Selenium nanoparticles: synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity

Boroumand, Safieh; Safari, Mojtaba; Shaabani, Elnaz; Shirzad, ‪Mahdieh; Faridi‐Majidi, Reza

doi:10.1088/2053-1591/ab2558

Cited by 126 publications

(76 citation statements)

References 26 publications

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“…SeNPs (reduced with ascorbic acid) produced a broad peak centred at 271 nm, indicating that the sodium selenite was chemically reduced into NPs corresponding to results seen in both chemical and green synthesis of SeNPs [ 15 , 21 , 25 ]. The PAMAM (G5) dendrimer in aqueous solution produced a weak peak at 283 nm, in agreement with reports that this particular peak appears when dendrimers are at physiological pH, and the tertiary amine groups are deprotonated [ 26 ]. PAMAM-SeNPs displayed a weak λ max peak at around 282 nm, indicating the presence of the dendrimers.…”

Section: Resultssupporting

confidence: 90%

Folate-Targeted Transgenic Activity of Dendrimer Functionalized Selenium Nanoparticles In Vitro

Pillay

Daniels

Singh

2020

IJMS

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Current chemotherapeutic drugs, although effective, lack cell-specific targeting, instigate adverse side effects in healthy tissue, exhibit unfavourable bio-circulation and can generate drug-resistant cancers. The synergistic use of nanotechnology and gene therapy, using nanoparticles (NPs) for therapeutic gene delivery to cancer cells is hereby proposed. This includes the benefit of cell-specific targeting and exploitation of receptors overexpressed in specific cancer types. The aim of this study was to formulate dendrimer-functionalized selenium nanoparticles (PAMAM-SeNPs) containing the targeting moiety, folic acid (FA), for delivery of pCMV-Luc-DNA (pDNA) in vitro. These NPs and their gene-loaded nanocomplexes were physicochemically and morphologically characterized. Nucleic acid-binding, compaction and pDNA protection were assessed, followed by cell-based in vitro cytotoxicity, transgene expression and apoptotic assays. Nanocomplexes possessed favourable sizes (<150 nm) and ζ-potentials (>25 mV), crucial for cellular interaction, and protected the pDNA from degradation in an in vivo simulation. PAMAM-SeNP nanocomplexes exhibited higher cell viability (>85%) compared to selenium-free nanocomplexes (approximately 75%), confirming the important role of selenium in these nanocomplexes. FA-conjugated PAMAM-SeNPs displayed higher overall transgene expression (HeLa cells) compared to their non-targeting counterparts, suggesting enhanced receptor-mediated cellular uptake. Overall, our results bode well for the use of these nano-delivery vehicles in future in vivo studies.

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Section: Resultssupporting

confidence: 90%

Folate-Targeted Transgenic Activity of Dendrimer Functionalized Selenium Nanoparticles In Vitro

Pillay

Daniels

Singh

2020

IJMS

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“…Dispersions were found to be stable against aggregation [54]. The previous study state that the SeNPs showed antibacterial activity against P. aeruginosa (9.25 mm) and S. aureus (10.75 mm) [55]. In the present study, the synthesized SeNPs stabilized with EWL exhibited stronger antibacterial effect against pathogenic bacteria.…”

Section: Discussionsupporting

confidence: 57%

A rapid synthesis and antibacterial property of selenium nanoparticles using egg white lysozyme as a stabilizing agent

