Self-assembled copper nanoclusters used to mimic peroxidase for glucose detection

Abstract

A sensing system for glucose derived from a self-assembled copper nanoclusters-based nano-enzyme and glucose oxidase.

Gemini Surfactant-Induced Cysteine-Capped Copper Nanoclusters Self-Assembly with Enhanced Peroxidase-Like Activity and Colorimetric Glutathione Sensing

“…28−30 Cu nanoparticles exhibited remarkable enzymatic catalysis when used in the oxidation of o-phenylenediamine (OPD) in the presence of hydrogen peroxide (H 2 O 2 ). 31 Through the assessment of the enzymelike performance of copper nanoclusters, thymidine emerged as a potent template for inducing enzyme-mimicking attributes in these nanoclusters. 32 The successful application of nanozymes in biosensing primarily hinges on their peroxidase activity, involving the oxidation of substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) or 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in the presence of H 2 O 2 , resulting in a noticeable and characteristic color alteration.…”

“…This setup had been utilized to create a fluorescent sensor for detecting cysteine . Copper nanoclusters in their natural condition show amazing tetraenzyme-like characteristics, resembling superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbic acid oxidase (AAO). − Cu nanoparticles exhibited remarkable enzymatic catalysis when used in the oxidation of o-phenylenediamine (OPD) in the presence of hydrogen peroxide (H 2 O 2 ) . Through the assessment of the enzyme-like performance of copper nanoclusters, thymidine emerged as a potent template for inducing enzyme-mimicking attributes in these nanoclusters …”