Selenium uptake and assessment of the biochemical changes inArthrospira(Spirulina)platensisbiomass during the synthesis of selenium nanoparticles

Zinicovscaia, Inga; Chiriac, Tatiana; Cepoi, Liliana; Rudi, Ludmila; Culicov, Otilia; Frontasyeva, M. V.; Valeriu, Rudic

doi:10.1139/cjm-2016-0339

Cited by 20 publications

(10 citation statements)

References 31 publications

(27 reference statements)

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“…Our findings were in accordance with the study results of Choudhary et al (2007) , Sadiq et al (2011) and Suman, Rajasree & Kirubagaran (2015) , where the treatement of ZnO NPs showed time- and dose-dependent growth inhibition of S. platensis by Zn ions, and C. vulgaris by aluminum oxide NPs and ZnO NPs respectively. Besides, our results showed a substantial reduction in all studied cytotoxicity parameters at an exposure time of 48 h. A similar finding was reported in reduction of phycocyanin from S. platensis by selenium ions ( Zinicovscaia et al, 2016 ), this may be due to highest dissolution of zinc ions from ZnO NPs at 48 h which was recently reported by Bhuvaneshwari et al (2015) .…”

Section: Discussionsupporting

confidence: 91%

“…Our findings were similar to previous studies on S. platensis which reported 93.5% and 50% reduction in Chl-a and carotenoids respectively on day 10 with 10 mg/L of ZnO NPs ( Lone et al, 2013 ), and 53.1%, 37.5% and 32.1% reduction in Chl-a, carotenoids and phycocyanin by the salt stress of sea water on day 25 ( Leema et al, 2010 ). A recent study by Zinicovscaia et al (2016) reported 90% loss in phycocyanin pigment of S. platensis after 72 h of contact time with 100 mg/L of selenium ions.…”

Section: Discussionmentioning

confidence: 98%

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Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

Djearamane

Lim

Wong

et al. 2018

PeerJ

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BackgroundThe extensive usage of zinc oxide nanoparticles (ZnO NPs) in industrial and consumer products raises the risk of releasing their residues into the aquatic environment. The presence of ZnO NPs in the aquatic environment could potentially cause cytotoxic effects on aquatic organisms. Thus, investigating the cytotoxic effects of ZnO NPs on microalgae, which form the base for the food web of aquatic biota, is essential to gain information regarding the ecotoxicological effects of metallic oxide nanoparticles in the aquatic ecosystem. Therefore, the present study has investigated in detail the assorted cytotoxic effects of ZnO NPs on S. platensis using various concentrations of ZnO NPs (10–200 mg/L) from 6 to 96 h to explore the dose- and time-dependent cytotoxic effects.MethodsThe cytotoxic effects were all assessed through quantification of loss in cell viability, reduction in biomass and decrease in photosynthetic pigments such as chlorophyll-a, carotenoids and phycocyanin. The surface interactions of nanoparticles and the subsequent morphological alterations on algal cells were examined by optical and scanning electron microscopy (SEM). The intracellular alterations of algal cells were studied using transmission electron microscopy. Furthermore, Fourier transformed infrared (FTIR) spectrum was obtained to investigate the involvement of algal surface biomolecules in surface binding of ZnO NPs on algal cells.ResultsThe treatment of ZnO NPs on S. platensis exhibited a typical concentration- and time-dependent cytotoxicity. Results showed a significant (p < 0.05) cytotoxicity from 24 h onwards for all tested concentrations of ZnO NPs. The maximum cytotoxicity on algal cells was achieved at 96 h of exposure to ZnO NPs. In comparison with control, the algal cells that interacted with 200 mg/L of ZnO NPs for 96 h showed 87.3 ± 1% loss in cell viability, 76.1 ± 1.7% reduction in algal biomass, 92.5 ± 2.2%, 76.2 ± 2.2% and 74.1 ± 3.4% decrease in chlorophyll-a, carotenoids and phycocyanin contents respectively. Our study confirmed the cytotoxicity of ZnO NPs through the algal growth inhibition with 72 h EC10 and EC50 values of 1.29 and 31.56 mg/L, respectively. The microscopic examinations of the algal cells that interacted with ZnO NPs showed severe cell membrane and intracellular damage. The SEM EDX spectrum of ZnO NPs treated algal biomass evidenced the surface accumulation of zinc in the biomass. Finally, the FTIR spectrum confirmed the involvement of amino, hydroxyl and carboxylic groups of algal cell wall in the surface interaction of ZnO NPs on the algal cells.DiscussionThe results showed that the treatment of ZnO NPs on S. platensis triggered substantial cytotoxicity and caused cell death. Hence, S. platensis could be potentially used as a bioindicator for testing toxicity of ZnO NPs in aquatic environment.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

Djearamane

Lim

Wong

et al. 2018

PeerJ

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“…2C and 2D). Similar results were reported by Dmytryk, Saeid & Chojnacka (2014), Zinicovscaia et al (2016), and Djearamane et al (2018) who demonstrated the cellular accumulation of selenium, zinc and ZnO NPs in the algal biomass of Spirulina platensis through EDX analysis. Furthermore, the quantification of ZnO NPs accumulated in algal cells using ICP OES exhibited a dose- and time-dependent increase in cellular accumulation of ZnO NPs in H. pluvialis with highest zinc accumulation of 6.27–18.36 pg/cell (Fig.…”

