Selenium fractionation from plasma, muscle and liver of Nile tilapia (Oreochromis niloticus)

Silva, Fábio A.; Cavecci, Bruna; Baldassini, Welder Angelo; Lima, Paula Monteiro de; Moraes, Paula Martin de; Roldan, Paulo S.; Padilha, Cilene C. F.; Padilha, Pedro M.

doi:10.1007/s11694-013-9151-6

Cited by 9 publications

(12 citation statements)

References 21 publications

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“…For 2D-PAGE separation, only the pellets from the second precipitation (Mw \ 90 kDa) were used. The two electrophoretic steps were performed as described previously (Lima et al 2010;Santos et al 2011;Neves et al 2012;Silva et al 2013).…”

Section: Electrophoretic Runs (2d-page)mentioning

confidence: 99%

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Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunaré (Cichla spp.) from the Amazon Region of Brazil

Vieira

Cavecci

Queiroz

et al. 2015

Arch Environ Contam Toxicol

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This study used metalloproteomic techniques to characterize mercury (Hg)-bound proteins in the muscle and liver tissue of Tucunaré (Cichla spp.) collected at the Jirau Hydroelectric Power Plant in Madeira River Basin, Brazil. The proteome of the muscle and liver tissue was obtained after two steps of fractional precipitation and separating the proteins by 2-D polyacrylamide gel electrophoresis. Hg was identified and quantified in the protein spots by graphite furnace atomic absorption spectrometry after acid mineralization in an ultrasound bath. Hg with a molecular weight <20 kDa and a concentration between 13.30 and 33.40 mg g(-1) was found in the protein spots. These protein spots were characterized by electrospray ionization tandem mass spectrometry after trypsin digestion. From a total of 12 analyzed spots, seven proteins showing Hg biomarker characteristics were identified: parvalbumin and its isoforms, ubiquitin-40S ribosomal protein S27a, zinc (Zn) finger and BTB domain-containing protein 24, and dual-specificity protein phosphatase 22-B.

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Section: Electrophoretic Runs (2d-page)mentioning

confidence: 99%

“…After stain removal, the gels were imaged with a scanner (GE Healthcare, Uppsala, Sweden) and analyzed using ImageMaster 2D Platinum 7.0 software (GE Healthcare 2007) as described previously (Lima et al 2010;Santos et al 2011;Neves et al 2012;Moraes et al 2012;Silva et al 2013;Braga et al 2015).…”

Section: Electrophoretic Runs (2d-page)mentioning

confidence: 99%

Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunaré (Cichla spp.) from the Amazon Region of Brazil

Vieira

Cavecci

Queiroz

et al. 2015

Arch Environ Contam Toxicol

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“…The fish species Brachyplatystoma filamentosum is also the most consumed by the riverside population of the Madeira River. Thus, consumption of Brachyplatystoma filamentosum may pose a risk to the health of these coastal communities from the Brazilian Amazon [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

Queiroz

Vieira

Oliveira

et al. 2018

Biol Trace Elem Res

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Predator fish can accumulate high levels of mercury, which qualifies them as potential indicators of this toxic metal. The predatory species Brachyplatystoma filamentosum, popularly known as filhote, is among the most consumed species in the Brazilian Amazon. Continuing the metalloproteomic studies of mercury in Amazonian fishes that have been developed in the last 5 years, the present paper provides the data of protein characterization associated with mercury in muscle and liver samples of filhote (Brachyplatystoma filamentosum) collected in the Madeira River, Brazilian Amazon. The mercury concentration in the muscle and liver samples was determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein fraction was extracted in an aqueous medium, and later, a fractional precipitation procedure was performed to obtain the protein pellets. Then, the proteome of the tissue samples of this fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and a mercury mapping of the protein spots was carried out by GFAAS after acid digestion. Protein spots that had mercury were characterized by mass spectrometry with electrospray ionization in sequence (ESI-MS/MS) after tryptic digestion. It was possible to characterize 11 mercury-associated protein spots that presented biomarker characteristics and could be used to monitor mercury in fish species of the Amazon region. Thus, the metalloproteomic strategies used in the present study allowed us to characterize 11 mercury-associated protein spots. It should be noted that the protein spots identified as GFRP, TMEM186, TMEM57B, and BHMT, which have coordination sites for elements with characteristics of soft acids, such as mercury, can be used as biomarkers of mercury contamination in monitoring studies of this toxic metal in fish species from the Amazon region.

