Self-assembled innervated vasculature-on-a-chip to study nociception

Kumar, Vardhman; Kingsley, David M.; Perikamana, Sajeeshkumar Madhurakkat; Mogha, Pankaj; Goodwin, C. Rory; Varghese, Shyni

doi:10.1088/1758-5090/acc904

Cited by 6 publications

(7 citation statements)

References 71 publications

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“…Other models combined endothelial cells and neurons to examine neurovascular physiology and disease. One such study built a model of nociception using primary dorsal root ganglion neurons from mice or humans [24 ▪ ]. Notably, the neurons were more sensitive to capsaicin in the vascular MPS model compared to the model lacking vasculature.…”

Section: Organ Specificitymentioning

confidence: 99%

Vascular microphysiological systems

Shelton

2024

Current Opinion in Hematology

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Purpose of review This review summarizes innovations in vascular microphysiological systems (MPS) and discusses the themes that have emerged from recent works. Recent findings Vascular MPS are increasing in complexity and ability to replicate tissue. Many labs use vascular MPS to study transport phenomena such as analyzing endothelial barrier function. Beyond vascular permeability, these models are also being used for pharmacological studies, including drug distribution and toxicity modeling. In part, these studies are made possible due to exciting advances in organ-specific models. Inflammatory processes have also been modeled by incorporating immune cells, with the ability to explore both cell migration and function. Finally, as methods for generating vascular MPS flourish, many researchers have turned their attention to incorporating flow to more closely recapitulate in vivo conditions. Summary These models represent many different types of tissue and disease states. Some devices have relatively simple geometry and few cell types, while others use complex, multicompartmental microfluidics and integrate several cell types and origins. These 3D models enable us to observe model evolution in real time and perform a plethora of functional assays not possible using traditional cell culture methods.

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Section: Organ Specificitymentioning

confidence: 99%

Vascular microphysiological systems

Shelton

2024

Current Opinion in Hematology

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“…Sensory neurons extensively populate peripheral tissues, detecting and reacting to harmful mechanical, thermal, and chemical stimuli. Nociceptor afferents are equipped with ligand-and voltage-gated ion channels, serving as essential molecular transducers for noxious stimuli [29]. Another chemically harmful stimulus of clinical significance is linked to tissue acidosis, a state marked by an elevated concentration of protons, indicated by a low pH [30].…”

Section: Utilizing a Self-assembled Innervated Vasculature-on-a-chip ...mentioning

confidence: 99%

Tiny Organs, Big Impact: How Microfluidic Organ-on-Chip Technology Is Revolutionizing Mucosal Tissues and Vasculature

Dasgupta,

Rangineni,

Abdelsaid

et al. 2024

Bioengineering

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Organ-on-chip (OOC) technology has gained importance for biomedical studies and drug development. This technology involves microfluidic devices that mimic the structure and function of specific human organs or tissues. OOCs are a promising alternative to traditional cell-based models and animals, as they provide a more representative experimental model of human physiology. By creating a microenvironment that closely resembles in vivo conditions, OOC platforms enable the study of intricate interactions between different cells as well as a better understanding of the underlying mechanisms pertaining to diseases. OOCs can be integrated with other technologies, such as sensors and imaging systems to monitor real-time responses and gather extensive data on tissue behavior. Despite these advances, OOCs for many organs are in their initial stages of development, with several challenges yet to be overcome. These include improving the complexity and maturity of these cellular models, enhancing their reproducibility, standardization, and scaling them up for high-throughput uses. Nonetheless, OOCs hold great promise in advancing biomedical research, drug discovery, and personalized medicine, benefiting human health and well-being. Here, we review several recent OOCs that attempt to overcome some of these challenges. These OOCs with unique applications can be engineered to model organ systems such as the stomach, cornea, blood vessels, and mouth, allowing for analyses and investigations under more realistic conditions. With this, these models can lead to the discovery of potential therapeutic interventions. In this review, we express the significance of the relationship between mucosal tissues and vasculature in organ-on-chip (OOC) systems. This interconnection mirrors the intricate physiological interactions observed in the human body, making it crucial for achieving accurate and meaningful representations of biological processes within OOC models. Vasculature delivers essential nutrients and oxygen to mucosal tissues, ensuring their proper function and survival. This exchange is critical for maintaining the health and integrity of mucosal barriers. This review will discuss the OOCs used to represent the mucosal architecture and vasculature, and it can encourage us to think of ways in which the integration of both can better mimic the complexities of biological systems and gain deeper insights into various physiological and pathological processes. This will help to facilitate the development of more accurate predictive models, which are invaluable for advancing our understanding of disease mechanisms and developing novel therapeutic interventions.

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“…This nerve-on-a-chip demonstrated suitability in an in vitro model for capturing and enhancing the electrical activity of dorsal root ganglion (DRG) cells [40]. Primary dorsal root ganglion (DRG) cells are used to model nerve-on-a-chip for preclinical toxic effect drug testing, since nervous cells are particularly vulnerable to adverse effects of chemotherapies [39,41]. Kumar et al also fabricated a self-assembly innervated microvasculature-on-a-chip by co-culturing endothelial and DRG cells to study the impact of nervous tissue on the vascular system [39].…”

Section: Tooth-on-a-chip Designs and Materialsmentioning

confidence: 99%

“…Primary dorsal root ganglion (DRG) cells are used to model nerve-on-a-chip for preclinical toxic effect drug testing, since nervous cells are particularly vulnerable to adverse effects of chemotherapies [39,41]. Kumar et al also fabricated a self-assembly innervated microvasculature-on-a-chip by co-culturing endothelial and DRG cells to study the impact of nervous tissue on the vascular system [39]. The tooth has a peculiar anatomy in which innervation is part of the pulp, as well as vasculature, and plays a key role during the phases of teeth development.…”

Section: Tooth-on-a-chip Designs and Materialsmentioning

confidence: 99%

A Systematic Review on Organ-on-a-Chip in PDMS or Hydrogel in Dentistry: An Update of the Literature

Pierfelice,

D’Amico,

Petrini

et al. 2024

Gels

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Organs-on-a-chip (OoCs) are microfluidic devices constituted by PDMS or hydrogel in which different layers of cells are separated by a semipermeable membrane. This technology can set many parameters, like fluid shear stress, chemical concentration gradient, tissue–organ interface, and cell interaction. The use of these devices in medical research permits the investigation of cell patterning, tissue–material interface, and organ–organ interaction, mimicking the complex structures and microenvironment of human and animal bodies. This technology allows us to reconstitute in vitro complex conditions that recapitulate in vivo environments. One of the main advantages of these systems is that they represent a very realistic model that, in many cases, can replace animal experimentation, eliminating costs and related ethical issues. Organ-on-a-chip can also contain bacteria or cancer cells. This technology could be beneficial in dentistry for testing novel antibacterial substances and biomaterials, performing studies on inflammatory disease, or planning preclinical studies. A significant number of publications and reviews have been published on this topic. Still, to our knowledge, they mainly focus on the materials used for fabrication and the different patterns of the chip applied to the experimentations. This review presents the most recent applications of organ-on-a-chip models in dentistry, starting from the reconstituted dental tissues to their clinical applications and future perspectives.

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Self-assembled innervated vasculature-on-a-chip to study nociception

Cited by 6 publications

References 71 publications

Vascular microphysiological systems

Vascular microphysiological systems

Tiny Organs, Big Impact: How Microfluidic Organ-on-Chip Technology Is Revolutionizing Mucosal Tissues and Vasculature

A Systematic Review on Organ-on-a-Chip in PDMS or Hydrogel in Dentistry: An Update of the Literature

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