Selective sensing of the nucleoside analogue, trifluridine and tipiracil in dosage form and biological matrices

Talaat, Wael; Kaddah, Mohamed M.Y.; Keshk, Reda Mohammed

doi:10.1016/j.talanta.2021.123009

Cited by 6 publications

(10 citation statements)

References 12 publications

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“…A non-linear Stern-Volmer plot usually gives a preliminary indication that the fluorescence quenching process is not caused by a single static or dynamic mechanism but is rather more complex involving both static and dynamic mechanisms as reported in many references [32,40,50]. To provide more depth to the quenching mechanism interpretation, a temperature study was conducted on the lower part of Stern-Volmer curve which is close to linearity and the Stern-Volmer curve was plotted at different temperatures (Figure 3a), Stern-Volmer equation was applied, and the results are presented in Table 1 as follows:…”

Section: Quantum Yieldmentioning

confidence: 96%

“…It is reported that purine and pyrimidine bases possess a mixed static-dynamic quenching process through a photo-induced electron transfer (PET) mechanism, where the electrons of the fluorophore are transferred to the orbital of pyrimidine bases or that the electron orbitals of the fluorophore are occupied by electrons from purine bases, which lead to fluorescence quenching [32,40,[59][60][61][62][63]. The pyrimidine base is represented in the studied drug by the uracil moiety, which is expected to exert both dynamic [62] as well as static Based on the previous evidence, we suggest that the quenching mechanism between reagent 3 and sofosbuvir is a mixed static dynamic mechanism.…”

Section: Uv-visible Absorption Spectra Studymentioning

confidence: 99%

“…Research into fluorescence quenching is promising because of its wide-ranging applications in fields like pharmaceutical, chemical, and biological analysis. Several publications analyzing various pharmaceuticals and chemical substances using the quenching approach have been published [32][33][34][35][36][37][38][39].…”

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confidence: 99%

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A new fluorescence probe for sofosbuvir analysis in dosage form and spiked human plasma

Nasr,

Talaat,

Morshedy

et al. 2024

Luminescence

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A simple, rapid, and low‐cost technique was developed to allow reliable analysis of the anti‐hepatitis C drug sofosbuvir in bulk, tablet form, and spiked human plasma. This method depends on the ability of sofosbuvir to quench the fluorescence of the newly synthesized 2‐amino‐3‐cyano‐4,6‐dimethylpyridine (reagent 3). Elemental analysis and spectral data were used to validate the structure of the synthesized reagent. The newly synthesized reagent exhibited a satisfactory level of fluorescence emission at 365 nm after excitation at 247 nm. All experimental variables that might affect the quenching process were analyzed and optimized. Linearity, range, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) were all validated in accordance with the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines. The concentration range was shown to be linear between 0.1 and 1.5 μg/mL. The technique was effectively utilized for sofosbuvir analysis in both its tablet dosage form and spiked human plasma, with mean percentage recoveries of 100.13 ± 0.35 and 94.26 ± 1.69, respectively.

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Section: Quantum Yieldmentioning

confidence: 96%

Section: Uv-visible Absorption Spectra Studymentioning

confidence: 99%

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A new fluorescence probe for sofosbuvir analysis in dosage form and spiked human plasma

Nasr,

Talaat,

Morshedy

et al. 2024

Luminescence

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“…The present investigation is the first fluororimetic analytical method for the analysis of both drugs, darolutamide and thalidomide. There are several fluorescence-based methods for the detection and quantitation of drugs and chemicals [ 14 , 15 , 16 , 17 ]. The nucleoside analogues, trifluridine and tipiracil, have been recently determined by fluorimetric sensing [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…There are several fluorescence-based methods for the detection and quantitation of drugs and chemicals [ 14 , 15 , 16 , 17 ]. The nucleoside analogues, trifluridine and tipiracil, have been recently determined by fluorimetric sensing [ 14 ]. The tricyclic antidepressant, tianeptine, has been analyzed utilizing its quenching effect over the fluorescence of vilazodone [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Selective Sensing of Darolutamide and Thalidomide in Pharmaceutical Preparations and in Spiked Biofluids

2022

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Selective spectrofluorometric sensing is introduced for the analysis of non-steroidal anti-androgens, darolutamide, and thalidomide in pharmaceutical preparations and biofluids. An organic fluorophore, 2,4,8,10-tetramethylpyrido[2′,3′:3,4]pyrazolo[1,5-a]pyrimidine 2 was synthesized in our laboratories by new simple methods to act as a fluorescent reagent for the analysis of the studied drugs. Elemental and spectral analyses were performed to approve the fluorophore structure. The fluorophore possesses a fluorescence at λem 422 nm when excited at 328 nm. The interaction between the studied drugs and the fluorophore was found to be quenching. The quenching mechanisms were studied and interpreted through the Stern–Volmer relationship. Moreover, the Stern–Volmer constants were calculated for the quenching interactions of both drugs. The introduced method was validated for the estimation of darolutamide and thalidomide in dosage forms, plasma, and urine, offering good percentage recoveries.

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Utility of green chemistry for feasible spectrofluorometric determination of trifluridine via metal complexation: Application to dosage forms

Salem,

Emad,

Fares

et al. 2024

Luminescence

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The goal of the current research was to establish a quick and practical fluorometric technique for trifluridine analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high‐fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 450 nm following excitation at 400 nm. With a determination coefficient of 0.9994, the association between emission intensity and trifluridine concentration was linear between 1 and 125 ng mL−1. The quantitation limit was 0.987 ng mL−1 while 0.333 ng mL−1 was the detection limit. The buffer type, pH and concentration, type of surfactant and concentration, and finally the diluting solvent were among the reaction conditions that were closely examined. With great precision and reliability, the drug in question was quantified using this method in dosage formulations. The proposed method's level of greenness was assessed using two methodologies: the analytical greenness metric (AGREE) and the Green Analytical Procedure Index (GAPI).

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Selective sensing of the nucleoside analogue, trifluridine and tipiracil in dosage form and biological matrices

Cited by 6 publications

References 12 publications

A new fluorescence probe for sofosbuvir analysis in dosage form and spiked human plasma

A new fluorescence probe for sofosbuvir analysis in dosage form and spiked human plasma

Selective Sensing of Darolutamide and Thalidomide in Pharmaceutical Preparations and in Spiked Biofluids

Utility of green chemistry for feasible spectrofluorometric determination of trifluridine via metal complexation: Application to dosage forms

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