Self-assembled proteinaceous wound dressings attenuate secondary trauma and improve wound healing <i>in vivo</i>

Zhao, Jian; Qu, Yangcui; Chen, Hong; Xu, Rui; Yu, Qian; Yang, Peng

doi:10.1039/c8tb01100a

Cited by 59 publications

(51 citation statements)

References 59 publications

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“…At present, most medical dressings still use traditional cotton gauze. Nevertheless, traditional cotton gauze needs to be replaced frequently, and easily adheres to the wound, which can easily lead to secondary tissue trauma and bacterial breeding [4,5,6]. Moreover, huge dressing wastes cause great harm to the environment.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

Chen

Guo

et al. 2019

Nanomaterials

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Traditional wound dressings require frequent replacement, are prone to bacterial growth and cause a lot of environmental pollution. Therefore, biodegradable and antibacterial dressings are eagerly desired. In this paper, gelatin/ZnO fibers were first prepared by side-by-side electrospinning for potential wound dressing materials. The morphology, composition, cytotoxicity and antibacterial activity were characterized by using Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), particle size analyzer (DLS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), thermogravimetry (TGA) and Incucyte™ Zoom system. The results show that ZnO particles are uniformly dispersed on the surface of gelatin fibers and have no cytotoxicity. In addition, the gelatin/ZnO fibers exhibit excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with a significant reduction of bacteria to more than 90%. Therefore, such a biodegradable, nontoxic and antibacterial fiber has excellent application prospects in wound dressing.

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Section: Introductionmentioning

confidence: 99%

Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

Chen

Guo

et al. 2019

Nanomaterials

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“…In this process, the disulfide bonds of native lysozyme were reduced efficiently by TCEP, [ 24 ] and then a PTL/C‐AMG nanofilm was formed on the silicon wafer surface, which was actually composed of densely packed oligomeric nanoparticles with an average diameter of 50 nm, as revealed by AFM and transmission electron microscopy (TEM; Figure 1b,c and Figure S4, Supporting Information), similar to the oligomeric nanoparticles structures of the pure PTL film reported previously. [ 24,26,29,30 ] The PTL/C‐AMG film thickness could be regulated from 3 to 42 nm by changing the incubation time from 1 to 50 min (Figure S5, Supporting Information), and such film on the silicon surface did not show obvious changes in surface topography after being subjected to various harsh conditions, such as extreme pH (2, 11), salt solution, high temperature, organic solvents, and adhesive tape peeling (Figure S6, Supporting Information). Similar to that of amelogenin‐forming amyloid structures, [ 10,11 ] the amyloid‐like structure of the PTL/C‐AMG nanofilm was identified by circular dichroism (CD) and Fourier transform infrared (FTIR) spectra, which showed an increase in the characteristic β‐sheet structure (218 nm in CD and 1625 cm −1 in FTIR) (Figure 1d,e).…”

Section: Methodsmentioning

confidence: 99%

Controlling Enamel Remineralization by Amyloid‐Like Amelogenin Mimics

et al. 2020

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In situ regeneration of the enamel‐like structure of hydroxyapatite (HAp) crystals under oral conditions is significant for dental caries treatment. However, it is still a challenge for dentists to duplicate the elegant and well‐aligned apatite structure bonding to the surface of demineralized enamel. A biocompatible amelogenin‐inspired matrix, a phase‐transited lysozyme (PTL) film mimicking an N‐terminal amelogenin with central domain (N‐Ame) combined with synthetic peptide (C‐AMG) based on the functional domains of C‐terminal telopeptide (C‐Ame) is shown here, which is formed by amyloid‐like lysozyme aggregation at the enamel interface through a rapid one‐step aqueous coating process. In the PTL/C‐AMG matrix, C‐AMG facilitated the oriented arrangement of amorphous calcium phosphate (ACP) nanoparticles and their transformation to ordered enamel‐like HAp crystals, while PTL served as a strong interfacial anchor to immobilize the C‐AMG peptide and PTL/C‐AMG matrix on versatile substrate surfaces. PTL/C‐AMG film‐coated enamel induced both of the in vivo and in vitro synthesis of HAp crystals, facilitated epitaxial growth of HAp crystals and recovered the highly oriented structure and mechanical properties to levels nearly identical to those of natural enamel. This work underlines the importance of amyloid‐like protein aggregates in the biomineralization of enamel, providing a promising strategy for treating dental caries.

