Self-assembled ternary complexes of plasmid DNA, low molecular weight polyethylenimine and targeting peptide for nonviral gene delivery into neurons

Zeng, Jieming; Wang, Xu; Wang, Shu

doi:10.1016/j.biomaterials.2006.11.015

Cited by 54 publications

(38 citation statements)

References 29 publications

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“…The distribution of drugs delivered in this way is, however, limited due to CSF flow. In our previous studies [16,20,21], PEI/DNA complexes delivered through lumbar intrathecal injection resulted in gene expression mainly in the lumbar and thoracic spinal cord. This distribution was not improved even using polyethylene glycolgrafted PEI/DNA complexes [20].…”

Section: Discussionmentioning

confidence: 99%

“…We have previously developed a method to prepare ternary complexes of low molecular weight PEI, plasmid DNA and a peptide derived from the nerve growth factor for targeted neuronal gene delivery [16], which demonstrates that it is crucial to apply cationic polymers first to condense plasmid DNA to an extent that some of negatively charged DNA molecules are still exposed on the surface for peptide binding. After using PolyMag and the Tat peptide to replace low molecular weight PEI and the neuron targeting peptide, we applied the principle of our previous study to generate self-assembled complexes in the current study.…”

Section: Discussionmentioning

confidence: 99%

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Gene transfer using self-assembled ternary complexes of cationic magnetic nanoparticles, plasmid DNA and cell-penetrating Tat peptide

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene transfer using self-assembled ternary complexes of cationic magnetic nanoparticles, plasmid DNA and cell-penetrating Tat peptide

et al. 2010

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“…Covalently linking the target molecules to liposomes is a prevalent method of conjugation, but this process may attenuate the activity of certain targeting molecules (Kocbek et al, 2007;Nobs et al, 2004;Zeng et al, 2007). Noncovalent combination is another convenient method, but this interaction is too weak to stabilize the molecules on the particles (Nobs et al, 2004;Sun et al, 2008).…”

Section: Utility Of Immunostaining For Flow Cytometrymentioning

confidence: 99%

“…This process may attenuate the activity of certain targeting molecules during the coupling reaction (Kocbek et al, 2007;Nobs et al, 2004;Zeng et al, 2007). One way to avoid this drawback involves the noncovalent adhesion of targeting molecules to a cationic liposome.…”

Section: Introductionmentioning

confidence: 98%

A unique and potent protein binding nature of liposome containing polyethylenimine and polyethylene Glycol: A nondisplaceable property

Liu

Lin

Chen

et al. 2011

Biotech & Bioengineering

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Most of the currently available targeting vectors are produced via the linkage of targeting molecules. However, the coupling process is complicated, and the covalent linkage may attenuate the activity of certain targeting molecules. In this study, we have developed a cationic liposome complexed with polyethylenimine and polyethylene glycol polymers (LPPC) that can capture various proteins without covalent conjugation. Characterizations of prepared LPPC revealed that the maximal-binding capacity was about 170 µg of bovine serum albumin to 40 µg of sphere-shaped LPPC (180 nm). The proteins were essentially located at or near the surface when analyzed by atomic force or transmission electron microscopy. We demonstrate that polyethylenimine was an essential component to bind the proteins. Upon the saturation of captured proteins, a given protein could not be displaced by other additional proteins and still retained its biological activity. Using a variety of functional proteins, we show some typical examples of the utility of incorporated beta-glucuronidase and antibodies onto the LPPC. The beta-glucuronidase can be used for the study of antigen-antibody interactions, whereas in studies with the antibody complex, we used anti-CD3 as an agonist to stimulate the proliferation of peripheral blood mononuclear cells via a receptor-mediated mechanism and anti-VEGFR for cell staining. In conclusion, the prepared LPPC can provide a platform to capture biologically and biochemically functional proteins on its surface for various applications, such as cell signaling, cell profiling, noncovalent enzyme-linked immunoassays, and others not mentioned.

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“…Chemical structures of chitosan and its derivatives; 1. PEG (Harris et al, 1984); 2. trimethylated (Zeng et al, 2007); 3. folic acid (Mansouri et al, 2006;Fernandes et al, 2008); 4.galactosylated (Park et al, 2001); 5. Arginine (Morris et al, 2010); 6. histidine; 7.…”

Section: General Strategies For Chitosan Modificationmentioning

confidence: 99%

Chitosan-DNA/siRNA Nanoparticles for Gene Therapy

Shi¹,

Tiera²,

Xiao-ling³

et al. 2011

Non-Viral Gene Therapy

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Self-assembled ternary complexes of plasmid DNA, low molecular weight polyethylenimine and targeting peptide for nonviral gene delivery into neurons

Cited by 54 publications

References 29 publications

Gene transfer using self-assembled ternary complexes of cationic magnetic nanoparticles, plasmid DNA and cell-penetrating Tat peptide

Gene transfer using self-assembled ternary complexes of cationic magnetic nanoparticles, plasmid DNA and cell-penetrating Tat peptide

A unique and potent protein binding nature of liposome containing polyethylenimine and polyethylene Glycol: A nondisplaceable property

Chitosan-DNA/siRNA Nanoparticles for Gene Therapy

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