“…As in nature, synthetic assemblies comprised of biological macromolecules can be constructed through highly selective biomolecular interactions (previous references and Padilla et al, 2001;Smith et al, 1997), with the streptavidin-biotin system used most broadly (Moll et al, 2002;Niemeyer, 2001). However, the rigid and nearly covalent binding between streptavidin and biotin, and the inherently non-selective protein biotinylation that results in non-uniform complexation (Niemeyer et al, 1999) limit the design of responsive, smart nanomaterials. Conversely, TF-TFBS recognition is highly controllable, and therefore ideal to serve as the first level of a multicomponent assembly.…”