Selektiver, hoch empfindlicher und schneller Nachweis genomischer DNA mit gesteuerten Materialien am Beispiel von <i>Mycoplasma</i>

Climent, Estela; Mondragón, Laura; Martínez‐Máñez, Ramón; Sancenón, Félix; Marcos, Mercedes; Murguía, José Ramón; Amorós, Pedro; Rurack, Knut; Pérez‐Payá, Enrique

doi:10.1002/ange.201302954

Cited by 12 publications

(3 citation statements)

References 42 publications

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“…[69] Thes ame principle was applied to several other analytes by simply using ad ifferent aptamer.T his also enabled, for example,t he detection of genomic DNAdown to 50 DNAcopies mL À1 ,which is similar to PCR-based approaches but enables afaster detection with less sources of error because of the omission of analyte amplification. [94] Besides the detection of biological molecules,t hese nanocontainers also enable the quantification of chemical substances.F or example,b isphenol A( BPA) was successfully detected down to 3.5 mm.T his is comparable to other setups based on aptamer recognition and sufficient to detect hazardous amounts of BPAi nw ater, but electrochemical sensors based on rGO electrodes have been reported to work down to nm levels. [93] Similar approaches have also been reported with capped gold nanocages.…”

Section: Angewandte Chemiementioning

confidence: 99%

Nanocontainers for Analytical Applications

2019

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Nanocontainers such as mesoporous silica particles and polymersomes are versatile structures containing holes or pores which are used for the entrapment of small molecules and the introduction of specific functionalities. They are widely applied in drug delivery, biomedicine, bioreactors, and analytical applications. In the last case, nanocontainers usually serve as amplification systems. They are hence synthesized to entrap signaling molecules and to bear functional moieties at the outer surface, which in turn enable specific analyte recognition and control of the nanocontainer pore permeability. This Review outlines the most important nanocontainer materials and discusses their synthesis, surface chemistry modifications, and strategies for molecule entrapment. Their advantages, challenges, and limitations are critically discussed in view of other common signal amplification strategies for different assay formats and various detection methods.

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Section: Angewandte Chemiementioning

confidence: 99%

Nanocontainers for Analytical Applications

2019

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“…8-mal niedrigeren LOD-Wert entspricht als mit einem kommerziell erhältlichen ELISA-Kit. [94] Abgesehen von der Detektion biologischer Moleküle ermçglichen diese Nanocontainer auch die Quantifizierung von chemischen Substanzen. Hierbei wurden nach Verdrängen der Polystyrolpartikel durch den Analyten eingeschlossene Glucosemoleküle freigesetzt.…”

Section: Aufsätzeunclassified

“…[69] Das gleiche Prinzip wurde fürz ahlreiche andere Analyten genutzt, indem lediglich ein anderes Aptamer verwendet wurde.Dies ermçglichte beispielsweise die Detektion von genomischer DNAm it bis zu 50 DNA-Kopien mL À1 ,w as in einem ähnlichen Bereich liegt wie Polymerasekettenreaktionsansätze.A llerdings ermçglicht dieser Ansatz eine schnellere Detektion mit weniger Fehlerquellen infolge der fehlenden Amplifizierung des Analyten. [94] Abgesehen von der Detektion biologischer Moleküle ermçglichen diese Nanocontainer auch die Quantifizierung von chemischen Substanzen. Zum Beispiel wurde Bisphenol A(BPA) in einer Konzentration von bis zu 3.5 mm detektiert.…”

Section: Aufsätzeunclassified

Nanocontainer in der Analytik

2019

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Nanocontainer, wie mesoporöse Siliciumoxidpartikel und Polymersomen, sind vielseitige Strukturen, die Löcher oder Poren enthalten, die für den Einschluss von niedermolekularen Verbindungen und für die Einführung spezifischer Funktionalitäten verwendet werden. Sie finden weitreichende Anwendungen in Wirkstofftransportsystemen, in der Biomedizin, in Bioreaktoren und in der Analytik. Im letzten Fall fungieren die Nanocontainer normalerweise als Signalverstärker. Dafür werden sie so synthetisiert, dass sie Signalmoleküle einschließen und funktionelle Gruppen an ihrer Oberfläche tragen. Diese ermöglichen wiederum die spezifische Erkennung von Analyten und kontrollieren die Durchlässigkeit der Nanocontainerporen. Dieser Aufsatz gibt einen Überblick über die wichtigsten Nanocontainermaterialien und diskutiert deren Herstellung, die Oberflächenchemie und ‐modifizierungen sowie Strategien für den Einschluss von Molekülen. Außerdem werden die Vorteile, Herausforderungen und Grenzen in Bezug auf andere gängige Signalverstärkungsstrategien für verschiedene Assayformate und Detektionsmethoden in (bio)analytischen Anwendungen kritisch betrachtet.

