Self Assembled Ionically Sodium Alginate Cross-Linked Amphotericin B Encapsulated Glycol Chitosan Stearate Nanoparticles: Applicability in Better Chemotherapy and Non-Toxic Delivery in Visceral Leishmaniasis

Gupta, Pramod; Jaiswal, Anil K.; Asthana, Shalini; Verma, Ayushi; Kumar, Vivek; Shukla, P.K.; Dwivedi, Pankaj; Dube, Anuradha; Mishra, Prabhat Ranjan

doi:10.1007/s11095-014-1571-4

Cited by 54 publications

(43 citation statements)

References 42 publications

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“…The results of in vitro tests (promastigote and amastigote) showed that AK nanodrug was not very effective in parasite killing. However, they demonstrated an only negligible effect on inhibiting parasite in vivo environment, which is possibly due to a low dose of AK1 mg/kg (7- 10,12,13,15,16,24). In the present study, A was loaded into K synthesized by phase separation method (different from previous studies by using ionic gelation) and it was found that K decreased the toxicity effects of A and increased its solubility (14,18).…”

Section: Discussionmentioning

confidence: 55%

In Vivo Therapeutic Effects of Four Synthesized Antileishmanial Nanodrugs in the Treatment of Leishmaniasis

Mehrizi

Mosaffa

Hoseini

et al. 2018

Arch Clin Infect Dis

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Background: Nowadays, nanocarriers are used for leishmaniasis treatment due to development of drug resistance and several side effects with conventional therapeutics. Objectives: In this study we aimed to evaluate in vivo effects of four synthesized nanodrugs including amphotericin B-nanochitosan (AK), betulinic acid-nanochitosan (BK), amphotericin B-dendrimer (AD), and betulinic acid-dendrimer (BD) in the treatment of Leishmania major infection (L. major) in mice model by using pathological analyses to choose the most effective nanodrug in leishmaniasis. Methods: The four nanodrugs efficacy in the improvement of L. major lesion in a mice model was evaluated by using pathological analyses including measurement of organs size and parasite number. Additionally, the nanodrugs toxicity was evaluated by measurement of various blood factors. Results: The histopathological results of the present study showed that BK, at the dose of 20 mg/kg, and AK, at the dose of 10 mg/kg, were more effective in decreasing the parasite number in the kidney, liver, and spleen. Moreover, BK20 mg/kg and AK10 mg/kg decreased the organs size significantly while AD50 mg/kg and BD40 mg/kg were less effective. However, none of the four nanodrugs had increased the blood factors and they were not toxic. Conclusions: Overall, the pathologic findings of various mice organs treated with different formulations showed that AK10 mg/kg and BK20 mg/kg were more effective in recovery of L. major's pathological effects in comparison to AD50 mg/kg and BD40 mg/kg. Therefore, it seems that AK and BK, in this mentioned dosage, could be considered as a proper candidate for treatment of leishmaniasis.

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Section: Discussionmentioning

confidence: 55%

In Vivo Therapeutic Effects of Four Synthesized Antileishmanial Nanodrugs in the Treatment of Leishmaniasis

Mehrizi

Mosaffa

Hoseini

et al. 2018

Arch Clin Infect Dis

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“…Mannosylated nanocarriers have been shown to generate signals following engulfment or interaction with phagocytic cells, in particular macrophages, which leads to macrophage stimulation and upregulation of cytokines [17]. Inducible nitric oxide synthase and consecutive generation of nitric oxide has been shown to exhibit antimicrobial effect [27]. The chitosan polymer has been reported to trigger a proinflammatory response such as nitric oxide production that stimulates macrophages and this could lead to parasiticidal activity [17,27].…”

Section: Discussionmentioning

confidence: 99%

“…Inducible nitric oxide synthase and consecutive generation of nitric oxide has been shown to exhibit antimicrobial effect [27]. The chitosan polymer has been reported to trigger a proinflammatory response such as nitric oxide production that stimulates macrophages and this could lead to parasiticidal activity [17,27]. Hence, antileishmanial activity of drug-free nanocarriers based on chitosan polymeric backbone could be due to nitric oxide production.…”

Section: Discussionmentioning

confidence: 99%

Development of mannose-anchored Thiolated Amphotericin B Nanocarriers for Treatment of Visceral Leishmaniasis

Shahnaz

Edagwa

McMillan

et al. 2016

Nanomedicine (Lond.)

