Self-assembling tetrameric peptides allow <i>in situ</i> 3D bioprinting under physiological conditions

Rauf, Sakandar; Susapto, Hepi Hari; Kahin, Kowther; Alshehri, Salwa; Abdelrahman, Sherin; Lam, Jordy Homing; Asad, Sultan; Jadhav, Sandip V.; Sundaramurthi, Dhakshinamoorthy; Gao, Xin; Hauser, Charlotte

doi:10.1039/d0tb02424d

Cited by 42 publications

(60 citation statements)

References 47 publications

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“…However, in the translation of synthetic peptide materials to bioinks, these key predictors are often under-reported and seemingly inconsistent. In reported studies of SAP bioinks, measures of printability such as viscosity [ 80 , 81 , 82 , 104 ], loss tangent [ 80 , 81 , 102 ], shear-thinning [ 81 , 82 , 103 ], achievable height [ 81 , 82 , 99 , 103 ] and filament assessments [ 81 , 82 , 103 ], were briefly discussed. This demonstrates the adoption of printability measures into the SAP bioink field; however, the lack of standard printability outcomes and the inconsistency in relationships of printability and predictors such as viscosity [ 81 , 82 ] limit the understanding of key material properties for future development.…”

Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

“…This has required the development of novel fabrication setups to enhance gelation. Bioprinting techniques amenable to SAP bioinks include droplet printing [ 98 , 99 , 100 , 101 ], or the generation of droplets which are then extruded [ 100 , 101 ], extrusion printing [ 80 , 81 , 102 , 103 , 104 ], and the customisation of extrusion printing setups, such as coaxial nozzles to mix salt solutions [ 80 , 81 ], printing onto salt-covered substrates [ 82 ], or removing excess fluid with a vacuum print-bed [ 105 ]. This demonstrates that the unique properties of SAP materials can be exploited for bioprinting.…”

Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

“…Progress into biomaterial molecular modelling and design principles may improve the clinical translation of materials by predicting outcomes without significant labour-intensive bench time. Molecular modelling [ 80 , 81 , 82 , 83 , 84 ], design principles [ 85 , 86 , 87 , 88 , 89 , 90 , 91 ] and predictive gelation models [ 90 ] of synthetic peptide materials are being increasingly reported, indicating a future ramp-up of high-throughput peptide biomaterial discovery. Synthetic self-assembling peptide (SAP) hydrogels are peptide sequences that self-assemble via supramolecular interactions to spontaneously immobilise fluid, creating a highly hydrated scaffold [ 92 ].…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic peptide materials give rise to complex biomimetic structures with bioactivity, resulting in controlled cell-scaffold interactions [ 96 , 97 ]. Furthermore, recent reports have demonstrated the translation of synthetic SAP biomaterials into bioinks [ 80 , 81 , 82 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ]. This demonstrates the potential of synthetic SAP design for biofabrication of organs and tissues, and ultimately clinical translation ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Peptide Biomaterials for Biofabrication

et al. 2021

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Biofabrication using well-matched cell/materials systems provides unprecedented opportunities for dealing with human health issues where disease or injury overtake the body’s native regenerative abilities. Such opportunities can be enhanced through the development of biomaterials with cues that appropriately influence embedded cells into forming functional tissues and organs. In this context, biomaterials’ reliance on rigid biofabrication techniques needs to support the incorporation of a hierarchical mimicry of local and bulk biological cues that mimic the key functional components of native extracellular matrix. Advances in synthetic self-assembling peptide biomaterials promise to produce reproducible mimics of tissue-specific structures and may go some way in overcoming batch inconsistency issues of naturally sourced materials. Recent work in this area has demonstrated biofabrication with self-assembling peptide biomaterials with unique biofabrication technologies to support structural fidelity upon 3D patterning. The use of synthetic self-assembling peptide biomaterials is a growing field that has demonstrated applicability in dermal, intestinal, muscle, cancer and stem cell tissue engineering.

