Self-assembled cell sheets composed of mesenchymal stem cells and gelatin nanofibers for the treatment of full-thickness wounds

Pham-Nguyen, Oanh-Vu; Shin, Ji Un; Kim, Hyesung; Yoo, Hyuk Sang

doi:10.1039/d0bm00910e

Cited by 22 publications

(16 citation statements)

References 43 publications

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“…We previously prepared hydronanofibrous meshes by two different strategies: (1) a bottom-up strategy of decorating surfaces after electrospinning and (2) a top-down strategy of eroding surfaces to obtain required surface properties after electrospinning. The “bottom-up” strategy was employed to deposit gelatin on the surfaces of nanofibers to obtain a high degree of cell viability, , “top-down” approach was employed for alginate nanofibrous meshes where the core alginate of the PCL/alginate coaxial nanofiber was cross-linked and the PCL shell was discarded . Thus, we herein investigated the “top-down” strategy of gelatin nanofibers using PCL/gelatin coelectrospinning.…”

Section: Resultsmentioning

confidence: 99%

“…To facilitate the surface-etching process of P@G nanofibers, we decided to fix the PCL flow rate at 1.5 mL/h for the rest of the study. On the other hand, we previously examined the morphology of the PCL nanofibrils. ,, After the solvent treatment, the PCL shell was supposed to be thinner. However, as we examined the core–shell structure of coaxial electrospun nanofibers in our previous study using a transmission electron microscope (TEM), the PCL shell itself was confirmed to be very thin (<5 nm).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, water-soluble polymers are generally blended with poly(vinyl alcohol) (PVA) or poly(ethylene oxide) for better electrospinnability. To overcome this issue, we performed coaxial electrospinning with an inner and an outer needle so that the two immiscible solvents could be coejected and electrospun to form fibrous meshes. , In our previous study, we employed PCL as a sacrificial layer to maintain a fibrous morphology until the gelatin inner core was fully cross-linked with glutaraldehyde (GTA). We observed that the fibrous morphology was well maintained during the process and confirmed the viscoelastic properties of the cross-linked gelatin in the nanofibers.…”

Section: Introductionmentioning

confidence: 99%

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Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

2020

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Electrospun nanofibers are widely employed as cell culture matrices because their biomimetic structures resemble a natural extracellular matrix. However, due to the limited cell infiltration into nanofibers, three-dimensional (3D) construction of a cell matrix is not easily accomplished. In this study, we developed a method for the partial digestion of a nanofiber into fragmented nanofibers composed of gelatin and polycaprolactone (PCL). The PCL shells of the coaxial fragments were subsequently removed with different concentrations of chloroform to control the remaining PCL on the shell. The swelling and exposure of the gelatin core were manipulated by the remaining PCL shells. When cells were cultivated with the fragmented nanofibers, they were spontaneously assembled on the cell sheets. The cell adhesion and proliferation were significantly affected by the amount of PCL shells on the fragmented nanofibers.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

2020

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“…Porous gelatin matrices absorb wound exudates and maintain moisture, thus promoting the wound healing process. Gelatin-based dressings act as porous materials for cell migration and offer mechanical and structural support for the development of new tissue [ 30 ]. Although gelatin is a promising biopolymer employed as a wound dressing material, it has no antibacterial efficacy to prevent wound infections or bacterial invasion of the wound [ 31 ].…”

