Selektion und biotechnische Herstellung von Kulturlösungen mikrobieller Antagonisten zur Unterdrückung phytopathogener Bodenpilze

Huber, Johann; Bochow, H.; Junge, Helmut

doi:10.1002/jobm.3620270907

Cited by 12 publications

(6 citation statements)

References 7 publications

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“…1989 ). However, it has often been reported as an antifungal agent against plant pathogens ( Huber et al . 1987 ; Hebbar et al .…”

Section: Introductionmentioning

confidence: 99%

“…Bacillus subtilis is not a typical rhizobacterium; it also occurs in the soil surrounding the root (Miller et al 1989). However, it has often been reported as an antifungal agent against plant pathogens (Huber et al 1987;Hebbar et al 1991;Milus and Rothrock 1993). Bacillus subtilis A13 was categorized as a plant growth-promoting rhizobacterium (PGPR) by Kloepper et al (1989).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Marten

Smalla

Berg

2000

Journal of Applied Microbiology

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Physiological and molecular fingerprints of biotechnologically relevant rhizobacteria are necessary for registration, patenting, recognition and quality checking of the strains. To characterize the biological control agent, Bacillus subtilis B2g, the strain was compared with other plant‐associated B. subtilis isolates. Phenotypic characterization included biochemical and nutritional properties, in vitro activity and analysis of potential antagonistic mechanisms towards several plant pathogenic fungi. According to the phenotypic characteristics, it was not possible to differentiate the biocontrol agent from the other strains, although the enzymatic fingerprint was unique. Genotypic diversity among the isolates was characterized by molecular fingerprinting methods using REP–PCR (repetitive extragenomic palindromic PCR), and macrorestriction of genomic DNA and electrophoretic separation of DNA fragments by pulsed‐field gel electrophoresis (PFGE). A protocol for PFGE analysis using restriction enzyme SfiI for B. subtilis was developed. PFGE typing of B. subtilis B2g resulted in a unique fingerprint. Therefore, it was possible to differentiate B. subtilis B2g, the biocontrol agent of Phytovit®, from other antifungal B. subtilis isolates.

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“…1989 ). However, it has often been reported as an antifungal agent against plant pathogens ( Huber et al . 1987 ; Hebbar et al .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Marten

Smalla

Berg

2000

Journal of Applied Microbiology

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“…The antifungal activity against F. oxysporum and R. solani of this crude supernatant was determined using the method of Huber 14 . Different amounts of the crude supernatant were added to potato dextrose agar (PDA) at 55°C, mixed well, and poured into Petri dishes (90 × 15 mm).…”

Section: Antifungal Activity Of the Crude Bacterial Supernatant Determentioning

confidence: 99%

Purification and characterization of an antifungal protein from Bacillus subtilis XL62 isolated in Vietnam

Tuyen¹,

Le²,

Nguyen³

et al. 2017

ScienceAsia

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ABSTRACT:Bacillus subtilis is considered as a potential antagonist for fungal control due to its ability to produce a wide range of antibiotics. This study determines the antifungal activity of the native B. subtilis XL62 and we purify, identify, and characterize the antifungal protein. The results show that the growth inhibitory activity to Fusarium oxysporum and Rhizoctonia solani of the crude supernatant of B. subtilis XL62 was proportional to the concentration. At the highest concentration (50%), the growth inhibition reached 90% and 100% for F. oxysporum, and R. solani, respectively. Furthermore, the crude supernatant of B. subtilis XL62 also inhibited the germination and growth of R. solani. The growth of sclerotia was almost completely inhibited at the concentration of 20% and the ability to germinate was lost at the concentration of 50%. Antifungal proteins were isolated from the crude bacterial supernatant using ammonium sulphate precipitation followed by passage over DEAE-cellulose and Biogel P100 columns. The purified protein had an apparent molecular mass of 22 kDa. Its antifungal activity was retained even at 100°C, for 60 min and after treatment with proteinase K (0.5-2.5 µg/ml). The results of protein identification using a MALDI-TOF/TOF mass spectrometer suggest that the purified protein is indeed a chitin-binding protein.

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“…Antifungal activity of the extracellular extracts The extracellular extracts from Serratia marcescens DT3 were obtained by centrifuging the bacterial culture at 17,460 × g for 10 min. The antifungal activity of this extracellular extract was determined using the method of Huber et al (1987). Different amounts of extracellular extracts were added to potato dextrose agar (PDA) at 55 °C, mixed well, and poured into petri dishes (90 × 15 mm).…”

Section: Protein Identificationmentioning

confidence: 99%

“…Three different concentrations of extracellular extracts (20%, 30%, and 50%) of Serratia marcescens DT3 were tested for their antifungal activity against Rhizoctonia solani and Fusarium oxysporum using the method of Huber et al, (1987). The results (Table 1) showed that the extracellular extracts of Serratia marcescens DT3 have significant antifungal activity on both fungal pathogens.…”

Section: Antifungal Activity Of the Extracellular Extracts Of S Marcescens Dt3mentioning

confidence: 99%

Optimization, purification, and characterization of an extracellular antifungal protein from Serratia marcescens DT3 isolated from soil in Vietnam

Tuyen¹,

Nguyen²,

Nguyen³

et al. 2017

Turk J Biol

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Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the extracellular extract was increased by a factor of 1.6-2.9 in comparison with the initial condition. The purified protein had an apparent molecular mass of 55 kDa. Its antifungal activity was retained at 80 °C for 30 min and after having been treated with proteinase K (0.5-4 µg/mL). The results of protein identification using a MALDI-TOF/TOF mass spectrometer suggested that the purified protein is indeed a serine 3-dehydrogenase.

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Selektion und biotechnische Herstellung von Kulturlösungen mikrobieller Antagonisten zur Unterdrückung phytopathogener Bodenpilze

Cited by 12 publications

References 7 publications

Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Purification and characterization of an antifungal protein from Bacillus subtilis XL62 isolated in Vietnam

Optimization, purification, and characterization of an extracellular antifungal protein from Serratia marcescens DT3 isolated from soil in Vietnam

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