Section: Discussionsupporting

confidence: 89%

Cellular accumulation and cytotoxic effects of zinc oxide nanoparticles in microalga Haematococcus pluvialis

Djearamane

Lim

Wong

et al. 2019

PeerJ

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Background Zinc oxide nanoparticles (ZnO NPs) are widely used in household and cosmetic products which imply an increased releasing of these particles into the environment, especially aquatic ecosystems, resulting in the need of assessing the potential toxic effects of ZnO NPS on the aquatic organisms, particularly on microalgae which form the base for food chain of aquatic biota. The present study has investigated the dose- and time-dependent cellular accumulation and the corresponding cytotoxic effects of increasing concentrations of ZnO NPs from 10–200 μg/mL on microalga Haematococcus pluvialis at an interval of 24 h for 96 h. Methods The scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) was used to qualitatively detect the cellular accumulation of ZnO NPs in algal cells, while inductively coupled plasma optical emission spectrometry (ICP OES) was performed to quantify the cell associated-zinc in algal cells. The percentage of cell death, reduction in algal biomass, and loss in photosynthetic pigments were measured to investigate the cytotoxic effects of ZnO NPs on H. pluvialis. Extracellular and intracellular changes in algal cells resulted from the treatment of ZnO NPs were demonstrated through optical, scanning, and transmission electron microscopic studies. Results SEM-EDX spectrum evidenced the accumulation of ZnO NPs in algal biomass and ICP OES results reported a significant (p < 0.05) dose- and time-dependent accumulation of zinc in algal cells from 24 h for all the tested concentrations of ZnO NPs (10–200 μg/mL). Further, the study showed a significant (p < 0.05) dose- and time-dependent growth inhibition of H. pluvialis from 72 h at 10–200 μg/mL of ZnO NPs. The morphological examinations revealed substantial surface and intracellular damages in algal cells due to the treatment of ZnO NPs. Discussion The present study reported the significant cellular accumulation of ZnO NPs in algal cells and the corresponding cytotoxic effects of ZnO NPs on H. pluvialis through the considerable reduction in algal cell viability, biomass, and photosynthetic pigments together with surface and intracellular damages.

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“…Several types of bacteria and cyanobacteria cultivated in submerged cultures have already been used for the biosynthesis of SeNPs, and these include Escherichia coli [366], Enterobacter cloacae [367], Pseudomonas aeruginosa [368], Klebsiella pneumoniae [369], Zooglea ramigera [370], Lactobacillus casei [371], Streptomyces sp. [372], Arthrospira [Spirulina] platensis [373], and many others. Further, fungal cultures may be used for in vivo synthesis of SeNPs.…”

Section: Encapsulation Of Nanoselenium Particles Into Polysaccharidesmentioning

confidence: 99%

Selenium-Containing Polysaccharides—Structural Diversity, Biosynthesis, Chemical Modifications and Biological Activity

2021

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Selenosugars are a group of sugar derivatives of great structural diversity (e.g., molar masses, selenium oxidation state, and selenium binding), obtained as a result of biosynthesis, chemical modification of natural compounds, or chemical synthesis. Seleno-monosaccharides and disaccharides are known to be non-toxic products of the natural metabolism of selenium compounds in mammals. In the case of the selenium-containing polysaccharides of natural origin, their formation is also postulated as a form of detoxification of excess selenium in microorganisms, mushroom, and plants. The valency of selenium in selenium-containing polysaccharides can be: 0 (encapsulated nano-selenium), IV (selenites of polysaccharides), or II (selenoglycosides or selenium built into the sugar ring to replace oxygen). The great interest in Se-polysaccharides results from the expected synergy between selenium and polysaccharides. Several plant- and mushroom-derived polysaccharides are potent macromolecules with antitumor, immunomodulatory, antioxidant, and other biological properties. Selenium, a trace element of fundamental importance to human health, has been shown to possess several analogous functions. The mechanism by which selenium exerts anticancer and immunomodulatory activity differs from that of polysaccharide fractions, but a similar pharmacological effect suggests a possible synergy of these two agents. Various functions of Se-polysaccharides have been explored, including antitumor, immune-enhancement, antioxidant, antidiabetic, anti-inflammatory, hepatoprotective, and neuroprotective activities. Due to being non-toxic or much less toxic than inorganic selenium compounds, Se-polysaccharides are potential dietary supplements that could be used, e.g., in chemoprevention.

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Selenium uptake and assessment of the biochemical changes inArthrospira(Spirulina)platensisbiomass during the synthesis of selenium nanoparticles

Cited by 20 publications

References 31 publications

Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

Cellular accumulation and cytotoxic effects of zinc oxide nanoparticles in microalga Haematococcus pluvialis

Selenium-Containing Polysaccharides—Structural Diversity, Biosynthesis, Chemical Modifications and Biological Activity

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