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“…Zhao et al found the PTLF could be degraded and detached from the substrate surface in the presence of vitamin C. [ 119 ] Later, Li et al designed an on‐demand degradable boron carrier based on this feature of PTLF to treat triple‐negative breast cancer (Figure 10b). [ 120 ] Boron nitride nanoparticles (BNNPs) were coated by a PTLF, which could protect them from hydrolysis to prolong blood circulation.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Amyloid‐Mediated Fabrication of Organic–Inorganic Hybrid Materials and Their Biomedical Applications

Zhao

Yang

2020

Adv Materials Inter

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nanostructures can be organized into a hierarchical architecture, thus giving their excellent physical and chemical properties. [1,6,7] Accordingly, protein-based biomimetic mineralization has been became an inspiration for synthesis of the highperformance organic-inorganic hybrid materials. Recently, many reviews have been published to report the progress of biomimetic organic-inorganic nanocomposites. Wang et al. reviewed the preparation of layered organic-inorganic nanocomposites inspired by nacre and indicated the advantages and disadvantages of various biomimetic strategies. [8] Zhang et al. summarized recent research on graphene-based artificial nacre nanocomposites and focused on the interface interactions design. [9] Wang et al. discussed functional protein-based organic-inorganic hybrid nanomaterials with an emphasis on the novel preparation methods, resulting nanostructures, and their potential applications in drug delivery and enzymatic catalysis. [10] Yao et al. summarized the fabrication of artificial layered structural nanocomposites based on the different inorganic building blocks, such as clays, ceramic nanoparticles, layered double hydroxides, calcium carbonate, and graphene. [11] Zhao et al. overviewed the synthesis and performance enhancement strategies for different dimensional nacre-inspired materials such as 1D fibers, 2D films, and 3D bulk composites. [6] The reviews mentioned above are mainly focused on the strategies to construct layered organic-inorganic hybrid materials and their applications in various fields, and the interface design to obtain high-performance structural nanocomposites. Although it has been demonstrated that the protein components function as templates involving in the biomineralization, the regulation on their structure and the mediation of proteins on the fabrication of hybrid materials are seldom presented, mainly because of their complex molecular structure and uncontrollable selfassembly process in vitro. Amyloid is a kind of insoluble self-assembly peptide or protein aggregate displaying polymorphic mesoscopic structures. [12] Even though the amyloids are originally discovered as the pathological hallmarks of human neurodegenerative diseases, it is found that there are also many nonpathogenic functional amyloid aggregates implicated in biological processes occurring in a variety of organisms ranging from simple bacteria to humans, such as the adhesive property of E. coil biofilms, [13] biosynthesis Protein assembly with ordered architecture plays an important role in the formation of natural organic-inorganic hybrid materials, and also determines their distinguished mechanical, optical, and biochemical properties. Nevertheless, it has been a huge challenge to synthesize materials with hierarchical structure accurately mimicking the natural hybrid materials in vitro. In the past decade, protein amyloid aggregates, due to their diverse and controllable nanostructures and unique properties, have been developed as superior templates or building blocks to fabricate f...

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Self-assembled proteinaceous wound dressings attenuate secondary trauma and improve wound healing in vivo

Abstract: Stimuli-responsive gauze coated with a phase-transitioned lysozyme nanofilm (PTLF@gauze) has been developed, which exhibits great potential for clinical applications by reducing secondary trauma and relieving the pain of patients.

Cited by 59 publications

References 59 publications

Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

Controlling Enamel Remineralization by Amyloid‐Like Amelogenin Mimics

Amyloid‐Mediated Fabrication of Organic–Inorganic Hybrid Materials and Their Biomedical Applications

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