Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection

Abstract

Metabolic oligosaccharide engineering (MOE) is a classical chemical approach to perturb, profile and perceive glycans in physiological systems, but probes upon bioorthogonal reaction require accessibility and the background signal readout makes it challenging to achieve glycan quantification. Here we develop SeMOE, a selenium-based metabolic oligosaccharide engineering strategy that concisely combines elemental analysis and MOE,enabling the mass spectrometric imaging of glycome. We also demonstrate that the new-to-nature SeMOE probes allow for detection, quantitative measurement and visualization of glycans in diverse biological contexts. We also show that chemical reporters on conventional MOE can be integrated into a bifunctional SeMOE probe to provide multimodality signal readouts. SeMOE thus provides a convenient and simplified method to explore the glyco-world.

LRRC25 expression during physiological aging and in mouse models of Alzheimer’s disease and iPSC-derived neurons

“…CCF-STTG1 cells are astrocyte-like cells isolated from a patient with astrocytoma which have been successfully used by multiple investigators as a model of astrocyte ( Elfakhri et al, 2019 ; Sawmiller et al, 2019 ; Tian et al, 2022 ). HMC3 is a microglial cell with a macrophage-like morphology isolated from the brain of a patient and has been authenticated by multiple investigators ( Madsen et al, 2023 ; Pang et al, 2023 ; Tian et al, 2023 ). NT2 cells are clonally derived, pluripotent human embryonal carcinoma cell lines isolated from a male carcinoma patient ( Lee and Andrews, 1986 ).…”