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Programmed Synthesis by Stimuli‐Responsive DNAzyme‐Modified Mesoporous SiO₂ Nanoparticles

et al. 2015

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DNAzyme-capped mesoporous SiO 2 nanoparticles (MP SiO 2 NPs) are applied as stimuli-responsive containers for programmed synthesis.T hree types of MP SiO 2 NPs are prepared by loading the NPs with Cy3-DBCO (DBCO = dibenzocyclooctyl), Cy5-N 3 ,a nd Cy7-N 3 ,a nd capping the NP containers with the Mg 2+ ,Z n 2+ ,a nd histidine-dependent DNAzyme sequences,respectively.Inthe presence of Mg 2+ and Zn 2+ ions as triggers,the respective DNAzyme-capped NPs are unlocked, leading to the "click"reaction product Cy3-Cy5. In turn, in the presence of Mg 2+ ions and histidine as triggers the second set of DNAzyme-capped NPs is unlocked leading to the Cy3-Cy7 conjugated product. The unloading of the respective NPs and the time-dependent formation of the products are followed by fluorescence spectroscopy( FRET). Ad etailed kinetic model for the formation of the different products is formulated and it correlates nicely with the experimental results.The entrapment of substrates in the pores of mesoporous SiO 2 nanoparticles (MP SiO 2 NPs) and their controlled release by means of stimuli-responsive caps attracts substantial research interest. [1,2] Different applications of these NPs were suggested including controlled drug delivery, [3][4][5] sensing, [6] imaging, [7,8] and switchable catalysis. [9][10][11] Va rious stimuli were applied to unlock the pore caps and release the loaded substrates.T hese included pH, [12][13][14][15] redox reagents, [16][17][18] photonic signals, [19,20] and enzymes [21][22][23][24] as triggers.A lso,t he cooperative operation of gated nanoparticles was recently reported.[25] One class of stimuli-responsive MP SiO 2 NPs includes nucleic-acid-capped NPs.Bulky duplex nucleic acids, hairpin systems,p H-sensitive nucleic acids,a nd G-quadruplexes were used to cap the pores.Unlocking the nucleic acid gates through the formation of aptamer-ligand complexes, [26] pH-induced reconfiguration of i-motif structures to random coils, [27] crown-ether-stimulated separation of K + -ion-stabilized G-quadruplexes, [28] or enzymatic digestion of DNAcaps were reported.[29] Thep resent study introduces the novel application of stimuli-responsive MP SiO 2 NPs for programmed synthesis.W eintroduce metal-ion-dependent catalytic nucleic acids (DNAzymes) as functional caps of MP SiO 2 NPs loaded with different reagents.S pecific triggers lead to the dictated release of the loads and their programmed reactions.T he study provides ag eneral innovative paradigm for programmed synthesis in the presence of functional nanoparticle assemblies. Sequence-specific cofactor-dependent DNAzymes were used as stimuli-responsive capping units of MP SiO 2 NPs for the controlled release of drugs.[30] Thep resent study introduces the use of cofactor-dependent DNAzyme-capped mesoporous SiO 2 NPs for as timuli-dictated copper-free synthesis of "click-chemistry" products [31] (Figure 1a). This is demonstrated using amixture of MP SiO 2 NPs loaded with different reactants,t hat is,C y3-DBCO (1)( DBCO = dibenzocyclooctyl), Cy5-N 3 (2), and...

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Selektiver, hoch empfindlicher und schneller Nachweis genomischer DNA mit gesteuerten Materialien am Beispiel von Mycoplasma

Cited by 12 publications

References 42 publications

Nanocontainers for Analytical Applications

Nanocontainers for Analytical Applications

Nanocontainer in der Analytik

Programmed Synthesis by Stimuli‐Responsive DNAzyme‐Modified Mesoporous SiO₂ Nanoparticles

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Selektiver, hoch empfindlicher und schneller Nachweis genomischer DNA mit gesteuerten Materialien am Beispiel von Mycoplasma

Cited by 12 publications

References 42 publications

Nanocontainers for Analytical Applications

Nanocontainers for Analytical Applications

Nanocontainer in der Analytik

Programmed Synthesis by Stimuli‐Responsive DNAzyme‐Modified Mesoporous SiO2 Nanoparticles

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Product

Resources

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Programmed Synthesis by Stimuli‐Responsive DNAzyme‐Modified Mesoporous SiO₂ Nanoparticles