Selenium Nanoparticles Attenuate Oxidative Stress and Testicular Damage in Streptozotocin-Induced Diabetic Rats

Abstract

Abstract:We investigated the protective and antioxidative effects of selenium nanoparticles (SeNPs) in streptozotocin STZ-induced diabetic rats. STZ-diabetic rats were exposed daily to treatments with SeNPs and/or insulin and then the effect of these treatments on the parameters correlated to oxidative damage of the rat testes were assessed. Biochemical analysis revealed that SeNPs are able to ameliorate the reduction in the serum testosterone caused by STZ-induced diabetes. Furthermore, SeNPs could significantly decrease testicular tissue oxidative stress markers, namely lipid peroxidation and nitric oxide. In contrast, treatment of the STZ-diabetic rats with SeNPs increased the glutathione content and antioxidant enzyme activities in testicular tissues. Moreover, microscopic analysis proved that SeNPs are able to prevent histological damage in the testes of STZ-diabetic rats. Molecular analysis revealed that the mRNA level of Bcl-2 (B-cell lymphoma 2) is significantly upregulated. On the contrary, the mRNA level of Bax (Bcl-2 Associated X Protein) was significantly downregulated. Furthermore, treatment of STZ-diabetic rats with SeNPs led to an elevation in the expression of PCNA (Proliferating Cell Nuclear Antigen Gene). Interestingly, the insulin treatment also exhibited a significant improvement in the testicular function in STZ-diabetic rats. Collectively, our results demonstrated the possible effects of SeNPs in attenuating diabetes-induced oxidative damage, in particular in testicular tissue.

Vitamin E improves testicular damage in streptozocin-induced diabetic rats, via increasing vascular endothelial growth factor and poly(ADP-ribose) polymerase-1

“…In our study, we showed that diabetes was associated with an oxidative stress state evidenced by a significantly increased testicular MDA level (lipid peroxidation) in the untreated diabetic rats. Ramzy, El‐Sheikh, Kamel, Abdelwahab, and Morsy () and Dkhil, Zrieq, Al‐Quraishy, and Abdel Moneim () also showed that the diabetic process was associated with a significant increase in testicular tissue MDA levels.…”

“…Dkhil et al. () found that induction of diabetes in rats resulted in reduction of Sertoli and spermatogenic cells and was associated with degeneration in the spermatogenic cell series and thickened interstitial vessel walls. Diabetes has also been reported to reduce the number of spermatogenic cells and the diameter of the seminiferous tubules by Guneli et al.…”

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

“…Reduction in E. coli and P. aeruginosa cells was also perceived after 24 h. A decrease of 2.46, 2.95 7). It was evident that the maximum killing of E. coli, P. aeruginosa and S. aureus cells with nSe was observed after 12 h. Thus, it can be strongly interpreted that the nSe effectively inhibited the growth of Gram positive and Gram negative bacterial strains in a time dependent manner, thereby highlighting its bacteriostatic effect [59,60].…”

Trace Elements, PPARs, and Metabolic Syndrome

“…Trace elements, such as iron, zinc, copper, chromium, selenium, and so on, are micronutrients involved in hundreds of biological processes, including inflammation [26], oxidative stress [27], and lipid metabolism [28]. These elements account for a low percentage of total body weight (less than 0.01%) [29].…”

“…In contrast, insufficient silicon intake is associated with a high risk of cardiovascular events and increases the case fatality rate [84]. Selenium often acts as a redox scavenger in the human body [27], an action which is essential to the restoration of impaired islets and vascular tissues in diabetes [85,86]. The liver is one of the organs with the highest selenium concentration [87].…”