Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in the HEK293 cells

Özsobacı, Nural Pastacı; Ergün, Dilek Düzgün; Durmuş, Sinem; Tunçdemi̇r, Matem; Uzun, Hafize; Gelışgen, Remise; Özçelik, Derviş

doi:10.1016/j.jtemb.2018.04.008

Cited by 15 publications

(7 citation statements)

References 38 publications

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“…Thus, lead-induced renal oxidative stress is mediated by ROS formation ( Gautam and Flora, 2010 ; Jiang et al., 2018 ). The antioxidant systems in the body can scavenge ROS (e.g., superoxide radicals, hydroxyl radicals, peroxide radicals, nitric oxide)-induced harmful effects, and reset the oxidant-antioxidant equilibrium in blood and organs ( Pastaci et al., 2018 ). An increase in oxidative stress also causes cell death and oxidation of macromolecule ( Sas et al., 2018 ; Bonaterra et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

Shi

Tian

et al. 2020

Front. Pharmacol.

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Lead (Pb) is an important environmental pollutant. Oxidative stress and the inflammatory response have been postulated as mechanisms involved in lead-induced renal damage. Smilax glabra Roxb. has been used for treatment of heavy-metal poisoning in China for 500 years. We investigated S. glabra flavonoids extract (SGF) could attenuate lead acetate-induced nephrotoxicity in weaning rats and human embryonic kidney (HEK)-293 cells, and investigated the possible mechanisms. Compared with Pb exposed group of weaning rats, SGF could significantly promote lead excretion in the blood and kidney, and increase the content of the renal-function indicators blood urea nitrogen, serum uric acid, and serum creatinine. SGF could improve the glomerular filtration rate (GFR) and histologic changes in the kidneys of weaning rats exposed to Pb. SGF could also reduce lead-induced cytotoxicity, improve DNA damage-induced apoptosis and cleaved caspase-3-mediated apoptosis in HEK-293 cells stimulated with Pb. SGF significantly increased the activity of the antioxidant enzymes superoxide dismutase, glutathione peroxidase and catalase, and decreased excessive release of reactive oxygen species (ROS) and malondialdehyde in the kidneys of the weaning rats and in HEK-293 cells. The antioxidant mechanism of SGF related to activation of the Kelch-like ECH-associated protein 1/nuclear-factor-E2-related factor 2/hemeoxygenase-1(Keap1/Nrf2/HO-1) pathway. SGF could inhibit secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α induced by Pb in vivo and in vitro . The anti-inflammatory mechanism of SGF related to inhibition of ROS and pro-inflammatory cytokines triggered the nuclear factor-kappa B (NF-κB) pathway through blockade of inhibitors of I-κB degradation, phosphorylation of NF-κB p65, and nuclear translocation of p65. Our findings indicate that SGF could be a natural antioxidant and anti-inflammatory agent for treating lead-induced nephrotoxicity.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

Shi

Tian

et al. 2020

Front. Pharmacol.

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“…Apoptosis and more ROS was also seen in MCF-7 breast cancer cells after exposure to 217 Hz pulsed 900, 1800, and 2450 MHz RF-EMF (average SAR: 0.36 W/kg) for 1 h [ 111 ]. RF-EMF-induced cell death was also observed in embryonic kidney cells (HEK293, HEK293T) exposed to an unmodulated 940 MHz carrier wave (SAR: 90 mW/kg) [ 218 ] or 2.45 GHz (E-field: 2 V/m) [ 219 , 220 ] for about 1 h. However, the results from the analyses of the markers for oxidative stress differed. While the 2.45 GHz RF-EMF resulted in higher MDA levels and reduced activities of SOD and GPx, exposure to the 940 MHz RF-EMF decreased MDA values over time while increasing SOD, with maximal changes observed at 30–45 min after the exposure started.…”

