Selenoprotein K protects skeletal muscle from damage and is required for satellite cells-mediated myogenic differentiation

Wang, Shengchen; Zhao, Xiaona; Liu, Qingqing; Wang, Yue; Li, Shu; Xu, Shiwen

doi:10.1016/j.redox.2022.102255

Cited by 55 publications

(32 citation statements)

References 67 publications

(71 reference statements)

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“…It can promote DHHC6-catalysed palmitoylation of CD36, which is required for macrophage function [ 8 , 29 , 41 , 42 ]. In addition, SELENOK plays an important role in the differentiation of myogenic cells mediated by satellite cells [ 43 ], and the degradation of SELENOK is required for adipocyte differentiation [ 44 ]. The effects of SELENOK-mediated palmitoylation on DCs’ function and SELENOK deficiency on DCs’ differentiation and maturation are worthy of future investigation.…”

Section: Discussionmentioning

confidence: 99%

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells

et al. 2022

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Selenoprotein K (SELENOK) is an endoplasmic reticulum stress (ERS)-regulated protein required for the calcium (Ca2+) flux-mediated migration of T cells and neutrophils, and the migration and phagocytosis of macrophages and microglia. However, the effect of SELENOK on the regulation of the immune function of dendritic cells (DCs), including immature DCs (imDCs) and mature DCs (mDCs), is still unclear. In this study, imDCs prepared from SELENOK knockout mice were used to evaluate the effect of SELENOK on the migration and phagocytosis of imDCs. The results showed that ERS-induced downregulation of imDCs phenotypic markers led to a reduction in Ras homolog gene family member A (RhoA)-dependent migration and enhanced Ca2+/CD205-mediated phagocytosis. SELENOK deficiency-induced upregulation of selenoprotein S (SELENOS) attenuated ERS levels in imDCs. An increase in Ca2+ levels resulted in increased migration and decreased phagocytosis with or without ERS conditions. The migration was RhoA-dependent, and Ca2+ or CD205 was associated with regulating phagocytosis in imDCs. Our study found that SELENOK is required for imDC migration and phagocytosis.

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Section: Discussionmentioning

confidence: 99%

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells

et al. 2022

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“…However, the cell has three levels of protection against these free radicals [9][10][11], the first one is represented by enzymes that have antioxidant activity such as catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). These enzymes are detoxify free radicals at an early stage of formation [12]. Furthermore, metal-binding proteins are considered in the first level of antioxidant defense, where free iron and copper are the most important catalyzers of free radical formation.…”

Section: Introductionmentioning

confidence: 99%

Selenium and/or vitamin E upregulate the antioxidant gene expression and parameters in broilers

et al. 2022

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Background In contrast to free radicals, the first line of protection is assumed to be vitamin E and selenium. The present protocol was designed to assess the roles of vitamin E and/or a selenium-rich diet that affected the blood iron and copper concentrations, liver tissue antioxidant and lipid peroxidation, and gene expression linked to antioxidants in the liver tissue of broilers. The young birds were classified according to the dietary supplement into four groups; control, vitamin E (100 mg Vitamin/kg diet), selenium (0.3 mg sodium selenite/kg diet), and vitamin E pulse selenium (100 mg vitamin/kg diet with 0.3 mg sodium selenite/kg diet) group. Results The results of this experiment suggested that the addition of vitamin E with selenium in the broiler diet significantly increased (P ≤ 0.05) serum iron when compared with the other groups and serum copper when compared with the vitamin E group. Moreover, the supplements (vitamin E or vitamin E with selenium) positively affected the enzymatic activity of the antioxidant-related enzymes with decreased malondialdehyde (MDA),which represents lipid peroxidation in broiler liver tissue. Moreover, the two supplements significantly upregulated genes expression related to antioxidants. Conclusion Therefore, vitamin E and/or selenium can not only act as exogenous antioxidants to prevent oxidative damage by scavenging free radicals and superoxide, but also act as gene regulators, regulating the expression of endogenous antioxidant enzymes.

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“…In our study, MT alleviated excessive generation of ROS and mitochondrial dysfunction in ST cells caused by TBBPA. Previous studies reported the increase of ROS level was pivotal in the induction of cell death and caused different types of cell death through activation of different signaling pathways 42,43 . PI3K/AKT signaling pathway can be activated by various types of cellular stimulation or toxic stimulation to regulate basic cellular functions.…”

Section: Discussionmentioning

confidence: 99%

The antagonistic effect of melatonin onTBBPA‐induced apoptosis and necroptosis viaPTEN/PI3K/AKTsignaling pathway in swine testis cells

Sun

Wang

Zhang

et al. 2022

Environmental Toxicology

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Tetrabromobisphenol A (TBBPA) is a widely used industrial brominated flame retardant, which can endanger animal and human health, including cytotoxicity, endocrine disruption, reproductive toxicity and so on. Melatonin (MT) is a noteworthy free radical scavenger and an antioxidant to alleviate oxidative stress. To investigate the cytotoxic of TBBPA on swine testis cells (ST cells), as well as the antagonistic effect of MT, we established TBBPA exposure and MT antagonistic models, used flow cytometry and AO/EB staining methods to detect apoptosis and necroptosis, used DCFH-DA method to examine the content of reactive oxygen species (ROS) and investigated the expression of associated genes using RT-PCR and Western blot. According to our findings, TBBPA exposure induced cell death in ST cells. TBBPA increased ROS levels, thus increasing PTEN expression and decreasing PI3K and AKT expression. Apoptosis-related factors (Caspase-3, Bax, Cyt-c, and Caspase-9) and necroptosis-related factors (RIPK1, RIPK3, and MLKL) were considerably elevated, in addition to the reduced expression of BCL-2 and Caspase-8. We also found that MT inhibited apoptosis and necroptosis in TBBPA-induced ST cells and effectively resolved the abnormal expression of related signaling pathways. In summary, the above results indicate that MT alleviates the disorder of PTEN/PI3K/AKT signaling pathway via inhibiting ROS overproduction, thereby mitigating apoptosis and necroptosis caused by TBBPA. This research provides a theoretical basis for further understanding of the toxicity of TBBPA and the detoxification of MT against environmental toxics.

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Selenoprotein K protects skeletal muscle from damage and is required for satellite cells-mediated myogenic differentiation

Cited by 55 publications

References 67 publications

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells

Selenium and/or vitamin E upregulate the antioxidant gene expression and parameters in broilers

The antagonistic effect of melatonin onTBBPA‐induced apoptosis and necroptosis viaPTEN/PI3K/AKTsignaling pathway in swine testis cells

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