated for 3D cell culture, and is used in a large number of clinical and pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Tailoring Supramolecular Peptide–Poly(ethylene glycol) Hydrogels by Coiled Coil Self-Assembly and Self-Sorting

2016

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Physical hydrogels are extensively used in a wide range of biomedical applications. However, different applications require hydrogels with different mechanical and structural properties.Tailoring these properties demands exquisite control over the supramolecular interactions involved. Here we show that it is possible to control the mechanical properties of hydrogels using de novo designed coiled coil peptides with different affinities for dimerization. Four different non-orthogonal peptides, designed to fold into four different coiled coil heterodimers with dissociation constants spanning from µM to pM, were conjugated to star-shaped 4-armpoly(ethylene glycol) (PEG). The different PEG-coiled coil conjugates self-assemble as a result of peptide heterodimerization. Different combinations of PEG-peptide conjugates assemble into PEG-peptide networks and hydrogels with distinctly different thermal stabilities, supramolecular and rheological properties, reflecting the peptide dimer affinities. We also demonstrate that it is possible to rationally modulate the self-assembly process by means of thermodynamic self-sorting by sequential additions of non-pegylated peptides. The specific interactions involved in peptide dimerization thus provides means for programmable and reversible self-assembly of hydrogels with precise control over rheological properties, which 2 can significantly facilitate optimization of their overall performance and adaption to different processing requirements and applications.

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Section: Introductionmentioning

confidence: 99%

Tailoring Supramolecular Peptide–Poly(ethylene glycol) Hydrogels by Coiled Coil Self-Assembly and Self-Sorting

2016

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“…The 42 amino acid residue helix-loop-helix peptide JR2EC is designed to fold and homodimerize into four-helical bundles at acidic pH, 43 and at neutral pH in the presence of mM concentrations of Zn…”

Section: Resultsmentioning

confidence: 99%

Zinc-Triggered Hierarchical Self-Assembly of Fibrous Helix–Loop–Helix Peptide Superstructures for Controlled Encapsulation and Release

2016

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We demonstrate a novel route for hierarchical self-assembly of sub-micron sized peptide superstructures that respond to subtle changes in Zn 2+ concentration. The self-assembly process is triggered by a specific foldingdependent coordination of Zn 2+ by a de novo designed non-linear helix-loop-helix peptide, resulting in a propagating fiber formation and formation of spherical superstructures. The superstructures further forms larger assemblies that can be completely disassembled upon removal of Zn 2+ or degradation of the non-linear peptide.This flexible and reversible assembly strategy of the superstructures enable facile encapsulation of nanoparticles and drugs that can be released by means of different stimuli.

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“…When oxidized, these peptides self-assemble into fibrous nanostructures by a folding-mediated heteroassociation process. [14] Immobilization of the Glu rich 25 polypeptide JR2EC on spherical gold nanoparticles yields highly stable dispersions. Addition of oxidized JR2KC (JR2KC 2 ) to JR2EC modified particles results in a folding dependent bridging aggregation, caused by the heteroassociation and folding of the peptides into disulphide-linked four-helix bundles that span in 30 between the particles.…”

Section: Peptide Functionalizationmentioning

confidence: 99%

Specific functionalization of CTAB stabilized anisotropic gold nanoparticles with polypeptides for folding-mediated self-assembly

et al. 2012

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Anisotropic nanoparticles stabilized by cetyltrimethylammonium bromide (CTAB) are notoriously difficult to homogenously functionalize using conventional gold-thiol chemistry. Using surface assisted laser desorption time of flight mass spectroscopy and scanning transmission electron microscopy-energy dispersive X-ray spectroscopy, we demonstrate that silver species adsorbed on the particle surface prevent 10 effective surface functionalization. When covered by a thin gold film, particle functionalization was drastically improved. A thiol-containing polypeptide was immobilized on arrowhead gold nanorods (NRs) and was subsequently able to selectively heteroassociate with a complementary polypeptide resulting in a folding-mediated bridging aggregation of the NRs. Despite using arrowhead NRs with a pronounced difference in surface arrangement on the {111} facets on the arrowheads compared to the 15 {100} facets at the particle sides, the polypeptides were efficiently and homogeneously immobilized on the particles after gold film overgrowth.

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Self‐Assembly of Fibers and Nanorings from Disulfide‐Linked Helix–Loop–Helix Polypeptides

Cited by 30 publications

References 28 publications

Tailoring Supramolecular Peptide–Poly(ethylene glycol) Hydrogels by Coiled Coil Self-Assembly and Self-Sorting

Tailoring Supramolecular Peptide–Poly(ethylene glycol) Hydrogels by Coiled Coil Self-Assembly and Self-Sorting

Zinc-Triggered Hierarchical Self-Assembly of Fibrous Helix–Loop–Helix Peptide Superstructures for Controlled Encapsulation and Release

Specific functionalization of CTAB stabilized anisotropic gold nanoparticles with polypeptides for folding-mediated self-assembly

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