Section: Properties Of Gelatin In Wound Dressing Applicationsmentioning

confidence: 99%

Gelatin-Based Hybrid Scaffolds: Promising Wound Dressings

et al. 2021

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Wound care is a major biomedical field that is challenging due to the delayed wound healing process. Some factors are responsible for delayed wound healing such as malnutrition, poor oxygen flow, smoking, diseases (such as diabetes and cancer), microbial infections, etc. The currently used wound dressings suffer from various limitations, including poor antimicrobial activity, etc. Wound dressings that are formulated from biopolymers (e.g., cellulose, chitin, gelatin, chitosan, etc.) demonstrate interesting properties, such as good biocompatibility, non-toxicity, biodegradability, and attractive antimicrobial activity. Although biopolymer-based wound dressings display the aforementioned excellent features, they possess poor mechanical properties. Gelatin, a biopolymer has excellent biocompatibility, hemostatic property, reduced cytotoxicity, low antigenicity, and promotes cellular attachment and growth. However, it suffers from poor mechanical properties and antimicrobial activity. It is crosslinked with other polymers to enhance its mechanical properties. Furthermore, the incorporation of antimicrobial agents into gelatin-based wound dressings enhance their antimicrobial activity in vitro and in vivo. This review is focused on the development of hybrid wound dressings from a combination of gelatin and other polymers with good biological, mechanical, and physicochemical features which are appropriate for ideal wound dressings. Gelatin-based wound dressings are promising scaffolds for the treatment of infected, exuding, and bleeding wounds. This review article reports gelatin-based wound dressings which were developed between 2016 and 2021.

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“…Originally derived from mesoblast, adipose tissue-derived stem cells (ADSCs) are easily accessible with minimal invasiveness, which harbor the ability of self-renewal and multilineage potential, differentiating into keratinocytes, broblasts, and angiogenic cells [1,2]. A large number of studies have indicated that implantation of ADSCs shows promising properties in wound healing [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

miR-466 Regulates the Migration of ADSCs in Repairing Cutaneous Wound via Targeting SCF/c-kit/ERK Signaling Axis

Xia

Zhang

Jin

et al. 2021

Preprint

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BackgroundWound healing is a complex process and the treatment of chronic non-healing wounds still remains a tough challenge. Mesenchymal stem cell (MSC) based therapy has emerged as a promising therapeutic strategy for wound healing. Stem cell factor/stem cell factor receptor (SCF/c-kit) interaction activates downstream signal pathways in stem cells. microRNAs (miRNAs) regulate the effect of adipose tissue-derived stem cells (ADSCs) in cutaneous wound, but the associated cellular mechanisms are not fully understood. In the research, we investigate the inhibitory effect of miR-466 in ADSC migration induced by SCF/c-kit/ERK axis.MethodsHuman ADSCs were tested by scratch assay and Transwell assay to evaluate the migration ability. MicroRNA sequencing was used to find the discrepancy miRNA in ADSCs with or without SCF pretreatment. The ERK signaling pathway was investigated by pharmacological disruption of PD98059. In addition, siRNA, RT-qPCR, western blotting, and luciferase reporter assay were used to investigate the mechanism of miR-466 regulating migration via the SCF/c-kit/ERK pathway. In the in vivo study, the wound healing ability of miR-466 and SCF was evaluated in a nude mouse full-thickness skin wound model.ResultsADSCs incubated with SCF induces the expression of c-kit and promotes ADSC migration, whereas siRNA c-kit inhibited the effects. microRNAs microarray showed that miR-466 was upregulated in SCF pretreatment ADSCs compared to ADSCs. The luciferase reporter assay indicated that miR-466 may target c-kit and suppress SCF/c-kit-mediated migration of ADSCs. The ERK signaling pathway was confirmed to be involved in SCF/c-kit induced migration. In addition, pre-treatment with PD98059 reversed the effects of SCF/c-kit on the ERK signaling and migration-related proteins. Furthermore, miR-466 inhibits ADSC migration to wound site and defects cutaneous wound healing in vivo.ConclusionsTaken together, our data suggested that SCF/c-kit signal axis can enhance the activation of ERK signal pathway, which will promote ADSC migration. miR-466 regulates ADSC migration by targeting SCF/c-kit/ERK signaling and defects wound healing.

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Self-assembled cell sheets composed of mesenchymal stem cells and gelatin nanofibers for the treatment of full-thickness wounds

Abstract: Gelatin-layered PCL nanofibrils for 3D cell sheet formation were composed with adipocyte-derived stem cells for wound healing.

Cited by 22 publications

References 43 publications

Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

Gelatin-Based Hybrid Scaffolds: Promising Wound Dressings

miR-466 Regulates the Migration of ADSCs in Repairing Cutaneous Wound via Targeting SCF/c-kit/ERK Signaling Axis

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