Selenium modulates MMP2 expression through the TGFβ1/Smad signalling pathway in human umbilical vein endothelial cells and rabbits following lipid disturbance

Xu, Chenggui; Liu, Guihua; Li, Qinglang; Zhang, Juhong; Huang, Zhibin; Gao, Xiuren

doi:10.1016/j.jtemb.2017.04.006

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…TGF-β1-dependent pathway could promote the elevation of endothelial VCAM-1 (Sucosky, Balachandran, Elhammali, Jo, & Yoganathan, 2009). Moreover, elevated expressions of TGF-β1, pSmad2, pSmad3, and Smad3 could further enhance the expression of MMP-2 (Xu et al, 2017).…”

Section: Discussionmentioning

confidence: 98%

“…TGF‐β1‐dependent pathway could promote the elevation of endothelial VCAM‐1 (Sucosky, Balachandran, Elhammali, Jo, & Yoganathan, ). Moreover, elevated expressions of TGF‐β1, pSmad2, pSmad3, and Smad3 could further enhance the expression of MMP‐2 (Xu et al, ). Similarly, it has been proved that the activation of Smad1/5 via TGF‐β1 could trigger HAMP and PLAUR, which could result in atherosclerotic plaque vulnerability (Nurgazieva et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Retracted: Downregulated microRNA‐224 aggravates vulnerable atherosclerotic plaques and vascular remodeling in acute coronary syndrome through activation of the TGF‐β/Smad pathway

Sui

Sun

et al. 2018

Journal Cellular Physiology

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Recent studies have shown that circulating microRNAs (miRNA) play a critical role in diagnosing acute coronary syndrome (ACS). This study aims to investigate the effect of miR‐224 on atherosclerotic plaques forming and vascular remodeling in ACS and its relationship with TGF‐β/Smad pathway. Myocardial infarction (MI) rat model was established and lentivirus vector of miR‐224 inhibitor was prepared for investigating the effect of downregulated miR‐224 on the contents of nitric oxide (NO) and endothelin‐1 (ET‐1), blood lipid levels and inflammatory factor levels in serum as well as the TGF‐β/Smad pathway. The rats suffering from MI had decreased survival rates and exhibited reduced levels of NO, high‐density lipoprotein cholesterol, and lumen diameter, and Smad7 messenger RNA (mRNA) and protein expression; while had significantly increased ratio of heart weight or body weight, levels of ET‐1, inflammatory factors, blood lipid indexes, vascular remodeling indexes, collagen volume fraction, vulnerable atherosclerotic plaque area, VCAM‐1 and MMP‐2 protein expression, TGF‐β, Smad2, Smad3, and Smad4 mRNA and protein expression. After inhibiting the TGF‐β/Smad pathway, the rats suffering from MI showed notably opposite trend. In conclusion, downregulation of miR‐224 expression promotes the formation of vulnerable atherosclerotic plaques and vascular remodeling in ACS through activation of the TGF‐β/Smad pathway. Therefore, this study provides a new therapeutic target for ACS.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Retracted: Downregulated microRNA‐224 aggravates vulnerable atherosclerotic plaques and vascular remodeling in acute coronary syndrome through activation of the TGF‐β/Smad pathway

Sui

Sun

et al. 2018

Journal Cellular Physiology

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“…TMEPA1 may be a downstream effector of Smad and regulator of PTEN; in Smad3-knockdown TNBC cancer cells, decreased growth, motility, and invasion were reversed by overexpressing TMEPA1. Upregulated TMEPA1 has been shown to decrease PTEN, resulting in increased TGF-β–dependent tumor growth, motility, invasion, and cancer progression in TNBC [ 37 ]. Thus, the TGF-β-Smad2/Smad3-TMEPA1-PTEN axis may play a pivotal role in targeted therapy for TNBC.…”

