Self-Assembled Proteins and Peptides for Regenerative Medicine

Hosseinkhani, Hossein; Hong, Po‐Da; Yü, Dah‐Shyong

doi:10.1021/cr300131h

Cited by 262 publications

(194 citation statements)

References 247 publications

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“…Specifically, nanoscale surface topography could promote cell-to-cell contact and an RAD motif of PuraMatrix, which is similar to the sequence of RGD identified as a cell attachment sequence in various adhesive proteins present in the ECM, thus providing support for cell adhesion, migration, and proliferation. 8,10,11 Furthermore, the findings using our model suggest that the remnant subepithelial layer of the recipient middle-ear seems to facilitate homing of donor cells to their destination.…”

Section: Discussionmentioning

confidence: 67%

“…[6][7][8][9] Tissue engineering requires scaffolds that serve as substrates for seeding cells, provide physical support that guides new tissue formation, promote the regeneration of natural tissues, or enhance the creation of biological substitutes for defective or lost organs. 10,11 Many types of scaffolds, such as biological extracellular matrix (ECM) and biological or synthetic hydrogels, have been developed for cell transplantation over the last several decades. Biological ECM, eg, Matrigel (BD Biosciences, San Jose, CA, USA), which consists of type IV collagen, laminin, and heparan sulfate proteoglycan, seems to help create a suitable microenvironment.…”

Section: Introductionmentioning

confidence: 99%

“…10,15 One of the attractive self-assembling peptide hydrogels used as three-dimensional scaffolds for tissue engineering comprises ionic self-complementary peptides, which form stable β-sheet structures that self-assemble to form nanofibers. 11 These nanofibers form interwoven matrices that further form a high-water-content scaffold hydrogel.…”

Section: Introductionmentioning

confidence: 99%

“…8 Moreover, these peptides have the motif arginine-alanineaspartic acid (RAD), which is similar to the ubiquitous integrin receptor-binding site arginine-glycine-aspartic acid (RGD), which has been studied in the context of cell attachment. 8,11,24 At present, several clinical trials of cell therapies using PuraMatrix have been reported for cartilage, 25,26 myocardial, 27 neural, [28][29][30][31] and hepatic 32,33 regeneration, indicating the potential usefulness of PuraMatrix.…”

Section: Introductionmentioning

confidence: 99%

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In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Akiyama¹,

Yamamoto‐Fukuda²,

Takahashi³

et al. 2013

IJN

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Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle-ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle-ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle-ear in SD rats.

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Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Akiyama¹,

Yamamoto‐Fukuda²,

Takahashi³

et al. 2013

IJN

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“…In a recent work, mucosal cells loaded in a self-assembling peptide scaffold Puramatrix® (RADA16) were successfully employed for middle ear tissue engineering [82]. A recent review of self-assembling proteins and peptides for regenerative medicine has discussed in detail the wide-ranging applications of self-assembling peptide scaffolds for cartilage, bone, nerve, cardiac and tooth tissue engineering [83][84][85][86][87].…”

Section: Self-assembled Peptide Nanofibres For Tissue Engineeringmentioning

confidence: 99%

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

Krishnan

Sethuraman

2013

Nanomaterials and Nanotechnology

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Introduction: Successful tissue engineering strategies leading to the regeneration of a tissue depend on many factors, starting from the choice of appropriate scaffold material, tailoring the surface functionalities and topography, providing the correct amount of chemical and mechanical stimuli at the appropriate time points, and ensuring the uniform and precise localization of cells. Further challenges arise when more than one cell type has to be employed for the effective regeneration of an organ. Importance: Though the use of nanomaterials has improved tissue engineering, many pitfalls still exist that present a roadblock in the translation of tissue engineering strategies to clinical practice. Apart from employing different materials with distinct surface functionalities and mechanical properties, various strategies have been employed to manipulate the surface topography and chemistry of scaffolds to create a biomimetic microenvironment for effective tissue regeneration. Conclusion: This review provides information about the factors influencing tissue engineering, namely geometry, chemistry, mechanics and cells, and the emerging concepts that may well represent the future of regenerative medicine. Electrospinning techniques and their variants, self-assembly, cell-printing techniques and cell sheet engineering, have all been elaborated in detail. These novel techniques may serve to overcome the challenges currently faced in tissue engineering.

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