Self-assembled collagen–human mesenchymal stem cell microspheres for regenerative medicine

Chan, Barbara P.; Hui, T.Y.; Yeung, Celine; Li, J.; Mo, Irene Fung Ying; Chan, Godfrey Chi Fung

doi:10.1016/j.biomaterials.2007.07.041

Cited by 161 publications

(139 citation statements)

References 51 publications

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“…Collagen is a natural biocompatible and biodegradable material [126] and can be reconstituted into fibrous structures simulating the native ECM in tissues. Recently, a microencapsulation system immobilizing living cells within reconstituted collagen fiber meshwork has been established [19] and the collagen meshwork is able to provide a bio-mimetic scaffold supporting cell growth, migration [21], therapeutic protein secretion [122] and stem cell differentiation [52]. Moreover, chemical approaches have been used to design self-assembled peptides [39,128] and these biomimetic peptides can also be used to entrap cells [117].…”

Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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Section: Scaffolding Approaches In Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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“…[1][2][3][4][5][6] For instance, collagen alone accounts for *30% of all vertebrate body proteins and more than 90% of the extracellular proteins in connective tissues. 7 The use of collagen-based scaffolds has been shown to be advantageous because of their inherent cell-binding sites, availability, accessibility, and structural strength.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Chung

Chien

Aguado

et al. 2013

Tissue Engineering Part A

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We report here the use of novel self-assembling collagen-hyaluronic acid (HyA) membranes to deliver bone morphogenetic protein-2 (BMP-2) for orthopedic applications. Prior work has demonstrated that collagen-HyA membranes are formed initially through electrostatic interactions between the oppositely charged collagen and HyA molecules, and that membrane growth is driven by osmotic pressure imbalances between the collagen and HyA solutions. The purpose of this study was to investigate the potential of incorporating charged growth factors such as BMP-2 within the membrane for regenerative medicine applications. Membrane material properties, protein mass loss, and release kinetics of BMP-2, as well as biocompatibility and osteogenic potential in vitro and in vivo using a subcutaneous mouse model were assessed. Scanning electron microscopy and mechanical testing confirmed no loss of structural or mechanical integrity upon BMP-2 incorporation into the membranes. Slow and steady release of the growth factor was demonstrated with 17% of total loaded BMP-2 released over the course of 49 days. To test biocompatibility and osteogenic potential in vitro, human mesenchymal stem cells were cultured on collagen-HyA membranes and showed greater proliferation rates (for up to 28 days) on membranes without BMP-2, but a greater alkaline phosphatase activity and osteocalcin production on membranes releasing BMP-2. In vivo subcutaneous implantation of the membranes showed a minimal immune response with osteoblasts and mineral deposits present in the ectopic site for BMP-2-releasing membranes, further demonstrating the potential of the BMP-2-releasing membranes to induce osteogenic differentiation. This study presents a novel strategy to create self-assembled membranes using two biocompatible molecules that can deliver bioactive agents in a sustained manner to induce a local regenerative response.

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“…Biomatrices with tailored mechanical properties, incorporated exogenous growth factors, or specific functional groups conjugated to synthetic polyethylene glycol (PEG) polymer chains have been successfully used to guide MSC fate down specific differentiation pathways [5,6]. However, assessment of MSC multidifferentiation potential has primarily been limited to MSCs cultured on two-dimensional substrates or has been applied after MSCs have grown out of or been chemically removed from the supporting biomatrix [7][8][9]. Moreover, little is known regarding monocyte/macrophage (Mø) influence on MSC fate when encapsulated within a three-dimensional biomatrix, which is an important interaction that must be considered when delivering MSCs to the inflammatory milieu of injured or diseased tissues.…”

Section: Introductionmentioning

confidence: 99%

Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype

Cantu

Hematti

Kao

2012

Stem Cells Translational Medicine

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Self-assembled collagen–human mesenchymal stem cell microspheres for regenerative medicine

Cited by 161 publications

References 51 publications

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype

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