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Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancing Peptide Biomaterials for Biofabrication

et al. 2021

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“…Bioinks based on ultrashort peptides composed of 3-7 natural amino acids boast great potential due to their natural but synthetic properties and their salt-enhanced instantaneous gelation [6][7][8][9][10] . Unlike polymers or gelatin and alginate-based bioinks, these peptide bioinks eliminate harmful processes of UV light and chemical exposures for post-printed crosslinking of the bioinks.…”

Section: Introductionmentioning

confidence: 99%

Time-dependent pulsing of microfluidic pumps to enhance 3D bioprinting of peptide bioinks

Khan

Kahin

Hauser

2021

Microfluidics, BioMEMS, and Medical Microsystems XIX

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Using bioinks for 3D bioprinting of cellular constructs remains a challenge due to factors including viscosity, fluid dynamics and shear stress. The encapsulation of cells within the bioinks directly affects the quality of 3D bioprinting and microfluidic pumping is a commonly used supporting approach. The accuracy of microfluidic pumps can be further improved by introducing various mixing techniques. However, many of these techniques introduce complex geometries or external fields. In this study, we used a simple control technique of time-dependent pulsing for instant gelation of the peptide bioinks and observed its effect during the bioprinting process. Various time-dependent periodic signals are imposed on to a stable flow cycle and the effects are analyzed. The microfluidic pumps are programmed with different flow patterns represented by low frequency sinusoidal pulses, ramp inputs, and duty cycle pulses. Different combinations of these pulses are tested to achieve an optimal pulse for improved quality of printed constructs. Time-varied pulsing of microfluidic pumps, particularly as square waveforms, is found to provide better continuous flow and avoid material buildup within the extruder unit when compared to pumping at a constant flow rate with manual tuning. Clogging is avoided since the gelation rate is periodically reduced which avoids gel clumps in the printed constructs. This study substantially improves the use of suitable peptide bioinks, standardizes the 3D bioprinting process, and reduces clogging and clumping during printing. Our findings allow for printing of more accurate and complex constructs for applications in tissue engineering, such as skin grafting, and other regenerative medical applications.

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Bioinks and Bioprinting Strategies for Skeletal Muscle Tissue Engineering

et al. 2022

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Skeletal muscles play important roles in critical body functions and their injury or disease can lead to limitation of mobility and loss of independence. Current treatments result in variable functional recovery, while reconstructive surgery, as the gold-standard approach, is limited due to donor shortage, donor-site morbidity, and limited functional recovery. Skeletal muscle tissue engineering (SMTE) has generated enthusiasm as an alternative solution for treatment of injured tissue and serves as a functional disease model. Recently, bioprinting has emerged as a promising tool for recapitulating the complex and highly organized architecture of skeletal muscles at clinically relevant sizes. Here, skeletal muscle physiology, muscle regeneration following injury, and current treatments following muscle loss are discussed, and then bioprinting strategies implemented for SMTE are critically reviewed. Subsequently, recent advancements that have led to improvement of bioprinting strategies to construct large muscle structures, boost myogenesis in vitro and in vivo, and enhance tissue integration are discussed. Bioinks for muscle bioprinting, as an essential part of any bioprinting strategy, are discussed, and their benefits, limitations, and areas to be improved are highlighted. Finally, the directions the field should expand to make bioprinting strategies more translational and overcome the clinical unmet needs are discussed.

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Self-assembling tetrameric peptides allow in situ 3D bioprinting under physiological conditions

Abstract: Tetrameric peptide-based bioinks allow the printing of 3D cell-laden scaffolds under true physiological conditions avoiding harsh UV or chemical treatment.

Cited by 42 publications

References 47 publications

Enhancing Peptide Biomaterials for Biofabrication

Enhancing Peptide Biomaterials for Biofabrication

Time-dependent pulsing of microfluidic pumps to enhance 3D bioprinting of peptide bioinks

Bioinks and Bioprinting Strategies for Skeletal Muscle Tissue Engineering

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