Self-Assembling RNA Nanoparticle for Gene Expression Regulation in a Model System

Jedrzejczyk, Dominika; Chworoś, Arkadiusz

doi:10.1021/acssynbio.8b00319

Cited by 8 publications

(15 citation statements)

References 58 publications

(88 reference statements)

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“…In this paper, we describe AuNPs associated with structural RNA nanoparticles (tectoRNAs), carrying regulatory siRNA fragments. In the field of RNA architectonics, scientists take advantage of RNA structural properties to create three-dimensional nanostructures with specific design and topology. − As a result of lasting studies in this area, it became possible to design and synthesize stable RNA nanoparticles that can regulate the expression of a target gene. − Such RNA architectures proved to be outstanding candidates for modern therapeutics, as they can be controllably equipped with several regulatory fragments to target multiple loci or genes . Upon delivery, structural RNAs are processed by cellular endonuclease Dicer ,, and introduced into an RNA-induced silencing complex (RISC) that leads to RNAi.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles as Carriers for Functional RNA Nanostructures

2021

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Conjugates of gold nanoparticles and ribonucleic acid are particularly interesting for biological applications to serve as therapeutics or biosensors. In this paper we present, for the first time, a conjugate of gold nanoparticles and structural RNA (tectoRNA), which serves as a tool for gene expression regulation. The tectoRNA trimer was modified to facilitate the introduction of a thiol linker, which aids the formation of stable RNA:AuNP conjugates. We demonstrated that these complexes can penetrate cells, which were observed in TEM analysis and are effective in gene expression regulation evident in GFP expression studies with fluorescence methods. The presented compounds have the potential to become a new generation of therapeutics that utilize the power of self-assembling, biologically active RNAs and gold nanoparticles, with their diagnostically useful optical properties and biocompatibility advantages.

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Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles as Carriers for Functional RNA Nanostructures

2021

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“…It should not come as a surprise. Indeed, RNA molecules provide a key to cell regulation and RNA nanoparticles/nanoclusters can be used for gene expression regulation (Jedrzejczyk and Chworos 2019). The RNA-based technology, known as the RNA silencing, can protect the eukaryotic cells against viruses and transposons (e.g., (Yang et al 2007) and references therein).…”

Section: Introductionmentioning

confidence: 99%

Atomistic to continuum model for studying mechanical properties of RNA nanotubes

Badu

Prabhakar

Melnik

et al. 2020

Computer Methods in Biomechanics and Biomedical Engineering

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With rapid advancements in the emerging field of RNA nanotechnology, its current and potential applications, new important problems arise in our quest to better understand properties of RNA nanocomplexes. In this paper, our focus is on the modeling of RNA nanotubes which are important for many biological processes. These RNA complexes are also important for human beings, with their theurapeutical and biomedical applications discussed vigorously in the literature over the recent years. Here, we develop a continuum model of RNA nanotubes, originally obtained from self assembly of RNA building blocks in the molecular dynamics simulation. Based on the finite element method, we calculate the elastic properties of these nanostructures and provide a relationship between stress and strain induced in the RNA nanotube. We also analyze the variations in the displacement vector along the assembly axis for RNA nanotubes of different sizes. In particular, we show that oscillations in the amplitudes of strains and displacements significantly differ for such RNA nanotubes. These findings are discussed in the context of atomistic simulations and experimental results in this field.

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Section: Introduction: Definition Challenges and Breakthrough Of Rna ...mentioning

confidence: 99%

Thermostability, Tunability, and Tenacity of RNA as Rubbery Anionic Polymeric Materials in Nanotechnology and Nanomedicine—Specific Cancer Targeting with Undetectable Toxicity

Binzel

Burns

et al. 2021

Chem. Rev.

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RNA nanotechnology is the bottom-up self-assembly of nanometer-scale architectures, resembling LEGOs, composed mainly of RNA. The ideal building material should be (1) versatile and controllable in shape and stoichiometry, (2) spontaneously self-assemble, and (3) thermodynamically, chemically, and enzymatically stable with a long shelf life. RNA building blocks exhibit each of the above. RNA is a polynucleic acid, making it a polymer, and its negative-charge prevents nonspecific binding to negatively charged cell membranes. The thermostability makes it suitable for logic gates, resistive memory, sensor set-ups, and NEM devices. RNA can be designed and manipulated with a level of simplicity of DNA while displaying versatile structure and enzyme activity of proteins. RNA can fold into single-stranded loops or bulges to serve as mounting dovetails for intermolecular or domain interactions without external linking dowels. RNA nanoparticles display rubber- and amoeba-like properties and are stretchable and shrinkable through multiple repeats, leading to enhanced tumor targeting and fast renal excretion to reduce toxicities. It was predicted in 2014 that RNA would be the third milestone in pharmaceutical drug development. The recent approval of several RNA drugs and COVID-19 mRNA vaccines by FDA suggests that this milestone is being realized. Here, we review the unique properties of RNA nanotechnology, summarize its recent advancements, describe its distinct attributes inside or outside the body and discuss potential applications in nanotechnology, medicine, and material science.

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Self-Assembling RNA Nanoparticle for Gene Expression Regulation in a Model System

Cited by 8 publications

References 58 publications

Gold Nanoparticles as Carriers for Functional RNA Nanostructures

Gold Nanoparticles as Carriers for Functional RNA Nanostructures

Atomistic to continuum model for studying mechanical properties of RNA nanotubes

Thermostability, Tunability, and Tenacity of RNA as Rubbery Anionic Polymeric Materials in Nanotechnology and Nanomedicine—Specific Cancer Targeting with Undetectable Toxicity

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