Selective release of lysosomal hydrolases from phagocytic cells by cytochalasin B

Davies, P.L.; Allison, A. C.; Haswell, A.David

doi:10.1042/bj1340033

Cited by 37 publications

(10 citation statements)

References 41 publications

(36 reference statements)

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“…The concentration of Na + and K + electrolytes in the extracellular fluid is maintained by the transport mechanisms occurring across the cell membranes and by the kidneys [39]. In agreement with the finding of Allison et al [40], Gava et al [5]; Bhadranna and Ahmed [41,42], the present study recorded…”

Section: Discussionsupporting

confidence: 82%

Egyptian Pyramid Model Housing Enhancing the Compensatory Renal Growth in Nephrectomized Rats

Margaret¹

2016

JCMAH

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The present study aims to evaluate the role of the Egyptian pyramids housing on the enhancement of reno-regenerative ability after unilateral nephrectomy in rats. Forty two male Wistar rats were assigned equally into three main groups (14 rats for each).The sham-operated control (sham group) and unilateral nephrectomized rats (Nx group). Both groups maintained in their home cages for 7 and 14 days. The third group(Pyramid housed group) in which Nx rats housed within the wooden pyramid (6 hr/day)for 7 and 14 days. The present study showed significant increase in size of the remaining kidney, rate of sodium excretion and marked increase in urea, uric acid and creatinine levels in the serum of Nx rats. In addition, significant decrease in renin-angiotensin-aldosterone system was also recorded following nephrectomy for 7 and 14 days. Pyramid environment reversed the actions of nephrectomy in rats and restored the kidney function near the sham levels. In conclusion: The present study recommends using pyramidal shapes for nephrectomized individual's rooms immediately following the surgery and during the recovery periods to avoid kidney impairment as consequence of nephrectomy process.

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Section: Discussionsupporting

confidence: 82%

Egyptian Pyramid Model Housing Enhancing the Compensatory Renal Growth in Nephrectomized Rats

Margaret¹

2016

JCMAH

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“…This contrasts with the work of Allison et al (l971), who showed no inhibition of uptake with colchicine, which nevertheless did prevent saltatory movements of the vacuoles. Cytochalasin B prevented phagocytosis but not micropinocytosis, and it enhanced granule enzyme release as it did in neutrophils (see Davies et al, 1973). Release was inhibited by cAMP and cGMP.…”

Section: Macrophagesmentioning

confidence: 89%

“…Streptolysin 0, Vitamin A, Antineutrophil Antibody and Complement. Streptolysin 0, vitamin A, and antineutrophil antibody and complement exhibit properties similar to those of leukocidin, inducing massive granule enzyme release in a reaction which requires Ca 2 + but is in fact eventually cytotoxic (Woodin and Wieneke, 1970;Davies et at., 1973). Heterologous antineutrophil antibody and complement eventually induce total lysis of the cell.…”

Section: Cytotoxic Reactions Of Neutrophilsmentioning

confidence: 97%

Mechanisms of Mediator Release from Inflammatory Cells

Henson

1974

Mediators of Inflammation

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“…Thereafter the decrease of a-N-acetylglucosaminidase and fl-glucuronidase is much more pronounced than that of P-N-acetylhexosaminidase. Correspondingly, a-N-acetylglucosaminidase and 8-glucuronidase have half-lives of 1.5 [I61 and 3 days [14], respectively, whereas P-N-acetylhexosaminidase persists with a half-life time of 7 days in fibroblasts [22].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Pinocytosis by Cytochalasin B. Decrease in Intracellular Lysosomal-Enzyme Activities and Increased Storage of Glycosaminoglycans

Figura

Kresse

1974

Eur J Biochem

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The influence of Cytochalasin B on the pinocytosis of lysosomal enzymes and on the intracellular accumulation, secretion and uptake of sulfated glycosaminoglycans has been studied in cultivated skin fibroblasts.The uptake of a-N-acetylglucosaminidase was measured in Sanfilippo B fibroblasts, that of P-N-acetylhexosaminidase in Sandhoff fibroblasts and that of P-glucuronidase in fibroblasts from a patient with P-glucuronidase deficiency. Cytochalasin B reduces drastically the uptake of these glycosidases. For a-N-acetylglucosaminidase a dose-response relationship and the time interval between application of the drug and the onset of inhibition of pinocytosis are given.When normal fibroblasts are incubated in the presence of Cytochalasin B the cells become depleted of the intracellular activity of lysosomal hydrolases but not of the cytoplasmic enzyme lactate dehydrogenase. In the medium an increase of P-N-acetylhexosaminidase activity is measurable. The decrease of the activity of intralysosornal enzymes mirrors their intracellular half-life time as determined in mutant cell strains. As a consequence of the lowered hydrolase activity excessive amounts of sulfated glycosaminoglycans are accumulated in normal fibroblasts although the pinocytosis of secreted proteoglycans is markedly diminished.The results support the hypothesis that in fibroblasts lysosomal enzymes are primarily secreted and reach thereafter the lysosomes by adsorptive pinocytosis.The mold metabolite Cytochalasin B has gained widespread interest in cell biology (for reviews see [1,2]) since Carter [3] first reported its inhibitory effect on the motility and cytokinesis of cultured cells. Cytochalasin B interferes with the function of cytoplasmic contractile microfilaments, inhibits the transport of sugars and nucleosides in fibroblasts and the phagocytosis by macrophages [3-81.With regard to the effect of Cytochalasin B on pinocytosis contradictory results have been reported. An inhibition of pinocytosis of sucrose by Chang cells [9], of the uptake of antiglobulin-receptor complexes by lymphocytes [lo], and of the formation of pinosomes in macrophages [5] has been described, whereas no effect on the endocytosis of ferritin [ l l ] and of bovine serum albumin [I21 by macrophages could be observed.Recent studies on the possibility of enzyme replacement therapy in lysosomal storage disorders Enzymes. /I-N-Acetylhexosaminidase (EC 3.2.1.52); a-Nacetylglucosaminidase (EC 3.2.1.50) ; /I-glucuronidase (EC 3.2.1.31); a-L-iduronidase (EC 3.2.1.76).[13] and on the pathogenesis of I-cell disease [14] stress the need for studying the pinocytotic uptake of lysosomal hydrolases. We have therefore used Cytochalasin B as a tool for investigating the endocytosis of several lysosomal glycosidases in cultured human fibroblasts. It will be shown in this paper that the drug inhibits the uptake of these enzymes and also of sulfated glycosaminoglycans by the cells. The inhibition of pinocytosis is followed by a decrease of the normal intracellular activity of those hydr...

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Selective release of lysosomal hydrolases from phagocytic cells by cytochalasin B

Cited by 37 publications

References 41 publications

Egyptian Pyramid Model Housing Enhancing the Compensatory Renal Growth in Nephrectomized Rats

Egyptian Pyramid Model Housing Enhancing the Compensatory Renal Growth in Nephrectomized Rats

Mechanisms of Mediator Release from Inflammatory Cells

Inhibition of Pinocytosis by Cytochalasin B. Decrease in Intracellular Lysosomal-Enzyme Activities and Increased Storage of Glycosaminoglycans

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