Selenium downregulates auxin and ethylene biosynthesis in rice seedlings to modify primary metabolism and root architecture

Malheiros, Rafael Soares Pozzi; Costa, Lucas Cavalcante da; Ávila, Rodrigo T.; Pimenta, Thaline M.; Teixeira, Lubia S.; Brito, Fred A.L.; Zsögön, Agustín; Araújo, Wagner L.; Ribeiro, Dimas M.

doi:10.1007/s00425-019-03175-6

Cited by 49 publications

(13 citation statements)

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“…Finally, we observed a significant increase in seminal root length upon treatment with the ethylene perception inhibitor AgNO 3 , also corresponding to previous findings [91]. An ACC treatment had been found to significantly increase the shoot and decrease the root [92], but in our experiment, the length of total shoot had significantly increased, but that of the internode and the root did not differ significantly.…”

Section: Methods Validationsupporting

confidence: 91%

Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

et al. 2020

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Background Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis. Results The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice. Conclusions The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general.

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Section: Methods Validationsupporting

confidence: 91%

Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

et al. 2020

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“…Malheiros et al (2019) observed that Se can interfere with auxin and ethylene balance and also is negatively related to the expression of genes associated with auxin transport. Since auxins are responsible not only for rooting but also for cell enlargement of all plant organs (Tian et al 2018, Toungos 2018, Ma and Li 2019, a decrease in size and mass of A. imperialis may be a hormonal response instead of a reflection of stress.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic apparatus performance and anatomical modulations of Alcantarea imperialis (Bromeliaceae) exposed to selenium during in vitro growth

Martins¹,

Moreira²,

Braga³

et al. 2021

Photosynt.

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Ft -fluorescence at time t after the beginning of actinic illumination; FV/F0 -ratio of the de-excitation rate constants for photochemical and nonphotochemical events; OEC -oxygen-evolving complex; PItotal -total performance index, which measures the performance up until the final electron acceptors of PSI; RC/CSm -total number of PSII active reaction centers; Sm/tFmax -average fraction of open RC in the period of 0 to tFmax (time of maximum fluorescence production); VI -relative variable fluorescence at 30 ms (step I); VJ -relative variable fluorescence at 2 ms (step J); VK -relative variable fluorescence at 0.3 ms (step K); WK -the damage to OEC; WL -indicates disturbance in the thylakoid membranes, reducing the energetic connectivity between the PSII units; δR0 -efficiency/probability with which an electron from the intersystem electron carriers moves to reduce end electron acceptors at the PSI acceptor side (RE); φD0quantum yield of energy dissipation (at t = 0); φE0 -quantum yield of electron transport (at t = 0); φP0 = FV/Fm -maximum quantum yield of primary photochemistry of PSII reaction center (at t = 0); φR0 -quantum yield of reduction of end electron acceptors at the PSI acceptor side (RE); ψR0 -efficiency/probability by which electrons move from PSII to the PSI acceptor side.

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“…Recently, a direct interaction between Se and ethylene was elegantly demonstrated in experiments involving cadmium stress alleviation in wheat (Iqbal et al, 2015), tomato fruit ripening (Zhu et al, 2017), and control of primary root growth in the rice system (Malheiros et al, 2019). These independent but complementary studies generated shreds of evidence that such responses were a consequence of an ethylene biosynthesis downregulation induced by Se.…”

Section: Model For Se-induced Downregulation Of Ethylene Biosynthesismentioning

confidence: 99%

“…Recently, several studies have uncovered evidence of a relationship between Se and ethylene metabolism in plants (Malorgio et al, 2009;Iqbal et al, 2015;Zhu et al, 2017;Hajiboland et al, 2019;Malheiros et al, 2019). In this vein, Malheiros et al (2019) demonstrated that Se partially inhibits ethylene biosynthesis in roots of rice seedlings. Likewise, Iqbal et al (2015) evidenced that Se inhibits 1-aminocyclopropane-1-carboxylate synthase (ACS) activity in wheat, the main limiting step of ethylene production in higher plants.…”

Section: Introductionmentioning

confidence: 99%

“…However, this simple gaseous molecule is also involved with other elemental processes throughout the plant's life cycle, including seed germination (Corbineau et al, 2014;Miransari and Smith, 2014;Wilson et al, 2014), root initiation and development (Ivanchenko et al, 2008;Lima et al, 2009;Huang et al, 2013), floral development (O'Neill, 1997;Wuriyanghan et al, 2009), sexual determination (Iwahori et al, 1970;Yamasaki et al, 2001;Salman-Minkov et al, 2008), fruit ripening (Giovannoni, 2001; Barry and Giovannoni, 2007;Lim et al, 2007;De Martinis et al, 2015), plant senescence (Kim et al, 2014;De Martinis et al, 2015;Ueda and Kusaba, 2015), and response to biotic and abiotic stresses (Morgan and Drew, 1997;Wang et al, 2007;Lin et al, 2013;Steffens, 2014). Recently, several studies have uncovered evidence of a relationship between Se and ethylene metabolism in plants (Malorgio et al, 2009;Iqbal et al, 2015;Zhu et al, 2017;Hajiboland et al, 2019;Malheiros et al, 2019). In this vein, Malheiros et al (2019) demonstrated that Se partially inhibits ethylene biosynthesis in roots of rice seedlings.…”

Section: Introductionmentioning

confidence: 99%

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Selenium-Ethylene Interplay in Postharvest Life of Cut Flowers

et al. 2020

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Selenium (Se) is considered a beneficial element in higher plants when provided at low concentrations. Recently, studies have unveiled the interactions between Se and ethylene metabolism throughout plant growth and development. However, despite the evidence that Se may provide longer shelf life in ethylene-sensitive flowers, its primary action on ethylene biosynthesis and cause-effect responses are still understated. In the present review, we discuss the likely action of Se on ethylene biosynthesis and its consequence on postharvest physiology of cut flowers. By combining Se chemical properties with a dissection of ethylene metabolism, we further highlighted both the potential use of Se solutions and their downstream responses. We believe that this report will provide the foundation for the hypothesis that Se plays a key role in the postharvest longevity of ethylene-sensitive flowers.

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Selenium downregulates auxin and ethylene biosynthesis in rice seedlings to modify primary metabolism and root architecture

Cited by 49 publications

References 56 publications

Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

Photosynthetic apparatus performance and anatomical modulations of Alcantarea imperialis (Bromeliaceae) exposed to selenium during in vitro growth

Selenium-Ethylene Interplay in Postharvest Life of Cut Flowers

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