Selektive dsDNA‐gesteuerte Bildung von Kupfer‐Nanopartikeln in Lösung

Rotaru, Alexandru; Dutta, Sonia; Jentzsch, Elmar; Gothelf, Kurt V.; Mokhir, Andriy

doi:10.1002/ange.200907256

Cited by 15 publications

(18 citation statements)

References 27 publications

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“…These data are in accordance with previously published results [14]. By fluorescence spectroscopy,w ec ould observe that the formationo f CuNPs is finished after 15 min of incubation at room temperature.Addition of ahigher amountofCuSO 4 (twice the initial amount)did not change the shape or the maximum intensity of the emissions pectra.…”

supporting

confidence: 94%

“…Fluorescence data did not showt he specific emissionb and characteristic for the formationo fC uNPs in solution (data not presented), we could only investigate the sample by TEM. We could observe that, in comparison to bare DNA-stabilized SWCNTs, the obtaineds tructures represent carbon nanotubes covered withathin and uneven layer of metal without clear formation of nanoparticles.T his result is in accordance with the previously reported dataw here the initial binding of Cu 2þ to dsDNAi sd isturbingr ather than facilitating the transformation of Cu 2þ to Cu 0 due to the stabilization of Cu 2þ by the N-and Oatoms of the nucleobases [14].…”

supporting

confidence: 93%

“…Mokhir and co-workers [ 14]r eported for the first time that dsDNAc an act as an efficient template for the formation of fluorescentCunanoparticles (CuNPs)utilizing Cu 2þ and subsequently sodium ascorbate as reducing agent, whereas random ssDNA does not support nanoparticlef ormation. Later, Wang and co-workers [ 15]a lso reported that only very specific ssDNAs equences could also serve as templates for CuNP formationi na queous buffersolution under the sameconditions.…”

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confidence: 99%

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DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

Ursu

Clima

Hejesen

et al. 2015

Helvetica Chimica Acta

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An ew and facile methodf or the preparation of single-walled carbon nanotubes( SWCNTs) decorated with Cu nanoparticles (CuNPs)f ormed on ad ouble-stranded DNAt emplate in aqueous solution has been developed.Aspecially designed synthetic DNAsequence,containing asingle-stranded domain for the dispersion of carbon nanotubes and double-strandeddomainsfor the selective growth of CuNPs,w as utilized. Thef inal SWCNT/CuNP hybrids were characterized using fluorescence spectroscopy and transmission electron microscopy.T he analyses clearly demonstrated the selective formation of uniform CuNPs on the carbon nanotubescaffold.Introduction. -Novel 1D single-walled carbon nanotubes (SWCNTs)/nanoparticle hybrid materials,i nw hich SWCNTs are oftenu sed as scaffolds for the assemblyo f nanoparticles,a re of great importance due to their considerableh ardness and toughness [1].S WCNT/metal nanoparticle hybrids, especially transition metal nanoparticle-decoratedS WCNTs,h ave aroused great interest because of their vast applications in sensor devices [2], photoelectrochemicalc ells [3], as catalytics upporters [4], and in surface-enhanced Raman spectroscopy [5].Controlled decoration of SWCNTsw ith metal nanoparticles modifies their electronic structure resulting in better electroanalytical performance due to the synergetic effect of the two materials compared to bare metal nanoparticles [6], making important not only fundamental studies of the interactionsb etween the carbon matrix and the metallic nanoparticles, but also the aspects of their broad potential applications.Currently,v arious methods are being explored, generallyb eing divided into covalent or noncovalent attachment of certain metal nanoparticles onto carbon nanotubes [7 -12].Covalent deposition requires prefunctionalization of the nanotubes by modifying their surface.Inmany cases,itinvolves acid treatment to create COOH, C¼O, or OH groups for subsequent anchoring of metal nanoparticles [8]. Theh arsh acidic treatment of SWCNTs may introduce many extensive defects in the sidewall, thusconsiderablyperturbing the mechanical and electronic performance of the tubes. On the other hand, the noncovalentapproach maintains the properties of the nanotube, the carbon scaffold being minimally perturbed by utilizing functional compounds,such as organic molecules [9], polyelectrolytes [10], and single-stranded DNA( ssDNA)

