Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins

Varjonen, Suvi; Laaksonen, Päivi; Paananen, Arja; Valo, Hanna; Hähl, Hendrik; Laaksonen, Timo; Linder, Markus B.

doi:10.1039/c0sm01114b

Cited by 67 publications

(57 citation statements)

References 47 publications

(53 reference statements)

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“…c) Facilitated assembly of CNF at air/water interface via amphiphilic biomolecules and film transfer on a hydrophobic surface, d) AFM topography image of a self‐assembled protein/CNF film picked up from air/water interface, and e) AFM topography image of a self‐assembled protein/CNC film picked up from air/water interface. Reproduced with permission . Copyright 2011, Royal Society of Chemistry.…”

Section: Nanocelluloses As Colloidal‐level Structural Unitsmentioning

confidence: 99%

Advanced Materials through Assembly of Nanocelluloses

et al. 2018

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There is an emerging quest for lightweight materials with excellent mechanical properties and economic production, while still being sustainable and functionalizable. They could form the basis of the future bioeconomy for energy and material efficiency. Cellulose has long been recognized as an abundant polymer. Modified celluloses were, in fact, among the first polymers used in technical applications; however, they were later replaced by petroleum-based synthetic polymers. Currently, there is a resurgence of interest to utilize renewable resources, where cellulose is foreseen to make again a major impact, this time in the development of advanced materials. This is because of its availability and properties, as well as economic and sustainable production. Among cellulose-based structures, cellulose nanofibrils and nanocrystals display nanoscale lateral dimensions and lengths ranging from nanometers to micrometers. Their excellent mechanical properties are, in part, due to their crystalline assembly via hydrogen bonds. Owing to their abundant surface hydroxyl groups, they can be easily modified with nanoparticles, (bio)polymers, inorganics, or nanocarbons to form functional fibers, films, bulk matter, and porous aerogels and foams. Here, some of the recent progress in the development of advanced materials within this rapidly growing field is reviewed.

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Section: Nanocelluloses As Colloidal‐level Structural Unitsmentioning

confidence: 99%

Advanced Materials through Assembly of Nanocelluloses

et al. 2018

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“…19 Hydrophobins' ability to adhere to surfaces and to bind water is similar to that of lubricating glycoproteins, such as mucin and lubricin, which has motivated research into hydrophobins as potential water based lubricant additives. 12,14,20 Several studies on class II hydrophobins, such as HFBI and HFBII, have shown strong adhesion not only to hydrophobic interfaces 21,22 but also to hydrophilic, polarised surfaces. 23,24 The ability to form well defined protein monolayers can be beneficial when attempting to enhance the lubrication of materials such as stainless steel in water.…”

Section: Introductionmentioning

confidence: 99%

Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

Hakala

Laaksonen

Helle

et al. 2014

Tribology - Materials, Surfaces & Interfaces

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In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.

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“…Due to their globular and stable conformation and small size, class II hydrophobins have been employed in formation of welldefined layers at various interfaces [17][18][19]. By genetic engineering, additional functionalities can be brought to the protein layers by fusing the anchoring group with a second functional domain allowing functionalization of such interfacial layer [20,21]. However, only few studies describe the use of this interesting group of self-assembling proteins for the controlled nucleation-and-growth of inorganic materials [22].…”

Section: Introductionmentioning

confidence: 99%

Engineered Hydrophobin for Biomimetic Mineralization of Functional Calcium Carbonate Microparticles

Heinonen¹,

Laaksonen²,

Hentze³

2014

JBNB

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In this study, the modified hydrophobin, engineered for biomimetic mineralization, has been employed as a structure-directing agent for mineralization of calcium carbonate. For the first time amphiphilic calcium carbonate particles have been obtained, using engineered proteins. The mineral microparticles have been characterized by optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). While mineralization in the presence of non-modified hydrophobin results in polymorph mineral structures, uniform microspheres with an average particle diameter of one micron are obtained by employing hydrophobin which has been modified with an additional ceramophilic protein sequence. Owing to the tri-functionality of the modified hydrophobin (hydrophilic, hydrophobic and ceramophilic), the obtained mineral microparticles exhibit amphiphilic properties. Potential applications are in the areas of functional fillers and pigments, like biomedical and composite materials. Pickering emulsions have been prepared as a demonstration of the emulsion-stabilizing properties of the obtained amphiphilic mineral microspheres. The structure-directing effects of the studied engineered hydrophobins are compared with those of synthetic polymers (i.e. polycarboxylates) used as crystallization and scaling inhibitors in industrial applications.

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Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins

Cited by 67 publications

References 47 publications

Advanced Materials through Assembly of Nanocelluloses

Advanced Materials through Assembly of Nanocelluloses

Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

Engineered Hydrophobin for Biomimetic Mineralization of Functional Calcium Carbonate Microparticles

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