Selenium Ameliorates Acetaminophen-Induced Oxidative Stress via MAPK and Nrf2 Pathways in Mice

Indumathi, Mylanayakanahosahalli Chandrashekar; Swetha, Kamatam; Abhilasha, Kandahalli Venkataranganayaka; Siddappa, Shiva; Kumar, Shivamadhaiah Manjula; Prasad, Govinda Keerthi; Chen, Chu-Huang; Marathe, Gopal Kedihithlu

doi:10.1007/s12011-023-03845-3

Cited by 5 publications

(3 citation statements)

References 98 publications

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“…Ahr is also associated with the oxidative stress response, which includes inflammation, antioxidative and prooxidative enzymes, cytochrome P450, and the mediated oxidative stress response [62]. SOD, CAT, GSH-PX and MPO, as the first line of defence against antioxidative enzymes, are important biochemical parameters for antioxidative defence, and they play important roles in maintaining the antioxidative status under conditions of oxidative stress [63]. The gene expression of these antioxidative enzymes was mediated by the Keap1/Nrf2 and Ahr pathways and was promoted in the 5 ′ -IMP-supplemented treatments compared with control treatments, which agreed with the results of the enzyme activities.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Inosine 5′-Monophosphate Supplementation on the Growth Performance and Salinity and Oxidative Stress Resistance of Gibel Carp (Carassius auratus gibelio)

Hua,

Zhang,

Liu

et al. 2024

Antioxidants

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An 88-day feeding trial was conducted to evaluate the effects of dietary inosine 5′-monophosphate (5′-IMP) on the growth performance and salinity and oxidative stress resistance in the juvenile gibel carp CAS III (Carassius auratus gibelio; initial body weight: 7.48 g). Four isonitrogenous and isoenergetic diets containing exogenous 5′-IMP were formulated. P1, P2, P3 and P4 were diets containing 5′-IMP at four concentrations (0, 1, 2 and 4 g kg−1). The four diets were randomly allotted to triplicate tanks in a recirculating system. After the feeding trial, six fish per tank were netted randomly and placed into 12‰ saline water to test their response to salinity stress. The results indicated that the feed conversion rate was enhanced by dietary supplementation with 5′-IMP. The appetite, plasma neuropeptide Y level and feeding rate of the P3 group were lower than those in the control treatment group. Dietary supplementation with 5′-IMP improved the osmoregulatory adaptation of gibel carp under acute salinity stress. Six hours after the salinity stress treatment, in the dietary 5′-IMP treatment group, the plasma cortisol and K+ concentrations were lower and the Na+/K+-ATPase activity was greater than that in the control group. Dietary supplementation with 5′-IMP promoted the expression of the glucocorticoid receptors NKA-α1b and NKCC and retarded the expression of Hsp70 in P4-treated gill filaments and kidneys. Dietary supplementation with 5′-IMP resulted in a stable oxidative-stress-resistant phenotype characterized by increased levels of cellular antioxidants, including SOD, catalase, glutathione peroxidase, glutathione reductase and MPO. The above results of the current study demonstrate that supplementation of 5′-IMP can promote feed utilization and have positive influences on the salinity and oxidative stress resistance of gibel carp.

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Section: Discussionmentioning

confidence: 99%

Effects of Dietary Inosine 5′-Monophosphate Supplementation on the Growth Performance and Salinity and Oxidative Stress Resistance of Gibel Carp (Carassius auratus gibelio)

Hua,

Zhang,

Liu

et al. 2024

Antioxidants

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“…In addition, the MAPK signaling pathway enriched by network toxicology is also closely related to liver inflammatory response. The key role of MAPK signaling in drug-induced hepatotoxicity is due to the cascade’s interaction with multiple signaling pathways, as noted in a study [ 33 ]. The results of this study suggested that Arecoline can mediate oxidative stress and inflammatory response by regulating JAK, EGFR, MAPK, CASP3, and other key targets and cause hepatotoxicity through HIF-1, MAPK, and other signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Sun,