et al. 2019

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A simple method for the rapid synthesis of selenium nanoparticles (SeNPs) by the reduction of selenium dioxide using an aqueous egg white lysozyme solution (stabilizing agent) and ascorbic acid solution (reducing agent) has been described. The formation of SeNPs is assured by characterization with UV-Vis, FT-IR, XRD and morphological characters that were observed using TEM analyses. The absorbance of the SeNPs is found at 250 nm. The TEM images show that the SeNPs are uniform and in spherical shape of size 40-60 nm. The crystalline nature of SeNPs is assured by XRD analysis. The antibacterial property of synthesized SeNPs was tested against the pathogenic bacteria, i.e., Bacillus subtilis, Bacillus cereus, Escherichia coli, Staphylococcus pneumoniae, Proteus mirabilis and Klebsiella pneumoniae. SeNPs showed more antibacterial activity against B. subtilis (19 mm) and Streptococcus pneumoniae (15 mm) as compared with commercially available antibiotics such as streptomycin and erythromycin. The SeNPs synthesized via this process has proficient antimicrobial activity against pathogenic bacteria. SeNPs could play a significant role in pharmaceutical industries for the development of efficient antibiotic agents.

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“…(κ16)-coated Se NPs is very high compared to previously reported PVA coated Se NPs, which normally show a minimum inhibitory concentration (MIC) higher than 60 µg/mL 9,17,34,35 against S. aureus, and even worse performance against E. coli, with no significant effect, 9,24,25,35 or MIC values higher than 100 µg/mL. 17,34,94 Although the MBC was not tested in most of these studies, the MBC is generally higher than the MIC.…”

Section: Cytotoxicity Test Of Selenium Nanoparticles Using Fibroblastmentioning

confidence: 88%

“…In fact, the chitosan coated Se NPs were found to show much higher MIC than the PVA coated Se NPs against both S. aureus (500 µg/mL vs 125 µg/mL) and E. coli (500 µg/mL vs 250 µg/mL). 35 This unexpected result may be due to different sized NPs being used with the different coatings (195 nm for the chitosan coated Se NPs vs 136 nm for the PVA coated Se NPs). Importantly, size is one key factor influencing the uptake of NPs by cells in general and the antibacterial efficacy of Se NPs in particular.…”

Section: Introductionmentioning

confidence: 99%

“…PVA is a commonly used stabilizing agent for Se NPs, 57,58 and the antibacterial activity of PVA coated Se NPs have been investigated in many studies. 9,23,35,59 Furthermore, the eADF4(κ16)coated NPs were immobilised on positively or negatively charged spider silk protein films, and their ability to exert their antibacterial activity was assessed.…”

Section: Introductionmentioning

confidence: 99%

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<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Huang¹,

Kumari²,

Herold³

et al. 2020

IJN

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Purpose: Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Methods: Se NPs were coated with a positively charged proteinrecombinant spider silk protein eADF4(κ16)to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4(κ16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Results: Compared to the negatively charged polyvinyl alcohol coated Se NPs, the positively charged eADF4(κ16)-coated Se NPs demonstrated a much higher bactericidal efficacy against the Gram-negative bacteria E. coli, with a minimum bactericidal concentration (MBC) approximately 50 times lower than that of negatively charged Se NPs. Cytotoxicity testing showed that the eADF4(κ16)-coated Se NPs are safe to both Balb/3T3 mouse embryo fibroblasts and HaCaT human skin keratinocytes up to 31 µg/mL, which is much higher than the MBC of these particles against E. coli (8 ± 1 µg/mL). In addition, antibacterial coatings were created by immobilising the eADF4(κ16)-coated Se NPs on positively charged spider silk films and these were shown to retain good bactericidal efficacy and overcome the issue of low particle stability in culture broth. It was found that these Se NPs needed to be released from the film surface in order to exert their antibacterial effects and this release can be regulated by the surface charge of the film, such as the change of the spider silk protein used. Conclusion: Overall, eADF4(κ16)-coated Se NPs are promising new antibacterial agents against life-threatening bacteria.

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Selenium nanoparticles: synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity

Cited by 126 publications

References 26 publications

Folate-Targeted Transgenic Activity of Dendrimer Functionalized Selenium Nanoparticles In Vitro

Folate-Targeted Transgenic Activity of Dendrimer Functionalized Selenium Nanoparticles In Vitro

A rapid synthesis and antibacterial property of selenium nanoparticles using egg white lysozyme as a stabilizing agent

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

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