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“…O clima na bacia do rio Solimões, é bem semelhante ao da bacia amazônica, sendo quente e úmido, com precipitação pluvial anual igual ou superior a 2.000 mm e com médias anuais de temperatura e umidade relativa de 27 °C e 84%, respectivamente (GUIMARÃES et al, 2013). Essas fitas foram colocadas na caixa de hidratação IPGBOX, cobertas com óleo mineral e mantidas à temperatura ambiente por aproximadamente 12 h. Após esse período, as fitas foram transferidas para o isoeletrofocalizador EttanTM IPGphorTM 3 para realização da primeira dimensão do processo eletroforético (LIMA et al, 2010;NEVES et al, 2012;SANTOS et al, 2011;SILVA et al, 2013).…”

Section: Rio Apuaúunclassified

“…O corante ficou em contato com o gel durante 72 h em mesa agitadora a 40 rpm e depois foi removido através de sucessivas lavagens com água ultrapura até que as proteínas (spots) ficassem visíveis (LIMA et al, 2010;NEVES et al, 2012;SANTOS et al, 2011;SILVA et al, 2013 Já a estatística descritiva foi utilizada para apresentar características gerais (comprimento, peso e concentração de mercúrio total) dos tucunarés por local de amostragem.…”

Section: Rio Purusunclassified

Estratégias metaloproteômicas na investigação de biomarcadores de mercúrio em amostras de tecido muscular de tucunarés

Figueiredo¹

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Formação acadêmica -2012Graduação em Gestão Ambiental. Universidade de Brasília, UnB, Brasil total no tecido muscular de tucunarés capturados em rios e reservatórios hidrelétricos.As concentrações apresentaram pouca variação independente do local de amostragem e algumas estavam superiores aos limites máximos definidos na legislação brasileira. As concentrações aumentaram na sequência: reservatório de Cana Brava < reservatório de Balbina < rio Madeira < rio Purus < reservatório de Tucuruí < rio Apuaú < rio Solimões < rio Negro. As amostras do rio Negro foram selecionadas para definição de um biomarcador proteico através da eletroforese bidimensional (2D-PAGE). O mercúrio foi determinado em 15 possíveis proteínas com massa molar abaixo de 20,1 kDa, sendo 2 mais promissoras à biomarcadoras. Além disso, foi extraído da musculatura dos tucunarés apenas 12,4% do mercúrio, sendo que 89% desse mercúrio foi aplicado na técnica 2D-PAGE, resultando em 49,74% de mercúrio nas proteínas. Essa porcentagem de mercúrio nos spots indica que os procedimentos otimizados de preparação da amostra foram eficientes para ser aplicados na 2D-PAGE. Os resultados metaloproteômicos desse estudo são importantes no subsídio de estudos futuros de identificação e caracterização de biomarcadores de mercúrio em peixes.Descritores: biomarcadores; metaloproteínas; contaminantes; peixes. Thus, this study sought to identify possible mercury exposure biomarkers in tucunares the Negro river through metalproteomics methods. Initially, were determined the total mercury concentrations in muscle tissue of tucunares caught in rivers and hydroelectric reservoirs. They were in a little independent variation of the sampling site and some were above the maximum limits set by Brazilian law. The concentrations increased following: Cana Brava reservoir < Balbina reservoir < Madeira river < Purus river < Tucurui reservoir < Apuau river < Solimões river < Negro river. Samples of the Negro river were selected for definition of a protein biomarker by two-dimensional electrophoresis (2D-PAGE). Mercury was determined in 15 possible protein with molecular weight of 20.1 kDa below, 2 the most promising biomarkers. Moreover, it was extracted from the muscle of tucunarés only 12.4% of mercury, with 89% of mercury was applied to the 2D-PAGE technique, and 49.74% of mercury in proteins.This percentage of mercury in spots indicates that the sample preparation procedures were optimized for efficient be applied to 2D-PAGE. The metalproteomics results of this study are important in the allowance for futures studies of identification and characterization of mercury biomarkers in fish.

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Selenium fractionation from plasma, muscle and liver of Nile tilapia (Oreochromis niloticus)

Cited by 9 publications

References 21 publications

Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunaré (Cichla spp.) from the Amazon Region of Brazil

Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunaré (Cichla spp.) from the Amazon Region of Brazil

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

Estratégias metaloproteômicas na investigação de biomarcadores de mercúrio em amostras de tecido muscular de tucunarés

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