Self-assembled chitosan nanoparticles for intranasal delivery of recombinant protein interleukin-17 receptor C (IL-17RC): preparation and evaluation in asthma mice

Li, Yongli; Zhang, Jianhua; Wang, Chaoying

doi:10.1080/21655979.2021.1940622

Cited by 10 publications

(10 citation statements)

References 46 publications

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“…Afterward, the nanoparticles were labeled by RAI 131. Chitosan is a nano-carrier with numerous advantages, such as good biocompatibility, nontoxicity, and ease of preparation; its absorption-enhancing properties make it an ideal carrier for the delivery of genes [ 26 ]. Therefore, the vector we constructed can achieve the anti-tumor effect from two aspects.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Chang

Ling

et al. 2021

Bioengineered

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Differentiated thyroid cancer (DTC), such as papillary thyroid cancer, has a good prognosis after routine treatment. However, in the course of treatment, 5% to 20% of cases may dedifferentiate and can be transformed into dedifferentiated DTC (deDTC) or anaplastic thyroid cancer, leading to treatment failure. To date, several drugs have been used effectively for dedifferentiated thyroid cancer, whereas gene therapy may be a potential method. Literature reported that double suicide genes driven by human telomerase reverse transcriptase promoter (hTERTp) can specifically express in cancer cells and kill them. However, the weak activity of hTERTp limits its further research. To overcome this weakness, we constructed a novel chitosan nanocarrier containing double suicide genes driven by a 'gene switch' (a cascade of radiation enhancer E9 and a hTERTp). The vector was labeled with iodine-131 ( 131 I). On one hand, E9 can significantly enhance the activity of hTERTp under the weak radiation of 131 I, thereby increasing the expression of double suicide genes in deDTC cells. On the other hand, 131 I also plays a certain killing role when it enters host cells. The proposed nanocarrier has good specificity for deDTC cells and thus deserves further study.

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Section: Discussionmentioning

confidence: 99%

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Chang

Ling

et al. 2021

Bioengineered

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“…100 µL of stop solution was then added to each well to stop the reaction. Absorbance at 450 nm was recorded to calculate the concentration of target proteins [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Everolimus ameliorates Helicobacter pylori infection-induced inflammation in gastric epithelial cells

Liu

Zhang

et al. 2022

Bioengineered

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Helicobacter pylori ( H.pylori ) infection caused by gastric mucosal inflammation plays a pivotal role in the progression of gastric diseases. The recruitment and attachment of monocytes to the gastric mucosal epithelium are a major event in the early stages of H. pylori -associated gastric diseases. Everolimus is a mechanistic/mammalian target of rapamycin (mTOR) inhibitor used to prevent tumor growth by inhibiting the PI3K signaling pathway. Here, we examined the pharmacological role of Everolimus against H.pylori- induced damage in gastric epithelial cells. Firstly, we found that Everolimus ameliorated H.pylori- induced oxidative stress by reducing reactive oxygen species (ROS) and malondialdehyde (MDA). Secondly, Everolimus significantly reduced the expressions of the pro-inflammatory cytokines interleukin (IL)-6, tumor necrosis factor-α (TNF-α), and IL-8. Moreover, it decreased the production of the pro-inflammatory chemokines C-X-C motif ligand 1 (CXCL1) and macrophage chemoattractant protein-1 (MCP-1). Importantly, Everolimus suppressed the induction of the adhesion molecule intracellular adhesion molecule-1 (ICAM-1) and the attachment of THP-1 monocytes to gastric epithelial AGS cells. Our data also shows that Everolimus inhibited the activation of the NF-κB signaling pathway. Therefore, we conclude that Everolimus could protect gastric epithelial cells by mitigating H.pylori- induced inflammatory response and the attachment of monocytes to epithelial cells.

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“…In virtue of their biodegradability, biocompatibility, low toxicity, mucoadhesion, large surface area-to-volume ratio, good drug payload, controlled release and permeation enhancer properties, CS-based micro- and nanoparticles were profitably utilized for improving the effectiveness of nasal administration of several kinds of active agents, including antihypertensive [ 123 ], antiemetic [ 124 , 125 ], anti-asthmatic [ 126 , 127 , 128 ], antibiotic [ 129 ] or anti-inflammatory [ 130 , 131 ] drugs. The favorable properties of CS micro- and nanoparticulate systems can allow a reduction in the dose and frequency of drug administration and, consequently, its side effects.…”

Section: Chitosan-based Mucosal Drug Delivery Systemsmentioning

confidence: 99%

Multiple Roles of Chitosan in Mucosal Drug Delivery: An Updated Review

et al. 2022

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Chitosan (CS) is a linear polysaccharide obtained by the deacetylation of chitin, which, after cellulose, is the second biopolymer most abundant in nature, being the primary component of the exoskeleton of crustaceans and insects. Since joining the pharmaceutical field, in the early 1990s, CS attracted great interest, which has constantly increased over the years, due to its several beneficial and favorable features, including large availability, biocompatibility, biodegradability, non-toxicity, simplicity of chemical modifications, mucoadhesion and permeation enhancer power, joined to its capability of forming films, hydrogels and micro- and nanoparticles. Moreover, its cationic character, which renders it unique among biodegradable polymers, is responsible for the ability of CS to strongly interact with different types of molecules and for its intrinsic antimicrobial, anti-inflammatory and hemostatic activities. However, its pH-dependent solubility and susceptibility to ions presence may represent serious drawbacks and require suitable strategies to be overcome. Presently, CS and its derivatives are widely investigated for a great variety of pharmaceutical applications, particularly in drug delivery. Among the alternative routes to overcome the problems related to the classic oral drug administration, the mucosal route is becoming the favorite non-invasive delivery pathway. This review aims to provide an updated overview of the applications of CS and its derivatives in novel formulations intended for different methods of mucosal drug delivery.

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Self-assembled chitosan nanoparticles for intranasal delivery of recombinant protein interleukin-17 receptor C (IL-17RC): preparation and evaluation in asthma mice

Cited by 10 publications

References 46 publications

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Everolimus ameliorates Helicobacter pylori infection-induced inflammation in gastric epithelial cells

Multiple Roles of Chitosan in Mucosal Drug Delivery: An Updated Review

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