Self‐Assembled Fluorodendrimers Combine the Features of Lipid and Polymeric Vectors in Gene Delivery

Wang, Hui; Wang, Yitong; Wang, Yu; Hu, Jingjing; Li, Tianfu; Li, Hongmei; Zhang, Qiang; Cheng, Yiyun

doi:10.1002/ange.201501461

Cited by 24 publications

(12 citation statements)

References 44 publications

(78 reference statements)

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“…The highly desirable features of an ideal gene carrier are efficiency in cellular uptake and high permeability in targeted tissues 46 . 3D tumor spheroids have been used as an ideal format for better understanding of the influence of the cellular microenvironment in tumor biology 47 - 49 .…”

Section: Resultsmentioning

confidence: 99%

Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy

Li¹,

Li²,

Wu³

et al. 2017

Theranostics

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Cancer stem cell-like cells (CSCL) are responsible for tumor recurrence associated with conventional therapy (e.g. surgery, radiation, and chemotherapy). Here, we developed a novel multifunctional nucleus-targeting nanoparticle-based gene delivery system which is capable of targeting and eradicating CSCL. These nanoparticles can facilitate efficient endosomal escape and spontaneously penetrate into nucleus without additional nuclear localization signal. They also induced extremely high gene transfection efficiency (>95%) even in culture medium containing 30% serum, which significantly surpassed that of some commercial transfection reagents, such as Lipofectamine 2000 and Lipofectamine 3000 etc. Especially, when loaded with the TRAIL gene, this system mediated remarkable depletion of CSCL. Upon systemic administration, the nanoparticles accumulated in tumor sites while sparing the non-cancer tissues and significantly inhibited the growth of tumors with no evident systemic toxicity. Taken together, our results suggest that these novel multifunctional, nucleus-targeting nanoparticles are a very promising in vivo gene delivery system capable of targeting CSCL and represent a new treatment candidate for improving the survival of cancer patients.

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Section: Resultsmentioning

confidence: 99%

Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy

Li¹,

Li²,

Wu³

et al. 2017

Theranostics

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“…Wang et al created a fluorinated dendrimer in order to improve gene transfection efficiency. They tested the fabricated complex on various cancer cell lines and discovered that the fluorinated dendrimer achieved greater than ninety percent gene transfection in HeLa cells and HEK293 cells [ 130 , 131 , 132 , 133 ].…”

Section: Dendrimer Nanoparticulate Ddsmentioning

confidence: 99%

Functionalization of Nanoparticulate Drug Delivery Systems and Its Influence in Cancer Therapy

et al. 2022

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Research into the application of nanocarriers in the delivery of cancer-fighting drugs has been a promising research area for decades. On the other hand, their cytotoxic effects on cells, low uptake efficiency, and therapeutic resistance have limited their therapeutic use. However, the urgency of pressing healthcare needs has resulted in the functionalization of nanoparticles’ (NPs) physicochemical properties to improve clinical outcomes of new, old, and repurposed drugs. This article reviews recent research on methods for targeting functionalized nanoparticles to the tumor microenvironment (TME). Additionally, the use of relevant engineering techniques for surface functionalization of nanocarriers (liposomes, dendrimers, and mesoporous silica) and their critical roles in overcoming the current limitations in cancer therapy—targeting ligands used for targeted delivery, stimuli strategies, and multifunctional nanoparticles—were all reviewed. The limitations and future perspectives of functionalized nanoparticles were also finally discussed. Using relevant keywords, published scientific literature from all credible sources was retrieved. A quick search of the literature yielded almost 400 publications. The subject matter of this review was addressed adequately using an inclusion/exclusion criterion. The content of this review provides a reasonable basis for further studies to fully exploit the potential of these nanoparticles in cancer therapy.

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“…77 Fluorinated dendrimers and polymeric NPs exhibited better NA delivery in serum due to potent endosomal disruption caused by serum protein adsorption resistance. [78][79][80] Achieve High Loading Efficiency and Controlled Drug Release…”

Section: Improve Delivery and Promote Lysosome Escapementioning

confidence: 99%

Improved Nucleic Acid Therapy with Advanced Nanoscale Biotechnology

Weng

Huang

et al. 2020

Molecular Therapy - Nucleic Acids

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Due to a series of systemic and intracellular obstacles in nucleic acid (NA) therapy, including fast degradation in blood, renal clearance, poor cellular uptake, and inefficient endosomal escape, NAs may need delivery methods to transport to the cell nucleus or cytosol to be effective. Advanced nanoscale biotechnology-associated strategies, such as controlling the particle size, charge, drug loading, response to environmental signals, or other physical/chemical properties of delivery carriers, have provided great help for the in vivo and in vitro delivery of NA therapeutics. In this review, we introduce the characteristics of different NA modalities and illustrate how advanced nanoscale biotechnology assists NA therapy. The specific features and challenges of various nanocarriers in clinical and preclinical studies are summarized and discussed. With the help of advanced nanoscale biotechnology, some of the major barriers to the development of NA therapy will eventually be overcome in the near future.

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Self‐Assembled Fluorodendrimers Combine the Features of Lipid and Polymeric Vectors in Gene Delivery

Cited by 24 publications

References 44 publications

Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy

Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy

Functionalization of Nanoparticulate Drug Delivery Systems and Its Influence in Cancer Therapy

Improved Nucleic Acid Therapy with Advanced Nanoscale Biotechnology

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