Selenium Nanoparticles by Moderating Oxidative Stress Promote Differentiation of Mesenchymal Stem Cells to Osteoblasts

Fatima, Sabiha; Alfrayh, Rawan; Alrashed, May; Alsobaie, Sarah; Ahmad, Rehan; Mahmood, Amer

doi:10.2147/ijn.s285233

Cited by 33 publications

(24 citation statements)

References 40 publications

(46 reference statements)

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“…Fatima et al . [ 46 ] confirmed that low concentrations of SeNPs enhanced the cell viability and osteogenic potential of human mesenchymal stem cells and increased c-Jun N-terminal kinase (JNK) and Forkhead box O3 (FOXO3) expression, suggesting that SeNPs might enhance osteogenesis via activation of the JNK/FOXO3 pathway. In addition, the protective effects of Se on bone health may involve other selenoproteins that affect the osteogenic potential of BMMSCs.…”

Section: Discussionmentioning

confidence: 96%

Selenium-modified bone cement promotes osteoporotic bone defect repair in ovariectomized rats by restoring GPx1-mediated mitochondrial antioxidant functions

Zhou

Chen

et al. 2023

Regenerative Biomaterials

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Over-accumulation of reactive oxygen species (ROS) causes mitochondrial dysfunction and impairs the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMMSCs). Selenium (Se) protects BMMSCs from oxidative stress-induced damage; however, it is unknown whether Se supplementation can promote the repair of osteoporotic bone defects by rescuing the impaired osteogenic potential of osteoporotic BMMSCs (OP-BMMSCs). In vitro treatment with sodium selenite (Na2SeO3) successfully improved the osteogenic differentiation of OP-BMMSCs, as demonstrated by increased matrix mineralization and up-regulated osteogenic genes expression. More importantly, Na2SeO3 restored the impaired mitochondrial functions of OP-BMMSCs, significantly up-regulated glutathione peroxidase 1 (GPx1) expression, and attenuated the intracellular ROS and mitochondrial superoxide. Silencing of Gpx1 completely abrogated the protective effects of Na2SeO3 on mitochondrial functions of OP-BMMSCs, suggesting the important role of GPx1 in protecting OP-BMMSCs from oxidative stress. We further fabricated Se-modified bone cement based on silk fibroin and calcium phosphate cement (SF/CPC). After eight weeks of implantation, Se-modified bone cement significantly promoted bone defect repair, evidenced by the increased new bone tissue formation and enhanced GPx1 expression in ovariectomized rats. These findings revealed that Se supplementation rescued mitochondrial functions of OP-BMMSCs through activation of the GPx1-mediated antioxidant pathway, and more importantly, supplementation with Se in SF/CPC accelerated bone regeneration in ovariectomized rats, representing a novel strategy for treating osteoporotic bone fractures or defects.

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Section: Discussionmentioning

confidence: 96%

Selenium-modified bone cement promotes osteoporotic bone defect repair in ovariectomized rats by restoring GPx1-mediated mitochondrial antioxidant functions

Zhou

Chen

et al. 2023

Regenerative Biomaterials

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“…In recent years, the coupling of bone formation and bone resorption by osteoblasts and osteoclasts has still been the focus of the treatment of osteoporosis [ 61 ]. The effect of regulating oxidative stress and autophagy on osteoporosis has also aroused widespread concern [ 62 ]. As a classic and particularly important pathway in regulating osteogenic differentiation of BMSCs, the BMP-SMAD signal has become a new hot spot and breakthrough in the treatment of osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Luo

