Selenium accumulation and biotransformation in Streptococcus, Lactococcus, and Enterococcus strains

Hyršlová, Ivana; Kaňa, Antonín; Kantorová, Věra; Krausová, Gabriela; Mrvikova, Iva; Doskočil, Ivo

doi:10.1016/j.jff.2022.105056

Cited by 12 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The adherence of isolates from the genera Limosilactobacillus, Lactobacillus, and Lacticaseibacillus to mixed human colon adenocarcinoma cell lines Caco-2 (ATCC HTB-37) and HT-29 (ATCC HTB 38; American Type Culture Collection, Rockville, MD, USA) was tested according to the method described by Musilova et al [23]. A mixture of Caco-2 and HT-29 cells (9:1) prepared as described above was seeded in 24-well plates at a final concentration of 4 × 10 4 cells/mL.…”

Section: Adherence Assaymentioning

confidence: 99%

Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Hyrslova,

Drab,

Cihlar

et al. 2023

Fermentation

Self Cite

View full text Add to dashboard Cite

Infant feces and breast milk are sources of lactic acid bacteria with interesting functional and technological properties. Therefore, we focused on the isolation and characterization of new potential probiotic strains from infant feces and breast milk. Nine strains were identified based on 16S rRNA sequences of Lacticaseibacillus rhamnosus, Limosilactobacillus reuteri, and Lactobacillus gasseri. Subsequently, these isolates were evaluated for their safety (antibiotic resistance, hemolytic, and enzymatic activity) and functional properties (gastrointestinal condition tolerance, hydrophobicity, auto-aggregation, adhesion to Caco-2/HT-29 cell lines, antimicrobial and antioxidant properties, and the production of exopolysaccharides). All the isolates were non-hemolytic and resistant to streptomycin, kanamycin, and vancomycin. They also demonstrated antimicrobial activity against five selected pathogens (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella sp., and Enterococcus faecalis) and the production of exopolysaccharides. Isolates Lacticaseibacillus rhamnosus S1F and S6C together with Lactobacillus gasseri M8C showed the highest ability to survive 3.0 g/L of bile salts and a good adhesion property, which is important for gastrointestinal tract (GIT) colonization or the inhibition of pathogens. Following in vivo characterization, three strains S1F and S6C from infant feces, and M6C from breast milk, showed probiotic properties with potential applications in the food industry.

show abstract

Section: Adherence Assaymentioning

confidence: 99%

Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Hyrslova,

Drab,

Cihlar

et al. 2023

Fermentation

Self Cite

View full text Add to dashboard Cite

show abstract

“…The tolerance of LAB to different Se concentrations varies depending on the strain used [25]. Thus, the study the influence of different Se concentrations (0, 5, 10, 20, 40 µg/mL) on the growth of six LAB strains.…”

Section: Discussionmentioning

confidence: 99%

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Zan,

Chen,

Zhang

et al. 2024

Fermentation

View full text Add to dashboard Cite

Considerable progress has been achieved in the bioaccumulation and transformation of selenium (Se)-enriched lactic acid bacteria (LAB). However, research on the effects of Se on the structure and probiotic potential of LAB is relatively limited. In this study, six industrial LAB strains, including Lactobacillus plantarum 21,805, Lactobacillus paracasei 20,241, Lactobacillus fermentum 21,828, Lactobacillus casei 23,185, Lactobacillus acidophilus 6064, and Lactobacillus plantarum 6076, were cultured in De Man, Rogosa, and Sharpe (MRS) medium containing Se for three generations. High-Se LAB was screened based on bacterial biomass, viable bacterial count, and Se content. Their structural characteristics were analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The results indicated that LAB growth was influenced by the Se environment, with high Se (20 μg/mL) inhibiting it. At a Se concentration of 10 μg/mL, LAB maintained integrity and exhibited a high Se enrichment ability, with a Se enrichment of 4.88 ± 0.39 mg/g. The intracellular Se existed in an amorphous or non-crystalline form. Furthermore, Se-enriched LAB exhibited enhanced probiotic properties, including tolerance to simulated gastrointestinal fluids, acid resistance, bile salt resistance, and hydrophobicity. Therefore, Se-enriched LAB are expected to develop new functional foods or dietary supplements for human and animal consumption.

show abstract

“…39 Se content in the samples was determined using an ICP-MS 7850 (Agilent), employing the same operational conditions as those used for selenized bacteria and encapsulates. 4…”

Section: Supplementation Of Beef Cattle Feed With Encapsulated Seleni...mentioning

confidence: 99%

“…Lactic acid bacteria (LAB), yeast, and microalgae possess the capability to metabolize inorganic selenium (Se), leading to the bioaccumulation of this element in various organic forms, including the bioavailable selenomethionine. [1][2][3][4] Compared to inorganic forms of Se, selenomethionine has been shown to exhibit lower toxicity and higher bioavailability. 3,5,6 Se deficiency in animals can lead to nutritional muscular dystrophy, 5,[7][8][9] characterized by muscular stiffness, weakness, and cardiac complications.…”

Section: Introductionmentioning

confidence: 99%

Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation

Schwingel Henn,

Cima,

Bettanin

et al. 2024

Biotech and App Biochem

View full text Add to dashboard Cite

This study aimed to assess the technical feasibility of incorporating selenized Lactobacillus spp. microencapsulated via spray drying into cattle feed. Gum Arabic and maltodextrin were used as encapsulating agents. The encapsulation process was carried out with a drying air flow rate of 1.75 m3/min, inlet air temperature of 90°C, and outlet air temperature of 75°C. The viability of the encapsulated microorganisms and the technological characteristics of the obtained microparticles were evaluated. Microorganisms were incorporated into beef cattle feed to supplement their diet with up to 0.3 mg of Se per kilogram of feed. The encapsulated particles, consisting of a 50/50 ratio of gum Arabic/maltodextrin at a 1:20 proportion of selenized biomass to encapsulant mixture, exhibited superior technical viability for application in beef cattle feed. Supplemented feeds displayed suitable moisture, water activity, and hygroscopicity values, ensuring the preservation of viable microorganisms for up to 5 months of storage, with an approximate count of 4.5 log CFU/g. Therefore, supplementing beef cattle feed with selenized and microencapsulated lactic acid bacteria represents a viable technological alternative, contributing to increased animal protein productivity through proper nutrition.

show abstract

Selenium accumulation and biotransformation in Streptococcus, Lactococcus, and Enterococcus strains

Cited by 12 publications

References 30 publications

Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation

Contact Info

Product

Resources

About