Section: Further Observations Of Oxidative Stress Induced By Emfmentioning

confidence: 99%

Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health

Schuermann

Mevissen

2021

IJMS

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Concomitant with the ever-expanding use of electrical appliances and mobile communication systems, public and occupational exposure to electromagnetic fields (EMF) in the extremely-low-frequency and radiofrequency range has become a widely debated environmental risk factor for health. Radiofrequency (RF) EMF and extremely-low-frequency (ELF) MF have been classified as possibly carcinogenic to humans (Group 2B) by the International Agency for Research on Cancer (IARC). The production of reactive oxygen species (ROS), potentially leading to cellular or systemic oxidative stress, was frequently found to be influenced by EMF exposure in animals and cells. In this review, we summarize key experimental findings on oxidative stress related to EMF exposure from animal and cell studies of the last decade. The observations are discussed in the context of molecular mechanisms and functionalities relevant to health such as neurological function, genome stability, immune response, and reproduction. Most animal and many cell studies showed increased oxidative stress caused by RF-EMF and ELF-MF. In order to estimate the risk for human health by manmade exposure, experimental studies in humans and epidemiological studies need to be considered as well.

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“…Önceki çalışmalarımızdan elde ettiğimiz sonuçlara göre, in vitro olarak çeşitli konsantrasyonlarda uygulanan sodyum selenitin normal hücre dizilerinde anti-oksidan olarak davrandığı belirlenmiştir. Sodyum selenitin 100-200 nM konsantrasyon aralığında, 48 saat süreyle uygulanmasının laktodehidragenaz (LDH) salınımını azalttığı, süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH) seviyelerini arttırdığı ve bu etkileri en fazla 200 nM konsantrasyonda gerçekleştirdiği gözlenmiştir [17,18]. Bu çalışmada selenyumun sodyum selenit formunda MCF-7 meme kanseri hücre dizisindeki etkisinin araştırılması hedeflenmiştir.…”

Section: Sonuçlar Ve Tartışmaunclassified

“…Daha önce yaptığımız çalışmalarda 48 saat süreyle sodyum selenitin çeşitli (100-200 nM) konsantrasyonlarının etkinliği araştırılmıştır. Bu çalışmaların sonunda normal hücre dizisinde reaktif oksijen türlerinin azalmasına destek olabileceği gösterilmiştir [17,18]. Araştırmamızda daha önceki çalışmalarımızda uygulanan ve etkin konsantrasyon olarak belirlenen 200 nM selenyumun meme kanseri (MCF-7) hücrelerinde hücre canlılığı, proliferasyonu, total antioksidan-oksidan kapasite ve oksidatif stres indeksi üzerine olan etkilerinin değerlendirilmesi amaçlanmıştır.…”

Section: Introductionunclassified

Meme kanseri hücre dizisinde (MCF-7) selenyumun rolü

Ergün¹,

Koç²,

Soyocak³

2021

Biological Diversity and Conservation

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Breast cancer is among the common causes of death in the world, it is known that genetic, endocrine and environmental factors play a role in its etiology. Intake of supplements in nutrition is important for the development of new agents for breast cancer treatment or to increase the effectiveness of existing drugs. It is suggested that micronutrients such as selenium taken from vegetable and animal foods such as seafood, legumes, meat, milk, and nuts may play a role in preventing or suppressing cancer by supporting the effectiveness of anticancer agents. In our study, 200 nM selenium was applied to a human breast cancer cell (MCF-7) line for 48 hours. Cell viability by trypan blue method, cell proliferation by XTT, total antioxidant (TAS)-oxidant capacity (TOS) and oxidative stress index (OSI) values were analyzed by ELISA method. A decrease was detected in cell viability, proliferation, TAS, TOS values in the selenium applied group compared to the control group not to statistically significant and an increase was observed in the OSI value. According to the results obtained in our study, it was determined that selenium concentration, which appears to be effective in normal cells, does not show the same effect on breast cancer cells.

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Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in the HEK293 cells

Cited by 15 publications

References 38 publications

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health

Meme kanseri hücre dizisinde (MCF-7) selenyumun rolü

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