Self-assembled gold nanoparticles for impedimetric and amperometric detection of a prostate cancer biomarker

Jolly, Pawan; Zhurauski, Pavel; Hammond, Jules L.; Miodek, Anna; Liébana, Susana; Bertók, Tomáš; Tkáč, Ján; Estrela, Pedro

doi:10.1016/j.snb.2017.05.040

Cited by 55 publications

(22 citation statements)

References 36 publications

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“…Figure a shows that all biosensors have two linear ranges and thus show two calibration graphs. The colored region (pink) in the graph indicating the critical PSA level that the PSA should be according to the literature . The detection limits of the biosensors were calculated as 0.001 ng mL −1 for all and it can be seen that this region can be measured within the range.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, studies have shown that the number of painless and faster diagnosis is increasing based on detection of the PSA level . According to the reports the values are consider abnormal level of PSA higher than 4 ng mL −1 . However, in recent researches, scientists have conducted studies that the level of PSA is lower than 4 ng mL −1 (2.5 or 3 ng mL −1 ) may also refer to prostate cancer .…”

Section: Introductionmentioning

confidence: 99%

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High Sensitive Detection of Prostate Specific Antigen by Using Ferrocene Cored Asymmetric PAMAM Dendrimer Interface Screen Printed Electrodes

Çevik

2018

Electroanalysis

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In this study, electrochemical immunosensors were developed for the detection of prostate specific antigen (PSA) using ferrocene (Fc) and polyamidoamine dendrimer (PAMAM) constructs. The biosensor fabrication was designed by modifying the screen‐printed gold electrode (Au) with ferrocene cored dendrimers (FcPAMAM) synthesized in three different generations. The self‐assembled monolayer principle was followed, to obtain sensitive, selective and disposable electrodes. Therefore, the Au electrodes were modified with cysteamine (Cys) to obtain a functional surface for FcPAMAM dendrimers to bind. Dendrimer generations were attached to this surface using a cross‐linker (glutaraldehyde) so that a suitable surface was obtained for binding of biological components. The Monoclonal PSA antibody (anti‐PSA) was immobilized on the Au electrode surface which coated with dendrimer, and (Au/Cys/FcPAMAM/anti‐PSA) biosensing electrode was obtained. The PSA detection performances of electrochemical impedance spectroscopy (EIS) and Amperometry based immunosensors exhibited very low detection limits; 0.001 ng mL−1 and 0.1 pg mL−1, respectively. In addition, EIS and Amperometry based biosensors using Au/Cys/FcPAMAM/anti‐PSA sensing electrode were represented excellent linear ranges of 0.01 ng mL−1 to 100 ng mL−1 and 0.001 ng mL−1 to 100 ng mL−1. In order to determine the applicability recovery and selectivity tests were performed using three different proteins in human serum.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High Sensitive Detection of Prostate Specific Antigen by Using Ferrocene Cored Asymmetric PAMAM Dendrimer Interface Screen Printed Electrodes

Çevik

2018

Electroanalysis

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“…Gold nanoparticles-based aptasensors give a rapid and simple way of detecting PSA. With gold being relatively easy to work with, due to extensive previous research into gold nanoparticles, on the nanoscale and its ability to form complexes with proteins which can be cleaved by PSA, gold nanoparticles are fundamental to the assembly of sensors tailor-made for the PSA detection [32].…”

Section: Gold Np-based Aptasensorsmentioning

confidence: 99%

“…The majority of biosensors utilize a signal-on method, as does this example, where gold nanoparticles are attached onto a gold electrode for impedimetric detection of PSA [32]. This example of a sensor showcases the effect of adding gold nanoparticles to a sensor system on sensitivity.…”

Section: Gold Np-based Aptasensorsmentioning

confidence: 99%

Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Perry

Cortezon‐Tamarit

Pascu

2019

Front. Chem. Sci. Eng.

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Prostate cancer has a high incidence in men and remains the second cause of mortality due to cancer worldwide. As the development of the disease is greatly correlated to age, the identification of novel detection methods reliable, efficient, and cost effective is a matter of significant importance in the ageing population of western societies. The detection of the prostate specific antigen (PSA) in blood samples has been the preferred method for the detection and monitoring of prostate cancer over the past decades. Despite the indications against its use in massive population screening, PSA still remains the best studied biomarker for prostate cancer and the detection of its different forms and incorporation in multiplexed designs with other biomarkers still remains a highly valuable indicator in the theranostics of prostate cancer. The latest developments in the use of nanomaterials towards the construction of PSA biosensors are reviewed hereby. The incorporation of gold nanoparticles, silica nanoparticles and graphene nanostructures to biosensing devices has represented a big leap forward in terms of sensitivity, stability and miniaturization. Both electrochemical and optical detection methods for the detection of PSA will be reviewed herein.

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“…Gold has been broadly utilized in nanomedicine for the diagnosing purpose due to the outstanding merits, such as easy production method and fabrication process, chemically stable, and holds a great catalytic activity which can heading towards the formation of better biosensor platforms [20][21][22] . In the biosensing application, gold is a promising material and plays a tremendously role for the better and high-performance sensing by acting as electroactive intermediates 23 . This is because of the better conductivity and capable of providing a biocompatible surface for the immobilization of biomolecules in a better arrangement 24 .…”

mentioning

confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

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Field of generating a surface thin film is emerging broadly in sensing applications to obtain the quick and fast results by forming the high-performance sensors. Incorporation of thin film technologies in sensor development for the better sensing could be a promising way to attain the current requirements. This work predominantly delineates the fabrication of the dielectric sensor using two different sensing materials (Gold and Aluminium). conventional photolithography was carried out using silicon as a base material and the photo mask of the dielectric sensor was designed by AutocAD software. the physical characterization of the fabricated sensor was done by Scanning electron Microscope, Atomic Force Microscope, High Power Microscope and 3D-nano profiler. The electrical characterization was performed using Keithley 6487 picoammeter with a linear sweep voltage of 0 to 2 V at 0.01 V step voltage. By pH scouting, i-V measurements on the bare sensor were carried out, whereby the gold electrodes conducts a least current than aluminium dielectrodes. comparative analysis with pH scouting reveals that gold electrode is suitable under varied ionic strengths and background electrolytes, whereas aluminium electrodes were affected by the extreme acid (pH 1) and alkali (pH 12) solutions.

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Self-assembled gold nanoparticles for impedimetric and amperometric detection of a prostate cancer biomarker

Cited by 55 publications

References 36 publications

High Sensitive Detection of Prostate Specific Antigen by Using Ferrocene Cored Asymmetric PAMAM Dendrimer Interface Screen Printed Electrodes

High Sensitive Detection of Prostate Specific Antigen by Using Ferrocene Cored Asymmetric PAMAM Dendrimer Interface Screen Printed Electrodes

Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

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