Section: Discussionmentioning

confidence: 99%

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice

et al. 2021

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Fish oil (FO) and selenium (Se) possess antiangiogenic potential in malignant tumors. This study aimed to determine whether combination of FO and Se enhanced treatment efficacy of low-dose antiangiogenic agent Avastin (bevacizumab) in a dose-dependent manner and targeted multiple signaling pathways in triple-negative breast cancer (TNBC)-bearing mice. Randomized into five groups, mice received treatment with either physiological saline (control), Avastin alone, or Avastin in combination with low, medium, and high doses of FO/Se. The target signaling molecules for anticancer were determined either by measuring protein or mRNA expression. Avastin-treated mice receiving FO/Se showed lower tumor growth and metastasis than did mice treated with Avastin alone. Combination-treated mice exhibited lower expressions in multiple proangiogenic (growth) factors and their membrane receptors, and altered cytoplasmic signaling molecules (PI3K-PTEN-AKT-TSC-mTOR-p70S6K-4EBP1, Ras-Raf-MEK-ERK, c-Src-JAK2-STAT3-TMEPAI-Smad, LKB1-AMPK, and GSK3β/β-catenin). Dose-dependent inhibition of down-stream targets including epithelial-to-mesenchymal transition transcription factors, nuclear cyclin and cyclin-dependent kinases, cancer stem cell markers, heat shock protein (HSP-90), hypoxia-inducible factors (HIF-1α/-2α), matrix metalloprotease (MMP-9), and increased apoptosis were observed. These results suggest that combination treatment with FO and Se increases the therapeutic efficacy of Avastin against TNBC in a dose-dependent manner through multiple signaling pathways in membrane, cytoplasmic, and nucleic targets.

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“…The catechins found in green tea have been shown to effectively inhibit MMP-2 activities in the glioblastomas cells [32]. Furthermore, there are some studies which have demonstrated that trace elements modulated MMPs activity and improved cell migration and invasion in different kinds of disease [33-35]. In this study, cold stress in broiler chickens induced ascites which was associated with increased MMP-2 activity in liver and brain, while copper-methionine supplementation alleviated the problem.…”

Section: Discussionmentioning

confidence: 99%

Oral copper-methionine decreases matrix metalloproteinase-2 activity in the liver and brain of broiler chickens subjected to cold stress for ascites incidence

Varzaneh,

Rahmani,

Jahanian

et al. 2023

Preprint

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Copper plays an antioxidant role in biological reactions. This study examined the impact of copper-methionine supplementation on the matrix metalloproteinase-2 (MMP-2) activity and gene expression in the liver and brain of broiler chickens subjected to cold temperature. A total of 480 broiler chickens were assigned to 6 groups and reared under either low (15-19 C) or normal temperature (25-28 C) and fed a basal diet enriched with different concentrations of copper-methionine (Cu-Met) supplementation (0, 100 or 200 mg.kg-1). Ascites was exclusively observed in broiler chickens kept in low temperature and fed with basal diet without Cu-Met during the seventh week, identified by the presence of abdominal fluid accumulation. Broilers' livers and brains were separated for MMP-2 and tissue inhibitor of metalloproteinase-2 (TIMP-2) analysis. Results of gelatin zymography on these samples demonstrated that incidence of ascites was associated with increased MMP-2 levels in liver and brain. MMP-2 activity assay confirmed the results obtained by zymography. RT-qPCR experiments revealed an upregulation in the mRNA expression of MMP-2. In contrast, the treatments did not induce significant alterations in TIMP-2 levels. Results suggest that oral copper-methionine can decrease the ascites occurrence and might be useful for prevention of ascites in broiler chickens. Key words: Matrix metalloproteinase-2 (MMP-2); Tissue inhibitor of metalloproteinase-2 (TIMP-2); Copper-methionine; Liver; Brain; Broiler chicken; Ascites; Cold stress

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Selenium modulates MMP2 expression through the TGFβ1/Smad signalling pathway in human umbilical vein endothelial cells and rabbits following lipid disturbance

Cited by 10 publications

References 41 publications

Retracted: Downregulated microRNA‐224 aggravates vulnerable atherosclerotic plaques and vascular remodeling in acute coronary syndrome through activation of the TGF‐β/Smad pathway

Retracted: Downregulated microRNA‐224 aggravates vulnerable atherosclerotic plaques and vascular remodeling in acute coronary syndrome through activation of the TGF‐β/Smad pathway

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice

Oral copper-methionine decreases matrix metalloproteinase-2 activity in the liver and brain of broiler chickens subjected to cold stress for ascites incidence

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