Self‐assembly multifunctional DNA tetrahedron for efficient elimination of antibiotic‐resistant bacteria

Abstract

Antibiotic resistance is a major challenge in the clinical treatment of bacterial infectious diseases. Herein, we constructed a multifunctional DNA nanoplatform as a versatile carrier for bacteria‐specific delivery of clinical antibiotic ciprofloxacin (CIP) and classic nanoantibiotic silver nanoparticles (AgNP). In our rational design, CIP was efficiently loaded in the self‐assembly double‐bundle DNA tetrahedron through intercalation with DNA duplex, and single‐strand DNA‐modified AgNP was embedded in the cavity of the DNA tetrahedron through hybridization. With the site‐specific assembly of targeting aptamer in the well‐defined DNA tetrahedron, the bacteria‐specific dual‐antibiotic delivery system exhibited excellent combined bactericidal properties. With enhanced antibiotic accumulation through breaking the out membrane of bacteria, the antibiotic delivery system effectively inhibited biofilm formation and promoted the healing of infected wounds in vivo. This DNA‐based antibiotic delivery system provides a promising strategy for the treatment of antibiotic‐resistant infections.

Near-infrared light-heatable platinum nanozyme for synergistic bacterial inhibition

“…Many different new antimicrobial strategies have been reported in recent years ( Sun et al, 2020 ; Niu et al, 2021a ; Niu et al, 2021b ; Du et al, 2021 ; Zhang et al, 2021 ; Li et al, 2022a ; Li et al, 2022b ; Fu et al, 2022 ; Guo et al, 2022 ; Wang et al, 2023 ; Wu et al, 2023 ). Among them, nanomaterial-mediated antimicrobial strategies show more significant advantages, including easy material synthesis, being less susceptible to bacterial resistance, and a high bacterial killing efficiency.…”

Natural product nanozymes of herbal extract galangin in managing hepatocellular carcinoma

“…Galangin (3,5,7-trihydroxyflavone) is regarded as the bioactive constituent of galangal, a perennial plant native of Indonesia but cultivated in many parts of Asia. The anticancer effects of galangin are mostly due to its abilities to inhibit cell cycle progression, inhibiting mitogen-activated protein kinase (MAPK) (Liu et al, 2015), protein kinase B (Akt) (Li et al, 2018), or mammalian target of rapamycin (mTOR) activity thereby inducing apoptotic cell death by activating caspase-9/-8/-3 and inhibiting tumor invasion and metastasis by reducing the upregulation of matrix metalloproteinase-2/-9 (Kong et al, 2019; Frontiers in Chemistry frontiersin.org Wu et al, 2024). These molecular pathways regulated by galangin are involved in suppressing different malignancies, such as lung cancer, hepatic cancer, ovarian cancer, breast cancer, gastric cancer, colorectal cancer, retinoblastoma, and osteosarcoma (Liang et al, 2021).…”

DataSheet1.docx

“…Galangin (3,5,7-trihydroxyflavone) is regarded as the bioactive constituent of galangal, a perennial plant native of Indonesia but cultivated in many parts of Asia. The anticancer effects of galangin are mostly due to its abilities to inhibit cell cycle progression, inhibiting mitogen-activated protein kinase (MAPK) (Liu et al, 2015), protein kinase B (Akt) (Li et al, 2018), or mammalian target of rapamycin (mTOR) activity thereby inducing apoptotic cell death by activating caspase-9/-8/-3 and inhibiting tumor invasion and metastasis by reducing the upregulation of matrix metalloproteinase-2/-9 (Kong et al, 2019; Frontiers in Chemistry frontiersin.org Wu et al, 2024). These molecular pathways regulated by galangin are involved in suppressing different malignancies, such as lung cancer, hepatic cancer, ovarian cancer, breast cancer, gastric cancer, colorectal cancer, retinoblastoma, and osteosarcoma (Liang et al, 2021).…”