Three-dimensional networks of nanofibers, which are formed through self-assembly of peptide amphiphiles, serve as a biomimetic hydrogel scaffold for tissue engineering. With an emphasis to improve hydrogel properties for cell-specific behavior, a better understanding between structural characteristics and physical properties of the macroscopic gel is sought. Large-scale molecular dynamics simulations were performed on two PA sequences with identical composition (palmitoyl-V 3 A 3 E 3 and palmitoyl-A 3 V 3 E 3 ) showing different self-assembly kinetic mechanisms. While both sequences yielded cylindrical nanofibers, these structures have contrasting internal arrangement with respect to the hydrophobic core; the former is continuous with predominately alkyl tails, whereas the latter is disjointed with interconnecting micelles. Two additional sequences (palmitoyl-V 6 E 3 and palmitoyl-A 6 E 3 ) were examined to determine the effects of a homogeneous β-sheet forming segment that is either strongly or mildly hydrophobic on self-assembly. Results from this study indicate that internal structural arrangement of nanofibers can provide a correlation with structural stability and mechanical behavior of hydrogel nanostructures.