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Aim:Our goal was to improve treatment outcomes for visceral leishmaniasis by designing nanocarriers that improve drug biodistribution and half-life. Thus, long-acting mannose-anchored thiolated chitosan amphotericin B nanocarrier complexes (MTC AmB) were developed and characterized.Materials & methods:A mannose-anchored thiolated chitosan nanocarrier was manufactured and characterized. MTC AmB was examined for cytotoxicity, biocompatibility, uptake and antimicrobial activities.Results:MTC AmB was rod shaped with a size of 362 nm. MTC AmB elicited 90% macrophage viability and 71-fold enhancement in drug uptake compared with native drug. The antileishmanial IC50 for MTC AmB was 0.02 μg/ml compared with 0.26 μg/ml for native drug.Conclusion:These studies show that MTC can serve as a platform for clearance of Leishmania in macrophages.

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“…A more complex system, consisting of self assembled sodium alginate cross-linked AmB loaded glycol chitosan stearate nanoparticles (AmB-SA-GCS-NP) was prepared using strong electrostatic interactions between oppositely charged polymer and copolymer using the ionotropic complexation method [ 37 ]. The nanocrystals obtained exhibited a size of 196 nm.…”

Section: Chitosan-based Drug Loaded Formulations For the Chemothermentioning

confidence: 99%

Chitosan Contribution to Therapeutic and Vaccinal Approaches for the Control of Leishmaniasis

2020

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The control of leishmaniases, a complex parasitic disease caused by the protozoan parasite Leishmania, requires continuous innovation at the therapeutic and vaccination levels. Chitosan is a biocompatible polymer administrable via different routes and possessing numerous qualities to be used in the antileishmanial strategies. This review presents recent progress in chitosan research for antileishmanial applications. First data on the mechanism of action of chitosan revealed an optimal in vitro intrinsic activity at acidic pH, high-molecular-weight chitosan being the most efficient form, with an uptake by pinocytosis and an accumulation in the parasitophorous vacuole of Leishmania-infected macrophages. In addition, the immunomodulatory effect of chitosan is an added value both for the treatment of leishmaniasis and the development of innovative vaccines. The advances in chitosan chemistry allows pharmacomodulation on amine groups opening various opportunities for new polymers of different size, and physico-chemical properties adapted to the chosen routes of administration. Different formulations have been studied in experimental leishmaniasis models to cure visceral and cutaneous leishmaniasis, and chitosan can act as a booster through drug combinations with classical drugs, such as amphotericin B. The various architectural possibilities given by chitosan chemistry and pharmaceutical technology pave the way for promising further developments.

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Self Assembled Ionically Sodium Alginate Cross-Linked Amphotericin B Encapsulated Glycol Chitosan Stearate Nanoparticles: Applicability in Better Chemotherapy and Non-Toxic Delivery in Visceral Leishmaniasis

Abstract: The molecular organization, toxicity studies, desired localization and biodistribution of cost effective AmB-SA-GCS-NP was found to be highly effective and can be proved as practical delivery platform for better management of leishmaniasis.

Cited by 54 publications

References 42 publications

In Vivo Therapeutic Effects of Four Synthesized Antileishmanial Nanodrugs in the Treatment of Leishmaniasis

In Vivo Therapeutic Effects of Four Synthesized Antileishmanial Nanodrugs in the Treatment of Leishmaniasis

Development of mannose-anchored Thiolated Amphotericin B Nanocarriers for Treatment of Visceral Leishmaniasis

Chitosan Contribution to Therapeutic and Vaccinal Approaches for the Control of Leishmaniasis

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