Xia

Wang³

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. The Jiawei Yanghe decoction (JWYHD) is a traditional Chinese medicine formula for the treatment of osteoporosis, but its therapeutic mechanism has not been fully elucidated, and the therapeutic target of the intervention disease needs to be further verified. The dysfunction of bone mesenchymal stem cells (BMSCs) is considered to be an important pathogenesis of postmenopausal osteoporosis (PMOP). The purpose of this study was to explore how JWYHD regulates BMSC differentiation through the BMP-SMAD signal pathway. Methods. In the in vivo study, we used an ovariectomized PMOP rat (n = 36, 2-month-old, 200 ± 20 g) model and femur micro-CT analysis to study the effect of JWYHD on bone loss in rats. By immunofluorescence, the translocation expression of BMP2, a key protein in the pathway, was detected. Serum bone metabolism was detected by an enzyme-linked immunosorbent assay (ELISA). Alkaline phosphatase (ALP) activity was detected by alkaline phosphatase staining (ALPS), osteogenesis and matrix mineralization were detected by alizarin red staining (ARS), the adipogenic ability of BMSCs was detected by oil red staining (ORS), and CFU is used to detect the ability of cells to form colonies. The expression of related proteins was detected by western blotting. Results. In vivo and in vitro, the OP phenotypes of SD rats induced by ovariectomy (OVX) included impaired bone mineral density and microstructure, abnormal bone metabolism, and impaired MSC differentiation potential. JWYHD treatment reversed this trend and restored the differentiation potential of MSCs. JWYHD medicated serum and direct intervention of drugs activated the BMP-SMAD signaling pathway, promoted the osteogenic differentiation of BMSCs, and inhibited their adipogenic differentiation. Conclusions. Our data identified that JWYHD is an effective alternative drug for the treatment of PMOP that functions to stimulate the differentiation of BMSCs into osteoblasts in the BMP-SMAD signaling-dependent mechanism.

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“…The combination of galantamine nanoparticles and MSCs resulted in signi cant protective effects against isoproterenol-induced amnesia (Misra et al, 2016). SeNPs increased cell viability and differentiation of human embryonic stem cells to an osteogenic cell in vitro and facilitated neural differentiation in MSCs (Fatima et al, 2021)…”

Section: Discussionmentioning

confidence: 99%

Effect of co-treatment with mesenchymal stem cells and polyvinyl alcohol- coated selenium nanoparticles on rats with streptozotocin-induced Alzheimer's disease

Shahidi

Asl

Gholamigeravand

et al. 2022

Preprint

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Mesenchymal stem cell (MSC) transplantation is a potential strategy for the treatment of Alzheimer’s disease (AD). The stem cells have a short lifespan after transplantation in the recipient animals. Selenium nanoparticles facilitate drug treatment in brain diseases due to their size. The aim of this study was to investigate the possible therapeutic effect of co-treatment of MSCs with polyvinyl alcohol (PVA)-coated selenium nanoparticles (SeNPs) against streptozotocin (STZ)-mediated AD in rats. Rats were assigned to five groups: control, Alz (STZ; 3 mg/kg, 10 μL, ICV), Alz+stem cell (ICV transplantation), Alz+SeNP (0.4 mg/kg, orally), and Alz+stem cell+SeNPs. ICV administration of STZ mimicked some aspects of AD in Alz groups. SeNPs were administrated for 30 days after STZ administration. The novel object recognition (NOR) and passive avoidance learning (PAL) tests were used to measure cognition and memory. Oxidative stress biomarkers, brain-derived neurotrophic factor (BDNF), and amyloid-beta plaques were assessed by biochemical analysis, ELISA kit, and Congo red staining, respectively. The combined treatment of PVA-coated SeNPs and MSCs transplantation was more effective in increasing the memory reacquisition than the administration of SeNPs or MSCs alone. The stem cells and PVA-coated SeNPs together significantly increased the antioxidant capacity and reduced the amyloid-beta plaques. It seems that co-treatment with PVA-coated SeNP with MSCs has a great neuroprotection effect against AD in animal models.

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Selenium Nanoparticles by Moderating Oxidative Stress Promote Differentiation of Mesenchymal Stem Cells to Osteoblasts

Cited by 33 publications

References 40 publications

Selenium-modified bone cement promotes osteoporotic bone defect repair in ovariectomized rats by restoring GPx1-mediated mitochondrial antioxidant functions

Selenium-modified bone cement promotes osteoporotic bone defect repair in ovariectomized rats by restoring GPx1-mediated mitochondrial antioxidant functions

Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Effect of co-treatment with mesenchymal stem cells and polyvinyl alcohol- coated selenium nanoparticles on rats with streptozotocin-induced Alzheimer's disease

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