Self‐assembled Cupric Oxide Nanoclusters for Highly efficient chemodynamic therapy

Abstract

Chemodynamic therapy (CDT) based on Fenton and Fenton-like reactions induces cancer cell killing via in situ catalyzing H 2 O 2 and generating highly oxidative hydroxyl radicals ( * OH) in tumor sites. Their application is not limited by tumor grown depth or hypoxic microenvironment. However, the reaction efficiency is still hampered due to the structure of catalytic agents and the requirement for low pH environment. Here, we design a porous CuO nanocluster (CuO NC) through self-assembly of oleylamine stabilized CuO NPs (OAmÀ CuO NPs), and functionalize it with folic acid (CuO NC-FA) for specific tumor cell targeting. It can catalyze H 2 O 2 with high efficiency in nearly neutral environment. Besides, the porous structure of CuO NC also helps the diffusion of H 2 O 2 to the interior of nanocluster to further improve Fentonlike reaction efficiency. The convenient synthesis of CuO NC-FA with good Fenton-like reaction efficiency at neutral environment demonstrates good chemodynamic therapy effect.

NIR-II light powered hydrogel nanomotor for intravesical instillation with enhanced bladder cancer therapy

“…1B, H 2 O 2 , Cu 2+ ). Cu 2+ reacted with H 2 O 2 via a Fenton-like reaction, 44,45 and the as generated ˙OH was confirmed using the methylene blue (MB) assay. Co-incubating MB with Cu 2+ and H 2 O 2 resulted in a rapid decrease of MB characteristic absorption at 664 nm (Fig.…”

“…Released Cu 2+ catalyzed over-expressed H 2 O 2 intra-bladder cancer cells into • OH via the Fenton-like reaction, and induced cell apoptosis. To evaluate intracellular • OH generation, DCFH-DA, a fluorescent probe which is oxidized by ROS to generate DCF with green fluorescence, 44 was incubated with LipGel-NMs treated MB49 cells, which showed intense intracellular DCF fluorescence, whereas negligible intracellular DCF fluorescence was observed for MB49 cells that treated with NC-LipGel-NMs that did not contain CuO 2 (Fig. 3A, LipGel-NMs, NC-LipGel-NMs).…”

Porous nanomaterials for biosensing and related biological application in in vitro/vivo usability

“…Subsequently, they were modified with folic acid (FA) to target cancer cells, and the obtained CuO NCs-FA were delivered to HeLa cells to verify the feasibility of intracellular Fenton-like reactions. 136 In addition, polydopamine-modified CuO combined with natural glucose oxidase (GOD), in which CuO maximizes the cascade catalytic efficiency with a high loading rate (47.1%) due to the elaborate hollow mesoporous structure (Fig. 10b), was combined with NIR-II-induced thermal effects for combined O 2 -promoted starvation and photothermal-chemical kinetic treatment.…”

Biocompatible Copper-Based Nanocomposites for Combined Cancer Therapy

“…Specifically, CuO is commonly used in CDT to induce intracellular ·OH generation for tumor cell eradication. For example, Jin et al successfully self-assembled CuO nanoparticles (NPs) with an average size of 5 nm into CuO nanospheres (Figure a) through a surfactant-assisted method . The as-obtained CuO nanospheres showed a porous structure that provided a significantly large specific surface area (395.5 m 2 g –1 ), which enhanced active reaction sites and improved Fenton-like reaction efficiency.…”