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supporting

confidence: 93%

mentioning

confidence: 99%

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DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

Ursu

Clima

Hejesen

et al. 2015

Helvetica Chimica Acta

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“…Among the reported DNA‐templated metal nanoclusters, DNA‐templated copper nanoclusters (CuNCs) exhibit some excellent properties. Firstly, synthesis of DNA‐templated CuNCs is extremely fast and convenient (within 10 min at room temperature) ,. Secondly, CuSO 4 and ascorbate (Vc) are the precursors for preparation of CuNCs, and these two chemicals are relatively inexpensive and readily available.…”

Section: Introductionmentioning

confidence: 99%

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu²⁺ and Ascorbate as Signal Developers

et al. 2019

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We report herein a label-free and turn-on fluorescence approach for detecting DNA polymerase activity. In the presence of DNA polymerase, the DNA primer could be elongated to produce one long double-stranded DNA (dsDNA). Then, the produced dsDNA acted as the template for in-situ synthesizing fluorescent copper nanoclusters (CuNCs) via Cu 2 +ascorbate reaction (10 min at room temperature), and the fluorescent intensity of the CuNCs was used to quantify the activity of DNA polymerase. In this strategy, cheap CuSO 4 and ascorbate acted as fluorescence developers, so this method is low-cost and easy to carry out. Interestingly, based on the sequence-dependent fluorescence of DNA-templated CuNCs, we rationally designed the sequence of DNA substrate to decrease the background and increase the signal of this assay. Therefore, this proposed method exhibited a high sensitivity toward DNA polymerase, and the limit of detection was 3.7 × 10 -7 U/μL for Klenow Fragment (KF) polymerase. The strategy suggests a "mix-and-detect" homogenous assay format without precipitation, separation and washing. The whole process (including enzyme reaction and synthesizing CuNCs) can be completed in a single tube. This fluorescence method is lowcost and simple, and shows potential application in some biomedical studies and clinical diagnostics.

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“…Due to quantum confinement, nanomaterials with sizes in the range of 1–100 nm have demonstrated unique properties and applications . Over the past ten years, significant advances in the synthesis, characterization, and applications of a variety of nanomaterials such as gold nanoparticles (AuNPs), silver nanoclusters, copper nanoparticles, quantum dots, magnetic nanoparticles, and carbon quantum dots have blossomed. AuNPs are one of the most popular nanomaterials, mainly because of their easy preparation, flexible conjugation, stability, and biocompatibility .…”

Section: Introductionmentioning

confidence: 99%

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

Wang

et al. 2016

ChemBioChem

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Gold nanoparticles (AuNPs) are useful for diagnostic and biomedical applications, mainly because of their ease in preparation and conjugation, biocompatibility, and size-dependent optical properties. However, bare AuNPs do not possess specificity for targets. AuNPs conjugated with DNA aptamers offer specificity for various analytes, such as proteins and small molecules/ions. Although DNA aptamers themselves have therapeutic and target-recognizing properties, they are susceptible to degradation in vivo. When DNA aptamers are conjugated to AuNPs, their stability and cell uptake efficiency both increase, making aptamer-AuNPs suitable for biomedical applications. Additionally, drugs can be efficiently conjugated with DNA aptamer-AuNPs to further enhance their therapeutic efficiency. This review focuses on the applications of DNA aptamer-based AuNPs in several biomedical areas, including anticoagulation, anticancer, antibacterial, and antiviral applications.

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Selektive dsDNA‐gesteuerte Bildung von Kupfer‐Nanopartikeln in Lösung

Cited by 15 publications

References 27 publications

DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu²⁺ and Ascorbate as Signal Developers

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

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Selektive dsDNA‐gesteuerte Bildung von Kupfer‐Nanopartikeln in Lösung

Cited by 15 publications

References 27 publications

DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

DNA‐Mediated Copper Nanoparticle Formation on Dispersed Single‐Walled Carbon Nanotubes

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu2+ and Ascorbate as Signal Developers

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

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Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu²⁺ and Ascorbate as Signal Developers