Self-assembled polysaccharide nanostructures for controlled-release applications

Myrick, James M.; Vendra, Venkat Kalyan; Krishnan, Sitaraman

doi:10.1515/ntrev-2012-0050

Cited by 76 publications

(40 citation statements)

References 125 publications

(154 reference statements)

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“…It is well known that acidic polysaccharides are prone to forming spheroidal architectures in aqueous solution by means of calcium-mediated gelation. A typical instance is the Ca 2þ -alginate nanoparticles formed via self-assembly in aqueous environments [42][43][44][45]. In light of this, given that divalent cations, i.e.…”

Section: Nanoparticles Observed In Sundew Adhesivementioning

confidence: 99%

Sundew adhesive: a naturally occurring hydrogel

Huang

Wang

Sun

et al. 2015

J. R. Soc. Interface.

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Bioadhesives have drawn increasing interest in recent years, owing to their ecofriendly, biocompatible and biodegradable nature. As a typical bioadhesive, sticky exudate observed on the stalked glands of sundew plants aids in the capture of insects and this viscoelastic adhesive has triggered extensive interests in revealing the implied adhesion mechanisms. Despite the significant progress that has been made, the structural traits of the sundew adhesive, especially the morphological characteristics in nanoscale, which may give rise to the viscous and elastic properties of this mucilage, remain unclear. Here, we show that the sundew adhesive is a naturally occurring hydrogel, consisting of nanonetwork architectures assembled with polysaccharides. The assembly process of the polysaccharides in this hydrogel is proposed to be driven by electrostatic interactions mediated with divalent cations. Negatively charged nanoparticles, with an average diameter of 231.9 + 14.8 nm, are also obtained from this hydrogel and these nanoparticles are presumed to exert vital roles in the assembly of the nano-networks. Further characterization via atomic force microscopy indicates that the stretching deformation of the sundew adhesive is associated with the flexibility of its fibrous architectures. It is also observed that the adhesion strength of the sundew adhesive is susceptible to low temperatures. Both elasticity and adhesion strength of the sundew adhesive reduce in response to lowering the ambient temperature. The feasibility of applying sundew adhesive for tissue engineering is subsequently explored in this study. Results show that the fibrous scaffolds obtained from sundew adhesive are capable of increasing the adhesion of multiple types of cells, including fibroblast cells and smooth muscle cells, a property that results from the enhanced adsorption of serum proteins. In addition, in light of the weak cytotoxic activity exhibited by these scaffolds towards a variety of mammal cells, evidence is sufficient to propose that sundew adhesive is a promising nanomaterial worth further exploitation in the field of tissue engineering.

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Section: Nanoparticles Observed In Sundew Adhesivementioning

confidence: 99%

Sundew adhesive: a naturally occurring hydrogel

Huang

Wang

Sun

et al. 2015

J. R. Soc. Interface.

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“…Signal‐triggered release of substances from chemical systems for various applications , particularly drug release , and more specifically DNA release , for biomedical applications has been extensively studied in the last decades. The controlled DNA release is important for many applications, including gene‐delivery therapy , biosensors , biochips and biocomputing .…”

Section: Introductionmentioning

confidence: 99%

Electrochemically Triggered DNA Release from a Mixed‐brush Polymer‐modified Electrode

et al. 2016

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1IntroductionSignal-triggered release of substances fromc hemicals ystems for variousa pplications [1][2][3][4],p articularly drug release [5][6][7][8][9][10][11][12],a nd more specifically DNAr elease [13,14], for biomedical applications has been extensively studied in the last decades.T he controlled DNAr elease is important for many applications,i ncludingg ene-delivery therapy [13,15],b iosensors [16],b iochips [17] and biocomputing [18].V ariousm ethodso ft he signal-controlled DNA release have been reported recently,i ncluding irradiation of DNA-loaded nanoparticles with an alternating electromagnetic field [19],l ight-triggered capture and release of DNA[ 20],t hermal release of DNAf rom nanostructured systems [ 21,22],e tc. Among different methodso ft he DNAc ontrolled release,e lectrochemically stimulated release of DNAp re-loaded on an electrode surface is particularly convenient and interesting due to its simplicity and versatility of the method. Most of the systems developed for the electrochemically stimulated DNAr elease are based on disassembly of polymeric matrices loaded with DNAm olecules.T his was achieved by decomposition of electrostatically bound multi-layered polymeric structures loaded with DNA [23][24][25][26] or by splittingc ovalent bonds between DNAm olecules and am odified electrode surface [27].T he disadvantage of these approaches is irreversible degradation of the assemblies on the electrode surfaces,w hich allowso nly one-time release process.A nother approach, which allows multiple use of the functional interface,c apable of reversible loading-unloading of DNAm oleculeso nt he electrode surface,i sb ased on electrostatic attraction/repulsion processes for the charged DNAm olecules upon variation of the electrode potential and corresponding charge [28][29][30][31][32].T he reversi-ble adsorption and desorption processes of the negatively charged DNAm olecules are controlled by the charge imposed on the electrode surface upon application of variable potentials [33].W hile the experimental realization of the potential-controlled DNAa dsorption/desorption process has been studied recently,t he theoretical background for this phenomenon wase stablished in the research of A. N. Frumkin early in 1920s [34].M odernn anotechnological advances allowed DNAr elease inside living cells fromn ano-size electrode needles inserted into the cells [35].While electrostatically controlled DNAa dsorption on an electrode surface followed by its desorption upon the change of the applied potential is av ery simple and powerful method,a lso allowing multiple reversible deposition and release of DNA, it hasa ni nherent drawback. Indeed, az ero-charge potential depends on the electrode environment and adsorption of other biomolecules, particularly,w hen the electrode operates in biofluidsw ith complex biomolecularc omposition [36].T his can shift the potential regionsw here the electrode is positively charged for the electrostatic DNAd eposition and negatively charged for the DNAr epulsion and release.T her...

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“…The height analysis in the planar image ( Figure 5E,F) revealed the aggregation extent of SAZMP4. A branched, ring-like, helical or an interconnected network structure in the AFM image ( Figure 5A,B) implied molecular self-assembly of SAZMP4, which undergoes a spontaneous process from disorder to order based on the weak, noncovalent interaction of hydrogen bonds and van der Waals forces as well as the hydrophobic effect [41]. These results indicated that SAZMP4 might aggregate at first and then self-assemble to form a long chain; this is why the molecular morphology of SAZMP4 appeared the way it did in SEM and AFM images.…”

Section: Molecular Morphological Propertiesmentioning

confidence: 99%

A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

Lin

Liu

et al. 2020

Antioxidants

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After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H 2 O 2 -induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive.

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Self-assembled polysaccharide nanostructures for controlled-release applications

Cited by 76 publications

References 125 publications

Sundew adhesive: a naturally occurring hydrogel

Sundew adhesive: a naturally occurring hydrogel

Electrochemically Triggered DNA Release from a Mixed‐brush Polymer‐modified Electrode

A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

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