Selenium Supplementation Reduced Oxidative DNA Damage in Adnexectomized BRCA1 Mutations Carriers

Abstract

Some experimental evidence suggests that BRCA1 plays a role in repair of oxidative DNA damage. Selenium has anticancer properties that are linked with protection against oxidative stress. To assess whether supplementation of BRCA1 mutation carriers with selenium have a beneficial effect concerning oxidative stress/DNA damage in the present double-blinded placebo control study, we determined 8-oxodG level in cellular DNA and urinary excretion of 8-oxodG and 8-oxoGua in the mutation carriers. We found that 8-oxodG level in leukocytes DNA is significantly higher in BRCA1 mutation carriers. In the distinct subpopulation of BRCA1 mutation carriers without symptoms of cancer who underwent adnexectomy and were supplemented with selenium, the level of 8-oxodG in DNA decreased significantly in comparison with the subgroup without supplementation. Simultaneously in the same group, an increase of urinary 8-oxoGua, the product of base excision repair (hOGG1 glycosylase), was observed. Therefore, it is likely that the selenium supplementation of the patients is responsible for the increase of BER enzymes activities, which in turn may result in reduction of oxidative DNA damage. Importantly, in a double-blinded placebo control prospective study, it was shown that in the same patient groups, reduction in cancer incidents was observed. Altogether, these results suggest that BRCA1 deficiency contributes to 8-oxodG accumulation in cellular DNA, which in turn may be a factor responsible for cancer development in women with mutations, and that the risk to developed breast cancer in BRCA1 mutation carriers may be reduced in selenium-supplemented patients who underwent adnexectomy. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2923-8)

Preserved Expression of mRNA Coding von Willebrand Factor-Cleaving Protease ADAMTS13 by Selenite and Activated Protein C

“…We assessed the role of drugs used in patients with septic shock that promote vasoconstriction (norepinephrine, AVP) (46), of a stabilized analogue of AVP triggering the release of VWF from endothelial cells (DDAVP) (47), selenite supplementation with known antioxidative properties (48) and APC which is reported to regulate severe inflammatory response (49). In HSCs, all tested compounds displayed nonsignificant effects on ADAMTS13 transcription at 6 h ( Figure 2A).…”

Urinary 8-Oxoguanine as a Predictor of Survival in Patients Undergoing Radiotherapy

“…Therefore, increase in urinary excretion of the modified base in the group with longer life expectancy is, most likely, a measure of better response to the radiation (higher radiosensitivity) and simultaneously efficient removal of the damage from cellular DNA of this group (14). Because 8-oxo-Gua is a repair product of the DNA damage, it is likely that, at least in the case of some patients with the higher activity of OGG1 the combination of higher OGG1 repair capacity and irradiation was associated with stable background level of 8-oxo-Gua in cellular DNA.…”

Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

“…Zinc is an essential cofactor of various metalloenzymes and it protects the skin from UV irradiation and has been used as a destructive agent for the management of cancer [7,8]. Sodium selenate enhances the repair system of DNA segments and reduces the risk of new cancer development [9,10]. Numerous literature suggests that it prevents skin cancers in combination with L-selenomethionine [11,12].…”