Selective Transformation of B Lymphocytes by E.B. Virus

Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein

“…One method which has been used in attempts to produce human monoclonal antibodies is the infection of antibody-producing B lymphocytes with the Epstein-Barr (EB) virus. This virus is a human herpesvirus which only infects B lymphocytes (Pattengale et al, 1973), transforming them into lymphoblastoid cell lines with the capacity for indefinite growth in culture (Pope et al, 1968). These transformed cells synthesize and secrete immunoglobulin (Rosen et al, 1977), and recently this fact has been exploited to produce cell lines which secrete specific antibodies (Steinitz et al, 1977(Steinitz et al, , 1980Kozbor et al, 1979;Kozbor & Roder, 1981).…”

The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma

“…2 Moreover, EBV has the unique ability to transform resting B cells into permanent, latently infected lymphoblastoid cell lines. 3,4 In immunocompetent hosts, EBV infection has been implicated in several lymphoid malignancies, including Burkitt lymphoma, 5 extranodal natural killer (NK)-T-cell lymphomas, 6 aggressive NK leukemia/lymphoma, 7 lymphomatoid granulomatosis, 7 angioimmunoblastic T-cell lymphoma, 8 and a proportion of Hodgkin lymphoma. 9 The prognostic significance of EBV infection in lymphoid malignancies has not been established, however.…”

Identification of an epithelial cell differentiation responsive region within the BZLF1 promoter of the Epstein--Barr virus

“…EBV can infect and replicate within B lymphocytes (Pattengale et al, 1973) and epithelial cells (Sixbey et al, 1984). Replication in, and subsequent shedding of virus from, epithelial cells at the body surface into body fluids provides for an efficient mechanism of spread from one individual to another.…”