Zhang,

Yin

et al. 2023

Toxics

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Arecoline is a pyridine alkaloid derived from areca nut in the Arecaceae family. It has extensive medicinal activity, such as analgesic, anti-inflammatory, and anti-allergic. However, the toxicity of Arecoline limits its application. Most current studies on its toxicity mainly focus on immunotoxicity, carcinogenesis, and cancer promotion. However, there are few systematic studies on its hepatotoxicity and mechanisms. Therefore, this research explored the mechanism of hepatotoxicity induced by Arecoline in rats and analyzed endogenous metabolite changes in rat plasma by combining network toxicology with metabolomics. The differential metabolites after Arecoline exposure, such as D-Lysine, N4-Acetylaminobutanal, and L-Arginine, were obtained by metabolomics study, and these differential metabolites were involved in the regulation of lipid metabolism, amino acid metabolism, and vitamin metabolism. Based on the strategy of network toxicology, Arecoline can affect the HIF-1 signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway, and other concerning pathways by regulating critical targets, such as ALB, CASP3, EGFR, and MMP9. Integration of metabolomics and network toxicology results were further analyzed, and it was concluded that Arecoline may induce hepatotoxicity by mediating oxidative stress, inflammatory response, energy and lipid metabolism, and cell apoptosis.

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“…Ca 2+ and ROS signal induced by abiotic stresses can activate the mitogen − activated protein kinas (MAPK) signaling(Chen et al, 2021;Mo et al, 2021). In mice, selenium was found to regulate the ROS-mediated MAPK pathways(Indumathi et al, 2023), while there is little knowledge about the relationship between selenium and MAPK cascade in plants. In this study, we found that selenite treatment obviously promoted the expressions of different MAPK cascade genes, including MAPKs, MPKKs and MAPKKKs in C. moschata.…”

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confidence: 99%

Exploring the effects of selenite treatment on pumpkin (Cucurbita moschata) seedlings by physiology and transcriptome analysis

wang,

Sun,

Wang

et al. 2024

Preprint

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Pumpkin is widely used as a functional food for people and rootstock for other Cucurbitaceae crops. Se-enriched pumpkin can be used as an important Se source for humans. However, the boundary between beneficial and phytotoxic levels of Se is narrow. Here, effects of selenite with different concentrations (2–80 μM) on pumpkin growth and Se accumulation in pumpkin seedlings were explored. Our results revealed that Se accumulations in both shoots and roots of C. moschata were closely related to selenite doses. Selenite seemed to have no positive effects on C. moschata growth. The root activity increased at 2–20 μM of selenite, but significantly decreased at higher doses. High concentrations of selenite (above 40 μM) resulted in serious O2•– accumulation, growth inhibiting and even symptoms of toxicity, which could be alleviated by melatonin. Transcriptome analysis revealed that a series of defense mechanisms, including Ca2+ signaling, ET and JA signaling, antioxidant defense systems, MAPK cascade, proteostasis protection, could be activated in roots of C. moschata in response to 40 μM selenite. Additionally, we identified some important genes probably involved in Se absorption and conversion in C. moschata, such as PHT, SULTR, APS, APK, APR, SiR, GR, and CS genes. These results may help our understanding of the physiological and molecular mechanisms of Se accumulation and tolerance to Se toxicity in C. moschata, which may provide genetic resources and a theoretical basis for the breeding and cultivation of Se-rich pumpkin or rootstocks.

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Selenium Ameliorates Acetaminophen-Induced Oxidative Stress via MAPK and Nrf2 Pathways in Mice

Cited by 5 publications

References 98 publications

Effects of Dietary Inosine 5′-Monophosphate Supplementation on the Growth Performance and Salinity and Oxidative Stress Resistance of Gibel Carp (Carassius auratus gibelio)

Effects of Dietary Inosine 5′-Monophosphate Supplementation on the Growth Performance and Salinity and Oxidative Stress Resistance of Gibel Carp (Carassius auratus gibelio)

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Exploring the effects of selenite treatment on pumpkin (Cucurbita moschata) seedlings by physiology and transcriptome analysis

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