Selectivity and sensitivity of molybdenum oxide-polycaprolactone nanofiber composites on skin cancer: Preliminary in-vitro and in-vivo implications

Indrakumar, Janani; Lakra, Rachita; Kiran, Manikantan Syamala; Korrapati, Purna Sai

doi:10.1016/j.jtemb.2018.04.033

Cited by 43 publications

(20 citation statements)

References 22 publications

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“…Since water causes lysis of RBCs, water was used as the negative control, i.e., 100% hemolytic. MoO 3 nanoparticles also did not show any hemolysis which is on par with the previous reports on molybdenum [25]. This clearly shows that the fabricated nanoparticles will not elucidate any immune response in contact with the bloodstream.…”

Section: Hemolytic Assaysupporting

confidence: 77%

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Steering Efficacy of Nano Molybdenum Towards Cancer: Mechanism of Action

Indrakumar

Korrapati

2019

Biol Trace Elem Res

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Conventional cancer therapies possess a plethora of limitations which led to the awakening of nanotechnology and nanomedicine. However, technological success is widely dependent on complete understanding of the complexity and heterogeneity of tumor biology on one hand and nanobiointeractions associated with challenges of synthesis, translation, and commercialization on the other. The present study therefore deals with one such targeted approach aiming at synthesizing, characterizing, and understanding the efficacy of molybdenum oxide nanoparticles. The phase structure, morphology, and elemental composition of the synthesized nanoparticles were characterized using Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopy. The cytotoxicity studies revealed that the IC 50 vales of molybdenum trioxide (MoO 3) particles against skin cancer cells (melanoma and nonmelanoma) were around 200-300 μg. The nanoparticles were found to induce mitochondrial-mediated apoptosis driven by the apoptotic genes such as BAX and Bcl 2. Molybdenum being a cofactor for the majority of metabolic enzymes could have triggered the selective internalization of the nanoparticles which in turn could have modified the granularity of the cytoplasm and subsequently lead to mitochondrial-mediated apoptosis. Further, the anti-angiogenic property of MoO 3 nanoparticles was corroborated using Chick chorioallantoic membrane (CAM) assay and aortic ring assay. Taken together , unraveling the role of MoO 3 nanoparticles in cancer and angiogenesis opens up venues for nano biological intervention of selective cancer cell targeting with minimal damage to the normal cells using natural trace elements that are generally known to influence various metabolic enzymes.

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Section: Hemolytic Assaysupporting

confidence: 77%

“…3d. The Fourier infrared spectroscopy and the Raman spectroscopy were done to analyze the purity of the nanoparticle and have been reported in our previous study [25] conforming the synthesis of MoO 3 nanoparticles.…”

Section: X-ray Photoelectron Spectroscopymentioning

confidence: 99%

Steering Efficacy of Nano Molybdenum Towards Cancer: Mechanism of Action

Indrakumar

Korrapati

2019

Biol Trace Elem Res

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“…Other bioactive substances used for incorporation into PCL-based nanofibers included medicinal herbs such as Aloe vera [61], lawsone, i.e., 2-hydroxy-1,4-naphthoquinone extracted from Henna, endowed with antimicrobial, antiparasitic, Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic… DOI: http://dx.doi.org/10.5772/intechopen.88744 anticancer, and antioxidant activities [22], other plant extracts with wound healing effects, e.g., from Calendula officinalis [65] or Indigofera aspalathoides, Azadirachta indica, Memecylon edule, and Myristica andamanica [66], molybdenum oxide nanoparticles for treating skin cancer [67] or antibiotics, which can be combined with PCL by various manners, e.g., through whey protein [62] or through micelles coating PCL/collagen nanofibers [6].…”

Section: Nanofibers From Synthetic Degradable Polymersmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

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Nanofibrous scaffolds are popular materials in all areas of tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. In this chapter, we focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is an organ with several vitally important functions, particularly barrier, thermoregulatory, and sensory functions. Nanofibrous meshes not only serve as carriers for skin cells but also can prevent the penetration of microbes into wounds and can keep appropriate moisture in the damaged skin. The nanofibrous meshes have been prepared from a wide range of synthetic and nature-derived polymers. This review is concentrated on synthetic non-degradable and degradable polymers, which have been explored for skin tissue engineering and wound healing. These synthetic polymers were often combined with natural polymers of the protein or polysaccharide nature, which improved their attractiveness for cell colonization. The nanofibrous scaffolds can also be loaded with various bioactive molecules, such as growth factors, hormones, vitamins, antioxidants, antimicrobial, and antitumor agents. In advanced tissue engineering approaches, the cells on the nanofibrous scaffolds are cultured in dynamic bioreactors enabling appropriate mechanical stimulation of cells and at air-liquid interface. This chapter summarizes recent results achieved in the field of nanofiber-based skin tissue engineering, including results of our research group.

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“…Recognition of an appropriate carrier system for the NPs would lessen various challenges. In this way, MoO 3 could be used to fabricate a nanofibrous support with appropriate physicochemical properties to purposefully target cancer cells in order to overcome the challenges and lessen dosage and side effects [52–54].…”

Section: Nanotechnologymentioning

confidence: 99%

Impact of Molybdenum Compounds as Anticancer Agents

Odularu

Ajibade

Mbese

2019

Bioinorganic Chemistry and Applications

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The aim of this mini review was to report the molybdenum compound intervention to control cancer disease. The intervention explains its roles and progress from inorganic molybdenum compounds via organomolybdenum complexes to its nanoparticles to control oesophageal cancer and breast cancer as case studies. Main contributions of molybdenum compounds as anticancer agents could be observed in their nanofibrous support with suitable physicochemical properties, combination therapy, and biosensors (biomarkers). Recent areas in anticancer drug design, which entail the uses of selected targets, were also surveyed and proposed.

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Selectivity and sensitivity of molybdenum oxide-polycaprolactone nanofiber composites on skin cancer: Preliminary in-vitro and in-vivo implications

Cited by 43 publications

References 22 publications

Steering Efficacy of Nano Molybdenum Towards Cancer: Mechanism of Action

Steering Efficacy of Nano Molybdenum Towards Cancer: Mechanism of Action

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Impact of Molybdenum Compounds as Anticancer Agents

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