Selectively RNA interaction by a hnRNPA/B-like protein at presynaptic terminal of squid neuron

Lopes, Gabriel S; Brusco, Janaína; Rosa, José César; Larson, Roy Edward; Lico, Diego Torrecillas Paula

doi:10.1007/s10158-020-00248-0

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…Indeed, we speculate that the different subcellular localization of hnRNP A1B may be relevant to neuronal cytoplasmic functions and note that the hnRNP A1B puncta we observe in neuronal processes/axons is similar to the RNP particles observed in neurons (Knowles et al, 1996;Köhrmann et al, 1999;Tang et al, 2001;Huang et al, 2003;Zhang et al, 2006). Moreover, squid hnRNP A1B orthologous proteins are enriched in synaptosomes (Lopes et al, 2020). Taken together, we speculate that the cytoplasmic pool of hnRNP A1B in motor neurons is implicated in neuronal mRNA transport and/or translation (Kiebler and Bassell, 2006;Pushpalatha and Besse, 2019).…”

Section: Discussionsupporting

confidence: 60%

hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated

et al. 2021

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RNA binding proteins (RBPs) play a key role in cellular growth, homoeostasis and survival and are tightly regulated. A deep understanding of their spatiotemporal regulation is needed to understand their contribution to physiology and pathology. Here, we have characterized the spatiotemporal expression pattern of hnRNP A1 and its splice variant hnRNP A1B in mice. We have found that hnRNP A1B expression is more restricted to the CNS compared to hnRNP A1, and that it can form an SDS-resistant dimer in the CNS. Also, hnRNP A1B expression becomes progressively restricted to motor neurons in the ventral horn of the spinal cord, compared to hnRNP A1 which is more broadly expressed. We also demonstrate that hnRNP A1B is present in neuronal processes, while hnRNP A1 is absent. This finding supports a hypothesis that hnRNP A1B may have a cytosolic function in neurons that is not shared with hnRNP A1. Our results demonstrate that both isoforms are differentially expressed across tissues and have distinct localization profiles, suggesting that the two isoforms may have specific subcellular functions that can uniquely contribute to disease progression.

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Section: Discussionsupporting

confidence: 60%

hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated

et al. 2021

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“…The complexity of subcellular domains in neurons and their distances of nucleus demand a spatial and temporal control of protein synthesis. We have identified a novel member of hnRNP A/B type, of 65 kDa, in the presynaptic terminal of squid neuron [30][31][32]. Squid p65 was phylogenetically conserved hnRNP type A/B protein in mammals [33].…”

Section: Immunohistochemistry and Immunofluorescence Studies: Squid O...mentioning

confidence: 98%

The Biological and Structural Organization of the Squid Brain

Lico¹

2023

Animal Models and Experimental Research in Medicine

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Marine invertebrate models (squid, sepia, and octopus) made important contributions to description mammals’ nervous system. Being a very simple nervous system relatively easy to be manipulated experimentally and visualized by simple microscope or magnifying glass, the giant synapses at stellate ganglion and the large synaptosomes prepared from the squid photoreceptor neurons served as an attractive model to Histology and Anatomy studies. This sophisticated nervous system has elucidated synaptic transmission in detail with their numerous proteins at presynaptic terminal, synaptic vesicle biogenesis, neurotransmitter secretion, vesicle recycling and, allowed the study of postsynaptic complex with their membranes receptors. However, there are few studies with biochemical and molecular approaches, which lead to a better understanding of their physiological functions and verify operation of such nervous system.

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“…In particular, A3 puncta were found in neurites from cultured hippocampal mouse neurons, but these puncta were distinct from A2-containing RNPs, indicating a role in trafficking of specific transcripts in neurons [ 39 ]. Quite recently, Lopes et al [ 282 ] studied squid A3, showing that it forms a homodimer in RNA-rich regions in synaptosomes of squid neurons, and binds specific mRNAs at the synaptic terminal. Thus, the authors concluded that A3 plays a role in RNP formation for site-specific RNA processing [ 282 ].…”

Section: Hnrnp A3mentioning

confidence: 99%

“…Quite recently, Lopes et al [ 282 ] studied squid A3, showing that it forms a homodimer in RNA-rich regions in synaptosomes of squid neurons, and binds specific mRNAs at the synaptic terminal. Thus, the authors concluded that A3 plays a role in RNP formation for site-specific RNA processing [ 282 ]. A3 was also recently shown to play a critical role in neural development in A3 knockout mice and under transient knockdown in mouse and human brain tissue [ 283 ].…”

Section: Hnrnp A3mentioning

confidence: 99%

hnRNP A/B Proteins: An Encyclopedic Assessment of Their Roles in Homeostasis and Disease

Thibault

Kalyaanamoorthy

Clarke

et al. 2021

Biology

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The hnRNP A/B family of proteins is canonically central to cellular RNA metabolism, but due to their highly conserved nature, the functional differences between hnRNP A1, A2/B1, A0, and A3 are often overlooked. In this review, we explore and identify the shared and disparate homeostatic and disease-related functions of the hnRNP A/B family proteins, highlighting areas where the proteins have not been clearly differentiated. Herein, we provide a comprehensive assembly of the literature on these proteins. We find that there are critical gaps in our grasp of A/B proteins’ alternative splice isoforms, structures, regulation, and tissue and cell-type-specific functions, and propose that future mechanistic research integrating multiple A/B proteins will significantly improve our understanding of how this essential protein family contributes to cell homeostasis and disease.

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Selectively RNA interaction by a hnRNPA/B-like protein at presynaptic terminal of squid neuron

Cited by 3 publications

References 29 publications

hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated

hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated

The Biological and Structural Organization of the Squid Brain

hnRNP A/B Proteins: An Encyclopedic Assessment of Their Roles in Homeostasis and Disease

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